##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1447426
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.151354887918277	32.0	32.0	32.0	32.0	32.0
2	31.286657141712254	32.0	32.0	32.0	32.0	32.0
3	31.374275437915305	32.0	32.0	32.0	32.0	32.0
4	31.48148713647537	32.0	32.0	32.0	32.0	32.0
5	31.397511858982774	32.0	32.0	32.0	32.0	32.0
6	34.92108059410291	36.0	36.0	36.0	36.0	36.0
7	34.949645785000406	36.0	36.0	36.0	36.0	36.0
8	34.88900848817141	36.0	36.0	36.0	36.0	36.0
9	34.990872072216476	36.0	36.0	36.0	36.0	36.0
10	34.848801251324765	36.0	36.0	36.0	32.0	36.0
11	35.02203083266433	36.0	36.0	36.0	36.0	36.0
12	34.92253213635792	36.0	36.0	36.0	32.0	36.0
13	34.97344596545868	36.0	36.0	36.0	36.0	36.0
14	34.910350511874185	36.0	36.0	36.0	32.0	36.0
15	34.88698627770953	36.0	36.0	36.0	32.0	36.0
16	34.89504955693763	36.0	36.0	36.0	32.0	36.0
17	34.86826062265014	36.0	36.0	36.0	32.0	36.0
18	34.85779238455023	36.0	36.0	36.0	32.0	36.0
19	34.85089945876335	36.0	36.0	36.0	32.0	36.0
20	34.838189309850726	36.0	36.0	36.0	32.0	36.0
21	34.82868070630209	36.0	36.0	36.0	32.0	36.0
22	34.81129328891425	36.0	36.0	36.0	32.0	36.0
23	34.74898820388745	36.0	36.0	36.0	32.0	36.0
24	34.730263239709664	36.0	36.0	36.0	32.0	36.0
25	34.710207637557986	36.0	36.0	36.0	32.0	36.0
26	34.645738711339995	36.0	36.0	36.0	32.0	36.0
27	34.62917620659018	36.0	36.0	36.0	32.0	36.0
28	34.59558208847982	36.0	36.0	36.0	32.0	36.0
29	34.55947592484866	36.0	36.0	36.0	32.0	36.0
30	34.53306421191826	36.0	36.0	36.0	32.0	36.0
31	34.52249510510382	36.0	36.0	36.0	32.0	36.0
32	34.490097593935715	36.0	36.0	36.0	32.0	36.0
33	34.45510858586208	36.0	36.0	36.0	32.0	36.0
34	34.43397244487801	36.0	36.0	36.0	32.0	36.0
35	34.39164903767101	36.0	36.0	36.0	32.0	36.0
36	34.354056787704515	36.0	36.0	36.0	32.0	36.0
37	34.33868121755447	36.0	36.0	36.0	32.0	36.0
38	33.91801169800736	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	20.0
22	124.0
23	377.0
24	956.0
25	2455.0
26	5249.0
27	9767.0
28	17333.0
29	27351.0
30	41163.0
31	59141.0
32	83812.0
33	133367.0
34	322541.0
35	743761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1537956084851	18.339850106163862	12.033101843374967	26.47325244197608
2	15.355890633366151	20.835898233069074	37.44228543793288	26.365925695631898
3	18.062339629107118	24.855156671221877	28.84465250727844	28.237851192392565
4	11.811031782111318	16.261922955220953	36.39314973283461	35.533895529833124
5	13.676770817140785	37.41948632917077	33.57127312295974	15.332469730728707
6	33.83917486456966	36.26721421450399	16.748939321815392	13.144671599110971
7	29.58128313650062	30.886154832522926	21.093640969945184	18.43892106103127
8	27.82854714849782	33.56236549110644	19.501794603408193	19.107292756987555
9	27.437885656131012	13.8727389176652	18.573606905209513	40.11576852099427
10	15.403620113955036	27.232655779656533	32.04227127986276	25.32145282652567
11	37.49308217034339	21.039944118445682	22.433113828266478	19.033859882944448
12	24.58300027013527	23.985110088757743	29.218825457381843	22.213064183725145
13	29.84308675274142	19.595847519455255	25.332798129642732	25.228267598160592
14	23.22101440764502	20.076259511712514	25.70045031663104	31.002275764011422
15	24.828834082018698	27.914864041408684	22.73746637133781	24.518835505234808
16	25.10028146516644	25.930997508680925	24.690657760742173	24.27806326541046
17	23.512082828414027	26.214880760743554	25.89666069284371	24.376375717998712
18	24.497003646473118	24.748622727517677	27.412454937247222	23.341918688761982
19	25.20260103107171	25.137105454786635	26.120091804347854	23.5402017097938
20	25.37124523118971	24.30542217702321	26.158297557180816	24.165035034606262
21	26.33851587371772	24.403491996816413	25.048430867527415	24.20956126193845
22	25.19189236617278	24.98925679102075	25.707082780052314	24.11176806275416
23	23.65652611572429	24.51246111192098	26.382681496701377	25.448331275653352
24	24.240962923147713	25.39349161891523	26.159472055911664	24.20607340202539
25	24.47303005473164	24.76278580044852	26.114841104139348	24.649343040680492
26	23.85697092632024	25.693057883442744	26.559837946810404	23.890133243426607
27	24.77551513895366	25.177746688084014	25.778468659861474	24.26826951310085
28	23.78131939042134	25.07278437723241	26.560183387613602	24.585712844732647
29	23.854414664376623	25.17144227062385	26.386288487287086	24.587854577712438
30	23.837004447895783	25.30726959443868	26.77131680652413	24.084409151141408
31	24.541220069281607	25.152235761966413	25.501752766635395	24.804791402116585
32	23.9366986636968	25.512461431534323	25.68027657372467	24.870563331044213
33	23.684457789206494	24.91692148683249	26.3776524672073	25.02096825675371
34	24.42404654883911	25.346718934163125	26.47741577116896	23.7518187458288
35	25.163497132150454	25.11223371695686	26.252188367488216	23.47208078340447
36	23.87686831658406	25.845120925007564	25.95075672262347	24.327254035784904
37	24.811907482662328	25.62721686635448	25.520544746328998	24.04033090465419
38	23.978859008238768	25.53893984144256	26.015233949945593	24.466967200373077
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	118.0
2	175.0
3	175.0
4	659.0
5	1143.0
6	1143.0
7	1474.0
8	1805.0
9	1875.5
10	1946.0
11	1946.0
12	2531.5
13	3117.0
14	3934.5
15	4752.0
16	4752.0
17	6868.5
18	8985.0
19	8985.0
20	10431.5
21	11878.0
22	12263.5
23	12649.0
24	12649.0
25	14666.5
26	16684.0
27	16684.0
28	21682.5
29	26681.0
30	32946.0
31	39211.0
32	39211.0
33	48738.0
34	58265.0
35	58265.0
36	63141.0
37	68017.0
38	77057.5
39	86098.0
40	86098.0
41	89684.5
42	93271.0
43	103239.0
44	113207.0
45	113207.0
46	119009.0
47	124811.0
48	124811.0
49	128789.5
50	132768.0
51	130967.0
52	129166.0
53	129166.0
54	122575.0
55	115984.0
56	115984.0
57	112067.0
58	108150.0
59	96362.5
60	84575.0
61	84575.0
62	79645.5
63	74716.0
64	61594.0
65	48472.0
66	48472.0
67	40359.5
68	32247.0
69	32247.0
70	25893.0
71	19539.0
72	15431.5
73	11324.0
74	11324.0
75	8467.5
76	5611.0
77	5611.0
78	5467.0
79	5323.0
80	4114.5
81	2906.0
82	2906.0
83	2740.5
84	2575.0
85	2575.0
86	1651.5
87	728.0
88	582.5
89	437.0
90	437.0
91	259.5
92	82.0
93	59.5
94	37.0
95	37.0
96	26.0
97	15.0
98	15.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009326901686165649
2	6.908816063826407E-5
3	0.0
4	5.527052851061126E-4
5	6.908816063826407E-5
6	2.072644819147922E-4
7	2.763526425530563E-4
8	0.002141732979786186
9	0.0037998488351045235
10	8.981460882974328E-4
11	0.005457964690422861
12	2.072644819147922E-4
13	1.3817632127652814E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3817632127652814E-4
22	0.0
23	2.072644819147922E-4
24	0.0
25	0.0
26	0.0
27	6.908816063826407E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.908816063826407E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1447426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.01658836147028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79976467209214	41.4846127455019
2	14.381689325359595	15.536995844610013
3	4.367015975501916	7.076739129499547
4	1.7033057248374777	3.680270567691272
5	0.8440975518642733	2.2797634997988636
6	0.46998455468402567	1.5232177335969563
7	0.2831394547678995	1.0705959163962133
8	0.19465799826863264	0.8411808770995621
9	0.1324297417326183	0.6438062561387999
>10	0.6611105259007992	6.519508346507924
>50	0.06911331083974363	2.687368344846947
>100	0.0783763244186951	8.940289571509826
>500	0.011196563501773511	4.037771010833251
>1k	0.003989580098333091	3.2656428043472756
>5k	1.2869613220429325E-4	0.41223735162166525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5930	0.40969279258490593	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4409	0.3046097002541063	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2559	0.17679660307331774	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2052	0.14176890562971783	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2014	0.1391435555254638	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1988	0.13734726334886896	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1838	0.12698403925312934	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1720	0.1188316362978142	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1623	0.11213008471590256	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1609	0.11116285046696689	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1542	0.10653394370420319	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1519	0.10494491600952312	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1475	0.1019050369414395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.908816063826406E-5
2	0.0	0.0	0.0	0.0	6.908816063826406E-5
3	0.0	0.0	0.0	0.0	6.908816063826406E-5
4	0.0	0.0	0.0	0.0	6.908816063826406E-5
5	0.0	0.0	0.0	0.0	6.908816063826406E-5
6	0.0	0.0	0.0	0.0	1.381763212765281E-4
7	0.0	0.0	0.0	0.0	1.381763212765281E-4
8	0.0	0.0	0.0	0.0	1.381763212765281E-4
9	0.0	0.0	0.0	0.0	1.381763212765281E-4
10	0.0	0.0	0.0	0.0	1.381763212765281E-4
11	0.0	0.0	0.0	0.0	1.381763212765281E-4
12	0.0	0.0	0.0	0.0	2.763526425530562E-4
13	0.0	0.0	0.0	0.0	2.763526425530562E-4
14	0.0	0.0	0.0	1.381763212765281E-4	2.763526425530562E-4
15	0.0	0.0	0.0	1.381763212765281E-4	4.145289638295844E-4
16	0.0	0.0	0.0	2.072644819147922E-4	4.145289638295844E-4
17	0.0	0.0	0.0	2.072644819147922E-4	4.145289638295844E-4
18	0.0	0.0	0.0	2.072644819147922E-4	4.145289638295844E-4
19	0.0	0.0	0.0	2.763526425530562E-4	4.145289638295844E-4
20	0.0	0.0	0.0	3.4544080319132034E-4	4.8361712446784845E-4
21	0.0	0.0	0.0	5.527052851061125E-4	4.8361712446784845E-4
22	0.0	0.0	0.0	5.527052851061125E-4	5.527052851061125E-4
23	0.0	0.0	0.0	0.0014508513734035452	6.217934457443766E-4
24	0.0	0.0	0.0	0.0026253501042540345	6.908816063826407E-4
25	0.0	0.0	0.0	0.003247143549998411	7.599697670209047E-4
26	0.0	0.0	0.0	0.004007113317019316	8.290579276591688E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGCGT	100	1.4551915E-10	20.80146	6
GTATAAT	85	1.05628715E-8	20.707336	1
TACCGTC	335	0.0	17.67349	7
ATACCGT	355	0.0	17.5787	6
TAGACGT	65	2.1003057E-4	17.231384	4
CGCAAGA	400	0.0	17.199425	2
ATCGTTT	335	0.0	17.193455	29
AAGACGG	410	0.0	17.171936	5
TAGGACG	585	0.0	16.957869	4
ACGAACG	115	1.8426363E-8	16.695093	15
CATCGTT	355	0.0	16.675499	28
TCTAGCG	375	0.0	16.639442	28
CAAGACG	485	0.0	16.495434	4
ACCGTCG	340	0.0	16.472313	8
TTAGGAC	355	0.0	16.22537	3
TCGTTTA	355	0.0	16.22481	30
CTAGCGG	395	0.0	16.201988	29
CCGTCGT	340	0.0	16.001675	9
CGAGCGC	70	3.6952493E-4	16.001123	10
CGGTCCA	410	0.0	16.001123	10
>>END_MODULE