FastQCFastQC Report
Thu 2 Feb 2017
SRR4062822_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062822_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617333
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36360.22481455581503623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24740.15296787983674356No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19600.12118716430073462No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18940.11710637203346498No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18400.113767541996608No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18080.1117889760488409No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17930.11086152326082507No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17050.10542046690446556No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17040.10535863671859785No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16970.1049258254175238No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16940.10474033485992063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAT957.594863E-716.8636631
ACGAACG1950.016.40941615
TCTAGCG3450.015.76779628
GTCCTAC5350.014.972411
TACGCTA4400.014.9083289
CTAGCGG3650.014.90380829
GTTTTCG4550.014.76893128
TAACGAA1951.8189894E-1214.76847413
ACGCTAT4600.014.60794710
GTACTAC1103.4976438E-614.5640731
TCCGATA2200.014.544718
CGAACGA2003.6379788E-1214.39926216
AACGCTT3900.014.35868330
ATACGCT4600.014.26013858
CCGATAA2053.6379788E-1214.0480619
AAACGCT4000.013.99971629
TAGGACG4700.013.9554384
AACGAAC2107.2759576E-1213.71358314
CGATAAC2107.2759576E-1213.71358310
GGCTAGT700.00680767613.71358326
Produced by FastQC (version 0.11.5)