FastQCFastQC Report
Thu 2 Feb 2017
SRR4062825_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062825_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670335
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT137120.5134936253316531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT92400.3460240007339903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT54040.20237161255048522No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA53690.20106091557800798No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC52840.19787779435913472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.15376347911404376No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC30720.1150417456985734No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30700.11496684872871757No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29840.11174627902491635No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29730.11133434569070921No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA28480.10665328507471908No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27550.10317057597642243No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27510.10302078203671075No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA27060.10133560021495432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5350.018.84022928
TATACTG2600.017.8483165
TAGCGGC5700.017.6833730
CTAGCGG5850.017.22995429
TAGGACG6100.016.2633514
ACCGTCG5550.015.8583718
CGTTTAT6000.015.73258731
ATCGTTT6050.015.60256629
GTATTAG5850.015.5901731
CGTCGTA5700.015.43988810
TACCGTC5550.015.2817027
CCGTCGT5850.015.0442769
GTCCTAC6500.015.0157971
AGCGGCG7050.014.97801331
TTAGACG756.2451087E-414.9343034
AGGCCCG8750.014.81266910
ATACCGT5850.014.7715746
TAGACAG4550.014.771025
GTAGGAC8050.014.7084793
CGAGCCG8750.014.62787715
Produced by FastQC (version 0.11.5)