FastQCFastQC Report
Thu 2 Feb 2017
SRR4062823_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062823_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306837
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54100.4139766474319292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39190.29988437731714057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24050.184032132545987No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.12916683564974055No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14930.11424531138925513No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14440.11049580016482545No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA13580.1039150253627652No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13500.10330286026489913No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13160.10070115859896836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC3150.018.288367
GTATAGG1055.971742E-918.287661
CGGTCCA3150.018.2876610
ACCGTCG3150.017.7803528
TTGCGCA450.00885133317.777634
TCTAGCG3100.017.5475728
ATACCGT3250.017.2332616
GTATTAC753.231287E-517.0684831
TAGCGGC3200.016.99920730
CGTCGTA3450.016.6974310
CGAACGA1151.8424544E-816.69487416
ACGAACG1151.8424544E-816.69487415
CTAGCGG3300.016.4840829
ATCGTTT2450.016.3257729
CGCCGGT3250.016.2485037
CCGTCGT3350.016.2411569
ATTATAC1703.6379788E-1215.9998663
CATCGTT2500.015.99925428
GCGATTT2750.015.70835927
AACCGCG2700.015.40963657
Produced by FastQC (version 0.11.5)