FastQCFastQC Report
Thu 2 Feb 2017
SRR4062824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447426
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59300.40969279258490593No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44090.3046097002541063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT25590.17679660307331774No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20520.14176890562971783No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20140.1391435555254638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.13734726334886896No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18380.12698403925312934No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17200.1188316362978142No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16230.11213008471590256No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16090.11116285046696689No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15420.10653394370420319No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15190.10494491600952312No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14750.1019050369414395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT1001.4551915E-1020.801466
GTATAAT851.05628715E-820.7073361
TACCGTC3350.017.673497
ATACCGT3550.017.57876
TAGACGT652.1003057E-417.2313844
CGCAAGA4000.017.1994252
ATCGTTT3350.017.19345529
AAGACGG4100.017.1719365
TAGGACG5850.016.9578694
ACGAACG1151.8426363E-816.69509315
CATCGTT3550.016.67549928
TCTAGCG3750.016.63944228
CAAGACG4850.016.4954344
ACCGTCG3400.016.4723138
TTAGGAC3550.016.225373
TCGTTTA3550.016.2248130
CTAGCGG3950.016.20198829
CCGTCGT3400.016.0016759
CGAGCGC703.6952493E-416.00112310
CGGTCCA4100.016.00112310
Produced by FastQC (version 0.11.5)