##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062823_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306837
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2604793099675	32.0	32.0	32.0	32.0	32.0
2	30.93398641146524	32.0	32.0	32.0	32.0	32.0
3	30.99260274999866	32.0	32.0	32.0	32.0	32.0
4	31.046374566988845	32.0	32.0	32.0	32.0	32.0
5	30.985802360967742	32.0	32.0	32.0	32.0	32.0
6	34.636709092258634	36.0	36.0	36.0	32.0	36.0
7	34.565439301152324	36.0	36.0	36.0	32.0	36.0
8	34.550729739056976	36.0	36.0	36.0	32.0	36.0
9	34.66210323093087	36.0	36.0	36.0	32.0	36.0
10	34.428015888745115	36.0	36.0	36.0	32.0	36.0
11	34.67125739476308	36.0	36.0	36.0	32.0	36.0
12	34.51075688857907	36.0	36.0	36.0	32.0	36.0
13	34.57812718801197	36.0	36.0	36.0	32.0	36.0
14	34.49603584838813	36.0	36.0	36.0	32.0	36.0
15	34.45054432955296	36.0	36.0	36.0	32.0	36.0
16	34.460078800952225	36.0	36.0	36.0	32.0	36.0
17	34.40328135796584	36.0	36.0	36.0	32.0	36.0
18	34.42020772292183	36.0	36.0	36.0	32.0	36.0
19	34.408982145439715	36.0	36.0	36.0	32.0	36.0
20	34.37355844684532	36.0	36.0	36.0	32.0	36.0
21	34.35636119883352	36.0	36.0	36.0	32.0	36.0
22	34.33546800404335	36.0	36.0	36.0	32.0	36.0
23	34.294653426555875	36.0	36.0	36.0	32.0	36.0
24	34.28446623412101	36.0	36.0	36.0	32.0	36.0
25	34.261509277744665	36.0	36.0	36.0	32.0	36.0
26	34.22432177846204	36.0	36.0	36.0	32.0	36.0
27	34.226462060685456	36.0	36.0	36.0	32.0	36.0
28	34.20862433494001	36.0	36.0	36.0	32.0	36.0
29	34.18085040444983	36.0	36.0	36.0	32.0	36.0
30	34.1503508088614	36.0	36.0	36.0	32.0	36.0
31	34.16109201070983	36.0	36.0	36.0	32.0	36.0
32	34.12367265389639	36.0	36.0	36.0	32.0	36.0
33	34.07974598209264	36.0	36.0	36.0	32.0	36.0
34	34.07605615696525	36.0	36.0	36.0	32.0	36.0
35	34.02816495094645	36.0	36.0	36.0	32.0	36.0
36	33.99250097755114	36.0	36.0	36.0	32.0	36.0
37	33.9883313680283	36.0	36.0	36.0	32.0	36.0
38	33.39601495825416	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	12.0
11	23.0
12	11.0
13	6.0
14	240.0
15	454.0
16	615.0
17	620.0
18	745.0
19	920.0
20	1197.0
21	1567.0
22	2359.0
23	3523.0
24	5236.0
25	7522.0
26	10867.0
27	16144.0
28	22674.0
29	30755.0
30	42032.0
31	57628.0
32	79293.0
33	120993.0
34	276931.0
35	624463.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44825588878877	17.32519047006111	11.483367964253535	25.74318567689658
2	16.27856763856678	20.00571180498397	38.0805885149843	25.63513204146495
3	18.63596463368249	24.56976414036629	28.950987512503186	27.84328371344803
4	12.294479823324963	15.412234103608341	36.62747186652035	35.665814206546344
5	14.05419510052539	37.02964569333213	34.06997369214453	14.846185513997954
6	33.374399502920795	36.188069793103026	17.09367830956342	13.343852394412755
7	29.33900708351539	30.957724643547742	21.48699493509902	18.216273337837848
8	27.74828325556268	33.34379117776244	19.873220317194075	19.034705249480808
9	27.631546728128086	14.054785757355933	18.829600653792824	39.484066860723146
10	15.52283530603687	27.069196134786615	32.25913580587005	25.14883275330646
11	36.91857506556036	21.469592772022885	22.54396389378418	19.06786826863257
12	24.143878726196537	24.24022130479532	29.490930099977426	22.12496986903072
13	29.516095827245852	19.703239522960747	25.436634183935475	25.344030465857927
14	23.39164772596827	19.842581751115397	25.805343190149305	30.960427332767026
15	24.794014026563676	27.237220067645296	23.18646236838137	24.782303537409657
16	25.119213888580642	26.15955643479492	24.32981169907055	24.391417977553886
17	23.380826933586007	26.117704326315934	25.9212345070498	24.58023423304826
18	24.352400994834515	25.134340922135067	26.88854027166635	23.62471781136407
19	25.102297415914087	25.41475700011249	26.060032340293972	23.422913243679446
20	25.2264206821662	24.4699632446629	25.995810978236833	24.307805094934068
21	26.4450217907855	24.549242583671642	24.97725263919127	24.028482986351584
22	25.332335427863452	24.70213975727327	25.524952610969155	24.440572203894124
23	23.96856822093467	24.57773495583924	26.032847556024592	25.4208492672015
24	24.287220869309667	25.49979106812404	25.8718887900778	24.341099272488492
25	24.475627769571638	24.83051560909529	26.058426003168506	24.635430618164563
26	24.039535245040298	25.66339170705835	26.432345181814217	23.864727866087136
27	24.68977645538176	25.32839883708739	25.506478439511476	24.47534626801937
28	24.042073686345184	25.126133054780738	26.233303105784938	24.59849015308914
29	23.944904770323024	25.52600745551905	26.170310626219212	24.358777147938714
30	24.078696653632186	25.49935337735979	26.327948637501052	24.094001331506977
31	24.292270359495205	25.42768351075838	25.523723377634113	24.756322752112304
32	24.083612974321497	25.555831901648002	25.380050277982914	24.98050484604759
33	23.847995212447636	25.08850390519177	25.934896634479255	25.12860424788134
34	24.604736201258323	25.324128177282674	26.06173454589147	24.009401075567535
35	25.094060057614655	25.16730291777513	26.064661888915765	23.67397513569445
36	23.99524273821131	25.935112663530873	25.585894749409547	24.483749848848262
37	24.824309732203876	25.75604402836758	25.507249925958693	23.912396313469856
38	24.05365715164673	25.651219331145192	25.89985314610929	24.39527037109879
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	410.0
1	358.0
2	306.0
3	306.0
4	813.0
5	1320.0
6	1320.0
7	1497.5
8	1675.0
9	1623.5
10	1572.0
11	1572.0
12	2003.5
13	2435.0
14	3153.0
15	3871.0
16	3871.0
17	5807.0
18	7743.0
19	7743.0
20	9208.5
21	10674.0
22	11531.0
23	12388.0
24	12388.0
25	14279.5
26	16171.0
27	16171.0
28	20504.0
29	24837.0
30	29543.0
31	34249.0
32	34249.0
33	41930.0
34	49611.0
35	49611.0
36	54709.0
37	59807.0
38	67156.5
39	74506.0
40	74506.0
41	79668.5
42	84831.0
43	94150.0
44	103469.0
45	103469.0
46	107688.5
47	111908.0
48	111908.0
49	116207.5
50	120507.0
51	119439.5
52	118372.0
53	118372.0
54	112585.5
55	106799.0
56	106799.0
57	102899.5
58	99000.0
59	88677.0
60	78354.0
61	78354.0
62	72786.0
63	67218.0
64	55512.5
65	43807.0
66	43807.0
67	35974.0
68	28141.0
69	28141.0
70	22369.5
71	16598.0
72	13080.5
73	9563.0
74	9563.0
75	7339.0
76	5115.0
77	5115.0
78	4751.0
79	4387.0
80	3378.5
81	2370.0
82	2370.0
83	2339.5
84	2309.0
85	2309.0
86	1571.5
87	834.0
88	691.0
89	548.0
90	548.0
91	395.0
92	242.0
93	218.0
94	194.0
95	194.0
96	173.0
97	152.0
98	152.0
99	348.0
100	544.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12587644824871044
2	0.058920890669609136
3	0.013008508329653968
4	0.002372139754231017
5	2.2956191169977587E-4
6	2.2956191169977587E-4
7	0.0
8	2.2956191169977587E-4
9	7.652063723325862E-5
10	7.652063723325862E-5
11	0.0019895365680647242
12	0.0032138667637968622
13	0.014845003623252173
14	0.009718120928623845
15	0.024180521365709723
16	0.011631136859455311
17	0.02104317523914612
18	0.0066572954392935
19	0.00826422882119193
20	0.006733816076526759
21	0.007039898625459793
22	0.0076520637233258625
23	0.010253765389256656
24	0.014615441711552397
25	0.01737018465194971
26	0.019589283131714208
27	0.009029435193524518
28	0.006274692253127208
29	0.011784178133921828
30	0.0035964699499631556
31	0.006733816076526759
32	0.007805104997792379
33	0.008799873281824742
34	0.013620673427520033
35	0.01721714337748319
36	0.0155336893583515
37	0.010253765389256656
38	0.007345981174392828
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1306837.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.8874293990463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98041980963082	44.140126360177796
2	12.670076691336412	14.161960331351315
3	3.814890663885696	6.39613297928978
4	1.569567762468695	3.5087643004795304
5	0.796541317459421	2.2258323321468354
6	0.45726411246732096	1.5333189481340976
7	0.31702097650415845	1.240224120967501
8	0.23575094759733062	1.0540411551683262
9	0.16075311964203473	0.8085670762203592
>10	0.8371076916394004	8.51260906911173
>50	0.07691850968919615	3.0090542728527097
>100	0.07351938219967999	8.384881463315335
>500	0.007558052045858772	2.811111066222846
>1k	0.002610963434023939	2.213376524561987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4362	0.3337830196114741	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3059	0.23407662929653814	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1793	0.13720150255923272	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1394	0.10666976830316253	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1355	0.10368546345106544	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1349	0.10322633962766589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.5304127446651725E-4
6	0.0	0.0	0.0	0.0	1.5304127446651725E-4
7	0.0	0.0	0.0	0.0	1.5304127446651725E-4
8	0.0	0.0	0.0	0.0	1.5304127446651725E-4
9	0.0	0.0	0.0	0.0	1.5304127446651725E-4
10	0.0	0.0	0.0	0.0	1.5304127446651725E-4
11	0.0	0.0	0.0	0.0	1.5304127446651725E-4
12	0.0	0.0	0.0	0.0	2.2956191169977587E-4
13	0.0	0.0	0.0	0.0	3.060825489330345E-4
14	0.0	0.0	0.0	0.0	3.060825489330345E-4
15	0.0	0.0	0.0	0.0	3.826031861662931E-4
16	0.0	0.0	0.0	0.0	3.826031861662931E-4
17	0.0	0.0	0.0	0.0	3.826031861662931E-4
18	0.0	0.0	0.0	0.0	3.826031861662931E-4
19	0.0	0.0	0.0	0.0	3.826031861662931E-4
20	0.0	0.0	0.0	1.5304127446651725E-4	4.5912382339955175E-4
21	0.0	0.0	0.0	4.5912382339955175E-4	5.356444606328104E-4
22	0.0	0.0	0.0	6.12165097866069E-4	5.356444606328104E-4
23	0.0	0.0	0.0	0.0010712889212656207	5.356444606328104E-4
24	0.0	0.0	0.0	0.0021425778425312415	5.356444606328104E-4
25	0.0	0.0	0.0	0.0029077842148638277	5.356444606328104E-4
26	0.0	0.0	0.0	0.004208635047829225	6.12165097866069E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCCG	360	0.0	16.44468	15
GTCTTAC	90	8.503805E-6	16.015554	1
GTATAGG	110	1.997596E-7	16.015553	1
GTCCTAT	430	0.0	15.643097	1
TAGCGTA	215	0.0	15.626935	7
ACCGCAG	385	0.0	15.376256	29
ATAGCGT	220	0.0	15.269441	6
AATAGCG	230	0.0	14.605552	5
GTATAGA	110	3.5075773E-6	14.559593	1
TGTAGGA	275	0.0	14.552347	2
ACGAACG	155	3.1322998E-9	14.451821	15
CTAGCGG	310	0.0	14.451268	29
ACCGTCG	180	1.1823431E-10	14.221337	8
AGGCCCG	440	0.0	14.182021	10
CGACCCG	205	3.6379788E-12	14.045756	5
CCGATAA	160	4.8967195E-9	14.0002	9
TCCGATA	160	4.9021764E-9	13.999129	8
GTCTTAA	195	2.7284841E-11	13.962276	1
GTAGGAC	275	0.0	13.961166	3
GTATTAG	255	0.0	13.81734	1
>>END_MODULE