FastQCFastQC Report
Thu 2 Feb 2017
SRR4062824_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062824_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447426
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49980.34530262687004376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34830.24063406350307376No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21520.14867772169354426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18310.1265004221286615No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16530.1142027295350505No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16050.11088649782441383No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15860.1095738227722868No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15550.10743208979250062No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15410.10646485554356491No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.10598123841909707No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15260.10542853313399096No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15140.10459947520633178No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14990.10356315279675783No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14870.10273409486909865No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14610.1009378026925038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC3150.017.7764727
ATACCGT3300.017.4502496
CCGTCGT3150.016.763579
ACCGTCG3250.016.244968
CGTCGTA3400.016.00158910
TTAGTAC600.0024446415.9977213
TGTACCG805.6343233E-515.9960625
CAAGACG4350.015.81224
TCTAGCG3650.015.77857328
GCGCAAG4200.015.6346481
CTAGCGG3800.015.57726529
TCGTTTA3200.015.49886230
TAGCGGC3750.015.35887230
AAGACGG4400.015.2689695
CGCAAGA4300.015.2631332
ATCGTTT3250.015.25989129
AACCGCG2100.015.2369777
TCTTATA2000.015.2072922
CGCGGTC2000.015.2015110
ACGGACC4550.015.119778
Produced by FastQC (version 0.11.5)