##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1306837
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16330345712587	32.0	32.0	32.0	32.0	32.0
2	31.29737756124138	32.0	32.0	32.0	32.0	32.0
3	31.383959131858067	32.0	32.0	32.0	32.0	32.0
4	31.486336092412444	32.0	32.0	32.0	32.0	32.0
5	31.4047620322963	32.0	32.0	32.0	32.0	32.0
6	34.94061768988787	36.0	36.0	36.0	36.0	36.0
7	34.96466430013842	36.0	36.0	36.0	36.0	36.0
8	34.902488221560915	36.0	36.0	36.0	36.0	36.0
9	35.01828537147326	36.0	36.0	36.0	36.0	36.0
10	34.86722062506648	36.0	36.0	36.0	32.0	36.0
11	35.03375707911545	36.0	36.0	36.0	36.0	36.0
12	34.93737780610742	36.0	36.0	36.0	36.0	36.0
13	34.984799940619986	36.0	36.0	36.0	36.0	36.0
14	34.94048530918546	36.0	36.0	36.0	32.0	36.0
15	34.91625428419918	36.0	36.0	36.0	32.0	36.0
16	34.93061185136325	36.0	36.0	36.0	36.0	36.0
17	34.89516443137132	36.0	36.0	36.0	32.0	36.0
18	34.88771438213029	36.0	36.0	36.0	32.0	36.0
19	34.88193324798731	36.0	36.0	36.0	32.0	36.0
20	34.86408710497177	36.0	36.0	36.0	32.0	36.0
21	34.85759509410891	36.0	36.0	36.0	32.0	36.0
22	34.84321533596003	36.0	36.0	36.0	32.0	36.0
23	34.78386975575378	36.0	36.0	36.0	32.0	36.0
24	34.762515906727465	36.0	36.0	36.0	32.0	36.0
25	34.74964819637032	36.0	36.0	36.0	32.0	36.0
26	34.68575346428055	36.0	36.0	36.0	32.0	36.0
27	34.67997615616944	36.0	36.0	36.0	32.0	36.0
28	34.641681403266055	36.0	36.0	36.0	32.0	36.0
29	34.60918691466495	36.0	36.0	36.0	32.0	36.0
30	34.58770298055534	36.0	36.0	36.0	32.0	36.0
31	34.571444640762394	36.0	36.0	36.0	32.0	36.0
32	34.55236575028102	36.0	36.0	36.0	32.0	36.0
33	34.502833941799935	36.0	36.0	36.0	32.0	36.0
34	34.488643954831396	36.0	36.0	36.0	32.0	36.0
35	34.446093889291475	36.0	36.0	36.0	32.0	36.0
36	34.42184296893951	36.0	36.0	36.0	32.0	36.0
37	34.408852060356416	36.0	36.0	36.0	32.0	36.0
38	33.986748156043944	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	10.0
21	34.0
22	86.0
23	309.0
24	834.0
25	1975.0
26	4399.0
27	8405.0
28	15018.0
29	23429.0
30	35432.0
31	51572.0
32	74074.0
33	118637.0
34	292729.0
35	679894.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90640465715329	18.48902711443376	12.116434954362868	26.488133274050085
2	15.354095422765043	21.008740952391154	37.34796305889717	26.289200565946636
3	17.679710629558237	25.206892672919423	29.262027322458728	27.851369375063605
4	11.876183203069402	16.325256306462794	36.677190829133615	35.121369661334185
5	13.57690362302261	37.47146736739165	33.8616828265499	15.089946183035835
6	33.499638439435735	36.57799186584381	16.671653269157925	13.250716425562523
7	29.139629271398316	31.147232391003588	21.470242540739743	18.242895796858356
8	27.491108104633582	33.5828981763197	19.82242226861176	19.103571450434952
9	27.376597815706344	13.831742659842789	18.827518549354753	39.964140975096115
10	15.260020507797552	27.176734362804368	32.434535743254614	25.12870938614346
11	37.13856299879932	21.36370558395057	22.458777911526422	19.038953505723686
12	24.025331546820585	24.242651486919865	29.467971708616815	22.264045257642735
13	29.521446122037016	19.71476079958189	25.46637558127167	25.297417497109425
14	23.233195876761982	19.779896039062255	25.9509028287384	31.036005255437367
15	24.713793686588303	27.28618794845876	23.074492075140203	24.92552628981273
16	25.00503123189809	26.160569374757525	24.352692799484558	24.48170659385983
17	23.436970333714154	26.13478192000992	25.876524769347668	24.55172297692826
18	24.308081267977567	24.96095534485173	27.122892908602985	23.608070478567715
19	25.10557934922259	25.252269410798746	26.244435993165176	23.39771524681349
20	25.125398194266	24.450333132594196	26.172812676714845	24.251455996424955
21	26.301580766952803	24.510075495434002	25.230978073726273	23.95736566388692
22	25.208652647575786	24.602762241962846	25.851196438423464	24.337388672037903
23	23.96212829030182	24.297519269868452	26.282088070931785	25.458264368897936
24	24.2389066119187	25.318000638182113	26.073794972135012	24.369297777764174
25	24.422804391675772	24.598495909203603	26.239176147580874	24.73952355153975
26	24.069489921084266	25.441811029225526	26.570490428416093	23.918208621274115
27	24.821783299511186	25.018824091163694	25.685319351471797	24.47407325785332
28	23.888289052115912	24.903947470112954	26.633466912859067	24.574296564912075
29	23.96389144170237	25.186614704052605	26.36679249210116	24.482701362143864
30	24.052502339618485	25.252728534622143	26.66193258990984	24.032836535849537
31	24.234391894321938	25.090045659864234	25.738481539778867	24.937080906034954
32	24.134532462732537	25.271476090744294	25.719810504293957	24.874180942229216
33	23.887600366380813	24.731776036338122	26.267621746246856	25.113001851034216
34	24.5124678900276	24.992558368029066	26.376816695578714	24.11815704636462
35	25.16595413199963	24.838216242729583	26.261500095268193	23.734329530002594
36	24.159095587284412	25.52759066356401	25.822424678823758	24.490889070327825
37	24.860560268801695	25.401637694678065	25.758376905459517	23.979425131060722
38	24.146775764689856	25.205056177625824	26.160339812845823	24.487828244838493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	182.5
2	195.0
3	195.0
4	744.0
5	1293.0
6	1293.0
7	1552.5
8	1812.0
9	1771.5
10	1731.0
11	1731.0
12	2184.5
13	2638.0
14	3477.5
15	4317.0
16	4317.0
17	6470.0
18	8623.0
19	8623.0
20	10205.0
21	11787.0
22	12289.5
23	12792.0
24	12792.0
25	14477.5
26	16163.0
27	16163.0
28	20461.5
29	24760.0
30	29158.0
31	33556.0
32	33556.0
33	41666.0
34	49776.0
35	49776.0
36	54626.5
37	59477.0
38	66746.5
39	74016.0
40	74016.0
41	79211.5
42	84407.0
43	93585.0
44	102763.0
45	102763.0
46	107428.5
47	112094.0
48	112094.0
49	116770.0
50	121446.0
51	120032.0
52	118618.0
53	118618.0
54	112443.0
55	106268.0
56	106268.0
57	102614.0
58	98960.0
59	88792.0
60	78624.0
61	78624.0
62	73057.5
63	67491.0
64	55762.5
65	44034.0
66	44034.0
67	36211.0
68	28388.0
69	28388.0
70	22619.0
71	16850.0
72	13266.5
73	9683.0
74	9683.0
75	7213.5
76	4744.0
77	4744.0
78	4440.0
79	4136.0
80	3232.0
81	2328.0
82	2328.0
83	2110.0
84	1892.0
85	1892.0
86	1203.0
87	514.0
88	442.0
89	370.0
90	370.0
91	226.5
92	83.0
93	51.5
94	20.0
95	20.0
96	14.5
97	9.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008876393919058001
2	0.0
3	0.0
4	2.2956191169977587E-4
5	0.0
6	1.5304127446651725E-4
7	3.8260318616629307E-4
8	0.0022190984797645003
9	0.004055593773362707
10	0.0013008508329653966
11	0.005662527155261139
12	6.12165097866069E-4
13	3.060825489330345E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.2956191169977587E-4
22	0.0
23	3.060825489330345E-4
24	0.0
25	7.652063723325862E-5
26	0.0
27	7.652063723325862E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1306837.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.14489932587888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64757108755582	42.04219919434734
2	13.51090800712117	14.63093507693573
3	4.061285546601307	6.596936911631243
4	1.6239138527835193	3.51706608291455
5	0.8356555446889745	2.2623242669148507
6	0.5159337996200745	1.676111018354826
7	0.33048904795324147	1.2526007360813671
8	0.23711064268749077	1.0270665501926903
9	0.16203129326980348	0.7895851255561918
>10	0.9048483463969409	8.856862348265008
>50	0.08225840309221893	3.145570247695356
>100	0.07662198105832689	8.569923835554073
>500	0.00838717978866381	3.0043700449659467
>1k	0.0028431117927673927	2.2120408557445326
>5k	1.4215558963836966E-4	0.4164077048463037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5410	0.4139766474319292	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3919	0.29988437731714057	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2405	0.184032132545987	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.12916683564974055	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1493	0.11424531138925513	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1444	0.11049580016482545	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1358	0.1039150253627652	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1350	0.10330286026489913	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1316	0.10070115859896836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.652063723325862E-5
2	0.0	0.0	0.0	0.0	7.652063723325862E-5
3	0.0	0.0	0.0	0.0	7.652063723325862E-5
4	0.0	0.0	0.0	0.0	7.652063723325862E-5
5	0.0	0.0	0.0	0.0	7.652063723325862E-5
6	0.0	0.0	0.0	0.0	7.652063723325862E-5
7	0.0	0.0	0.0	0.0	7.652063723325862E-5
8	0.0	0.0	0.0	0.0	7.652063723325862E-5
9	0.0	0.0	0.0	0.0	7.652063723325862E-5
10	0.0	0.0	0.0	0.0	7.652063723325862E-5
11	0.0	0.0	0.0	0.0	7.652063723325862E-5
12	0.0	0.0	0.0	0.0	1.5304127446651725E-4
13	0.0	0.0	0.0	0.0	1.5304127446651725E-4
14	0.0	0.0	0.0	0.0	1.5304127446651725E-4
15	0.0	0.0	0.0	0.0	1.5304127446651725E-4
16	0.0	0.0	0.0	0.0	1.5304127446651725E-4
17	0.0	0.0	0.0	0.0	1.5304127446651725E-4
18	0.0	0.0	0.0	0.0	2.2956191169977587E-4
19	0.0	0.0	0.0	0.0	2.2956191169977587E-4
20	0.0	0.0	0.0	1.5304127446651725E-4	3.060825489330345E-4
21	0.0	0.0	0.0	4.5912382339955175E-4	3.060825489330345E-4
22	0.0	0.0	0.0	6.12165097866069E-4	3.060825489330345E-4
23	0.0	0.0	0.0	9.947682840323621E-4	3.060825489330345E-4
24	0.0	0.0	0.0	0.0020660572052979827	3.060825489330345E-4
25	0.0	0.0	0.0	0.0026782223031640517	3.060825489330345E-4
26	0.0	0.0	0.0	0.0039025524988961897	3.060825489330345E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGTC	315	0.0	18.28836	7
GTATAGG	105	5.971742E-9	18.28766	1
CGGTCCA	315	0.0	18.28766	10
ACCGTCG	315	0.0	17.780352	8
TTGCGCA	45	0.008851333	17.77763	4
TCTAGCG	310	0.0	17.54757	28
ATACCGT	325	0.0	17.233261	6
GTATTAC	75	3.231287E-5	17.068483	1
TAGCGGC	320	0.0	16.999207	30
CGTCGTA	345	0.0	16.69743	10
CGAACGA	115	1.8424544E-8	16.694874	16
ACGAACG	115	1.8424544E-8	16.694874	15
CTAGCGG	330	0.0	16.48408	29
ATCGTTT	245	0.0	16.32577	29
CGCCGGT	325	0.0	16.248503	7
CCGTCGT	335	0.0	16.241156	9
ATTATAC	170	3.6379788E-12	15.999866	3
CATCGTT	250	0.0	15.999254	28
GCGATTT	275	0.0	15.708359	27
AACCGCG	270	0.0	15.4096365	7
>>END_MODULE