FastQCFastQC Report
Thu 2 Feb 2017
SRR4062823_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062823_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306837
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43620.3337830196114741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30590.23407662929653814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17930.13720150255923272No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13940.10666976830316253No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13550.10368546345106544No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13490.10322633962766589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG3600.016.4446815
GTCTTAC908.503805E-616.0155541
GTATAGG1101.997596E-716.0155531
GTCCTAT4300.015.6430971
TAGCGTA2150.015.6269357
ACCGCAG3850.015.37625629
ATAGCGT2200.015.2694416
AATAGCG2300.014.6055525
GTATAGA1103.5075773E-614.5595931
TGTAGGA2750.014.5523472
ACGAACG1553.1322998E-914.45182115
CTAGCGG3100.014.45126829
ACCGTCG1801.1823431E-1014.2213378
AGGCCCG4400.014.18202110
CGACCCG2053.6379788E-1214.0457565
CCGATAA1604.8967195E-914.00029
TCCGATA1604.9021764E-913.9991298
GTCTTAA1952.7284841E-1113.9622761
GTAGGAC2750.013.9611663
GTATTAG2550.013.817341
Produced by FastQC (version 0.11.5)