FastQCFastQC Report
Thu 2 Feb 2017
SRR4062825_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062825_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670335
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT122670.4593805646108073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT77340.28962658243254125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA54730.20495555801051177No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC43370.1624140791323935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT42960.16087869125034873No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34510.12923472148625548No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA29690.11118455175099753No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29620.11092241235650209No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28250.10579196992137689No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27400.10260884870250363No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC27390.1025714002175757No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27180.10178498203408937No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27130.10159773960944975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG6950.017.95558528
CTAGCGG7300.017.0953429
TAGCGGC7250.016.99255630
CGGTCCA7450.016.75145310
CAAGACG7800.016.407274
TAACGTC703.7043434E-415.9967895
TAGGACG6200.015.7390714
AAGACGG8150.015.506095
GTCCTAT8950.015.3908131
CCTATAC1901.8189894E-1215.1559893
CCGGTCC8450.015.1477259
GATATAC6050.015.090571
AGCGGCG8350.014.94562731
CCTATTC8250.014.7375393
GTCCTAC7200.014.6824181
CGCCGGT7800.014.5630887
GTAGGAC7450.014.3874523
CTATTCC8500.014.3032794
CAACGTA2350.014.29741330
AACGCTT7050.014.29741130
Produced by FastQC (version 0.11.5)