##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062825_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2670335
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.150249687773258	32.0	32.0	32.0	32.0	32.0
2	30.736445427259127	32.0	32.0	32.0	32.0	32.0
3	30.800865434486685	32.0	32.0	32.0	32.0	32.0
4	30.865144635410914	32.0	32.0	32.0	32.0	32.0
5	30.771551883939654	32.0	32.0	32.0	32.0	32.0
6	34.41618860554949	36.0	36.0	36.0	32.0	36.0
7	34.32045230280096	36.0	36.0	36.0	32.0	36.0
8	34.30579571476987	36.0	36.0	36.0	32.0	36.0
9	34.44287626833337	36.0	36.0	36.0	32.0	36.0
10	34.148982431043294	36.0	36.0	36.0	32.0	36.0
11	34.46075005570462	36.0	36.0	36.0	32.0	36.0
12	34.26084592382604	36.0	36.0	36.0	32.0	36.0
13	34.35022684419745	36.0	36.0	36.0	32.0	36.0
14	34.24800708525335	36.0	36.0	36.0	32.0	36.0
15	34.19533654017192	36.0	36.0	36.0	32.0	36.0
16	34.202524402368994	36.0	36.0	36.0	32.0	36.0
17	34.131277910823925	36.0	36.0	36.0	32.0	36.0
18	34.15956836876272	36.0	36.0	36.0	32.0	36.0
19	34.13911887459813	36.0	36.0	36.0	32.0	36.0
20	34.11127779847847	36.0	36.0	36.0	32.0	36.0
21	34.08923824164384	36.0	36.0	36.0	32.0	36.0
22	34.066739191899146	36.0	36.0	36.0	32.0	36.0
23	34.007016722620946	36.0	36.0	36.0	32.0	36.0
24	33.99943040854425	36.0	36.0	36.0	32.0	36.0
25	33.96294247725473	36.0	36.0	36.0	32.0	36.0
26	33.923214128564396	36.0	36.0	36.0	32.0	36.0
27	33.917055725218	36.0	36.0	36.0	32.0	36.0
28	33.89635195584075	36.0	36.0	36.0	32.0	36.0
29	33.863588650862155	36.0	36.0	36.0	32.0	36.0
30	33.82630756066187	36.0	36.0	36.0	32.0	36.0
31	33.849582168529416	36.0	36.0	36.0	32.0	36.0
32	33.79719473399405	36.0	36.0	36.0	32.0	36.0
33	33.74627827594665	36.0	36.0	36.0	32.0	36.0
34	33.74269745181784	36.0	36.0	36.0	32.0	36.0
35	33.69444807486701	36.0	36.0	36.0	27.0	36.0
36	33.64935822658955	36.0	36.0	36.0	27.0	36.0
37	33.63226823600784	36.0	36.0	36.0	27.0	36.0
38	33.01784982034089	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	6.0
9	9.0
10	29.0
11	28.0
12	20.0
13	16.0
14	478.0
15	904.0
16	1167.0
17	1405.0
18	1708.0
19	2253.0
20	2876.0
21	4251.0
22	6231.0
23	9124.0
24	13580.0
25	20175.0
26	29442.0
27	41132.0
28	57629.0
29	77567.0
30	102017.0
31	137197.0
32	184057.0
33	270272.0
34	578968.0
35	1127787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.169078412323586	17.67750261809355	12.21462030425753	24.938798665325344
2	15.916684433430092	20.121018824276156	38.61031811186953	25.351978630424217
3	18.648430714630546	24.920216461419823	28.62900401754732	27.80234880640231
4	12.294134984010903	15.994790014594102	36.45521760639519	35.25585739499981
5	14.224967288638165	37.03779987911589	33.502201603849116	15.235031228396833
6	33.09151534942524	36.083619690165406	17.43426275723432	13.390602203175039
7	29.700677780636347	31.113947211827135	21.047374990310196	18.138000017226314
8	27.982784111590952	32.853766059848915	19.812240012822414	19.351209815737715
9	27.692216087312122	14.651907930411545	18.871089993439007	38.78478598883733
10	15.866257277683097	26.84373901115259	31.619419537950684	25.670584173213634
11	36.65941147568487	21.745013242096686	22.585526867674904	19.010048414543537
12	25.044948957981855	24.09335302186948	28.9361187393994	21.925579280749265
13	29.230943823625026	20.128594264757215	25.66188554983093	24.97857636178682
14	23.719578124526	20.397539097187085	25.637837410681414	30.245045367605506
15	24.830009562839994	27.378489565716713	23.572681516974324	24.21881935446897
16	25.180924730143783	26.32263346557889	24.544977533985534	23.95146427029179
17	23.33847981709382	26.23148539357491	26.08214746960803	24.347887319723245
18	24.410759594408606	25.09659479247615	27.504639209040604	22.98800640407464
19	24.903758826867005	25.554330056945112	26.3515241852879	23.19038693089998
20	25.09437636695928	24.50062917577973	26.519571860862268	23.885422596398715
21	26.607705962983214	24.52215988674751	25.143026208363604	23.72710794190567
22	25.040597243881574	24.85862122172237	25.960177249664063	24.14060428473199
23	23.637655398780407	24.843131218479243	26.316203419690748	25.203009963049606
24	24.051833335705417	25.86191010890873	26.08554792576018	24.00070862962566
25	24.593933364845338	24.91626970779982	26.0200263532246	24.46977057413024
26	23.70248182761035	25.951187465049497	26.828016736539585	23.518313970800577
27	24.634084134526255	25.44413446633267	25.948051763223663	23.97372963591741
28	23.628163245587064	25.362478142703754	26.646146056361395	24.363212555347786
29	23.508909781602767	25.555131509118016	26.726460959617505	24.20949774966171
30	23.80978060798212	25.623772817679892	26.845758981485957	23.720687592852034
31	24.15849022403434	25.58914766132955	25.636298199628417	24.61606391500769
32	23.65073123232777	25.868022395745555	25.68177817725596	24.799468194670713
33	23.453243768782038	25.36886203683452	26.227255567573398	24.950638626810044
34	24.205624663154296	25.78454384303168	26.420238869719036	23.589592624094987
35	24.904209536722487	25.486930173153198	26.365093692998588	23.243766597125724
36	23.69989198226771	26.233776740381405	25.859085549744336	24.207245727606548
37	24.728826115387754	26.02355454450802	25.68210128768738	23.565518052416838
38	23.78656993331481	26.05093031947922	25.89378606060016	24.26871368660581
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	477.0
1	565.0
2	653.0
3	653.0
4	1600.5
5	2548.0
6	2548.0
7	3143.0
8	3738.0
9	3648.0
10	3558.0
11	3558.0
12	4597.5
13	5637.0
14	7312.5
15	8988.0
16	8988.0
17	13308.5
18	17629.0
19	17629.0
20	21288.0
21	24947.0
22	25796.5
23	26646.0
24	26646.0
25	31551.5
26	36457.0
27	36457.0
28	46153.5
29	55850.0
30	66782.0
31	77714.0
32	77714.0
33	92872.5
34	108031.0
35	108031.0
36	117782.5
37	127534.0
38	143073.5
39	158613.0
40	158613.0
41	166061.5
42	173510.0
43	192248.0
44	210986.0
45	210986.0
46	220678.0
47	230370.0
48	230370.0
49	235708.5
50	241047.0
51	235859.0
52	230671.0
53	230671.0
54	218223.0
55	205775.0
56	205775.0
57	198365.5
58	190956.0
59	171525.5
60	152095.0
61	152095.0
62	143350.5
63	134606.0
64	111798.5
65	88991.0
66	88991.0
67	74263.0
68	59535.0
69	59535.0
70	47903.5
71	36272.0
72	28161.0
73	20050.0
74	20050.0
75	15043.5
76	10037.0
77	10037.0
78	9772.5
79	9508.0
80	7440.5
81	5373.0
82	5373.0
83	5320.5
84	5268.0
85	5268.0
86	3547.5
87	1827.0
88	1605.5
89	1384.0
90	1384.0
91	1043.5
92	703.0
93	605.5
94	508.0
95	508.0
96	500.5
97	493.0
98	493.0
99	921.5
100	1350.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12425407299084198
2	0.055948036482314015
3	0.011159648508520466
4	0.0029209818243778403
5	3.370363643512893E-4
6	2.621393944954472E-4
7	7.489696985584206E-5
8	4.1193333420713133E-4
9	2.621393944954472E-4
10	5.242787889908944E-4
11	0.0023592545504590246
12	0.0031456727339453667
13	0.014492563667105437
14	0.009661709111403625
15	0.023442751564878562
16	0.01130944244823215
17	0.020634115195284485
18	0.005991757588467365
19	0.008238666684142627
20	0.00655348486238618
21	0.0073024545609446006
22	0.0076020424403679685
23	0.010073642445610757
24	0.014717254576672965
25	0.016964163672348227
26	0.01827486064482546
27	0.009511915171691941
28	0.006516036377458259
29	0.01093495759895294
30	0.0034452606133687346
31	0.0061415515281790485
32	0.00692796971166539
33	0.008650600018349757
34	0.012882278815204833
35	0.016664575792924855
36	0.015091739425952174
37	0.010373230325034125
38	0.006628381832242022
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2670335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.767520491037686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95983874059546	33.391909145830084
2	14.477592049621531	13.252069815838757
3	5.526074427542093	7.587441737925964
4	2.5131773424447577	4.600875820718084
5	1.3144979834243142	3.008065669590001
6	0.791436423352235	2.1733249633876195
7	0.49771316868713783	1.5945368352583515
8	0.3452980338395846	1.264274787141457
9	0.25133834705020036	1.0352819653923226
>10	1.1270734771921593	9.040265856428684
>50	0.08503136299496898	2.7146527211154634
>100	0.08808570039696084	8.725108943223399
>500	0.014655508142397028	4.593974922517327
>1k	0.00794181167492853	6.063658689266626
>5k	1.6374869432842328E-4	0.4948908367320545
>10k+	8.187434716421164E-5	0.4596672896336876
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12267	0.4593805646108073	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7734	0.28962658243254125	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	5473	0.20495555801051177	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4337	0.1624140791323935	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4296	0.16087869125034873	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3451	0.12923472148625548	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2969	0.11118455175099753	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2962	0.11092241235650209	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2825	0.10579196992137689	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2740	0.10260884870250363	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2739	0.1025714002175757	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2718	0.10178498203408937	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2713	0.10159773960944975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1234545478376308E-4
2	0.0	0.0	0.0	0.0	1.1234545478376308E-4
3	0.0	0.0	0.0	0.0	1.1234545478376308E-4
4	0.0	0.0	0.0	7.489696985584206E-5	1.1234545478376308E-4
5	0.0	0.0	0.0	7.489696985584206E-5	1.1234545478376308E-4
6	0.0	0.0	0.0	7.489696985584206E-5	1.1234545478376308E-4
7	0.0	0.0	0.0	1.4979393971168413E-4	1.1234545478376308E-4
8	0.0	0.0	0.0	1.4979393971168413E-4	1.1234545478376308E-4
9	0.0	0.0	0.0	3.3703636435128924E-4	1.1234545478376308E-4
10	0.0	0.0	0.0	3.3703636435128924E-4	1.1234545478376308E-4
11	0.0	0.0	0.0	4.493818191350523E-4	1.1234545478376308E-4
12	0.0	0.0	0.0	4.8683030406297337E-4	3.3703636435128924E-4
13	0.0	0.0	0.0	4.8683030406297337E-4	4.493818191350523E-4
14	0.0	0.0	0.0	4.8683030406297337E-4	4.493818191350523E-4
15	0.0	0.0	0.0	4.8683030406297337E-4	6.740727287025785E-4
16	0.0	0.0	0.0	5.617272739188154E-4	6.740727287025785E-4
17	3.744848492792103E-5	0.0	0.0	6.366242437746575E-4	6.740727287025785E-4
18	3.744848492792103E-5	0.0	0.0	7.489696985584206E-4	7.864181834863416E-4
19	3.744848492792103E-5	0.0	0.0	8.238666684142627E-4	8.238666684142627E-4
20	3.744848492792103E-5	0.0	0.0	8.987636382701046E-4	9.362121231980257E-4
21	3.744848492792103E-5	0.0	0.0	9.736606081259467E-4	0.0010485575779817888
22	3.744848492792103E-5	0.0	0.0	0.001348145457405157	0.0010860060629097098
23	3.744848492792103E-5	0.0	0.0	0.002359254550459025	0.0010860060629097098
24	3.744848492792103E-5	0.0	0.0	0.004081884857143392	0.0010860060629097098
25	3.744848492792103E-5	0.0	0.0	0.004531266676278445	0.0010860060629097098
26	3.744848492792103E-5	0.0	0.0	0.005916860618611523	0.0011609030327655518
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	695	0.0	17.955585	28
CTAGCGG	730	0.0	17.09534	29
TAGCGGC	725	0.0	16.992556	30
CGGTCCA	745	0.0	16.751453	10
CAAGACG	780	0.0	16.40727	4
TAACGTC	70	3.7043434E-4	15.996789	5
TAGGACG	620	0.0	15.739071	4
AAGACGG	815	0.0	15.50609	5
GTCCTAT	895	0.0	15.390813	1
CCTATAC	190	1.8189894E-12	15.155989	3
CCGGTCC	845	0.0	15.147725	9
GATATAC	605	0.0	15.09057	1
AGCGGCG	835	0.0	14.945627	31
CCTATTC	825	0.0	14.737539	3
GTCCTAC	720	0.0	14.682418	1
CGCCGGT	780	0.0	14.563088	7
GTAGGAC	745	0.0	14.387452	3
CTATTCC	850	0.0	14.303279	4
CAACGTA	235	0.0	14.297413	30
AACGCTT	705	0.0	14.297411	30
>>END_MODULE