##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062822_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1617333
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29103901299238	32.0	32.0	32.0	32.0	32.0
2	30.97039818021397	32.0	32.0	32.0	32.0	32.0
3	31.012371601890273	32.0	32.0	32.0	32.0	32.0
4	31.067736205221806	32.0	32.0	32.0	32.0	32.0
5	31.021309155257452	32.0	32.0	32.0	32.0	32.0
6	34.669994985571925	36.0	36.0	36.0	32.0	36.0
7	34.61138491578419	36.0	36.0	36.0	32.0	36.0
8	34.603802680091235	36.0	36.0	36.0	32.0	36.0
9	34.69535339970186	36.0	36.0	36.0	32.0	36.0
10	34.473550592240436	36.0	36.0	36.0	32.0	36.0
11	34.68185772503251	36.0	36.0	36.0	32.0	36.0
12	34.52819858371776	36.0	36.0	36.0	32.0	36.0
13	34.596449216085986	36.0	36.0	36.0	32.0	36.0
14	34.518253816622796	36.0	36.0	36.0	32.0	36.0
15	34.47173649458708	36.0	36.0	36.0	32.0	36.0
16	34.487591609149135	36.0	36.0	36.0	32.0	36.0
17	34.429870039132325	36.0	36.0	36.0	32.0	36.0
18	34.44927482466505	36.0	36.0	36.0	32.0	36.0
19	34.430062331010376	36.0	36.0	36.0	32.0	36.0
20	34.40505449403432	36.0	36.0	36.0	32.0	36.0
21	34.39103264448323	36.0	36.0	36.0	32.0	36.0
22	34.372484207024776	36.0	36.0	36.0	32.0	36.0
23	34.31665278578994	36.0	36.0	36.0	32.0	36.0
24	34.30321337658973	36.0	36.0	36.0	32.0	36.0
25	34.28595842661963	36.0	36.0	36.0	32.0	36.0
26	34.24197985201563	36.0	36.0	36.0	32.0	36.0
27	34.24955404978443	36.0	36.0	36.0	32.0	36.0
28	34.2223716451714	36.0	36.0	36.0	32.0	36.0
29	34.18366842202564	36.0	36.0	36.0	32.0	36.0
30	34.16285576316071	36.0	36.0	36.0	32.0	36.0
31	34.17875601375845	36.0	36.0	36.0	32.0	36.0
32	34.139398008944355	36.0	36.0	36.0	32.0	36.0
33	34.10758390510798	36.0	36.0	36.0	32.0	36.0
34	34.11145756625259	36.0	36.0	36.0	32.0	36.0
35	34.07088645319177	36.0	36.0	36.0	32.0	36.0
36	34.033895926194546	36.0	36.0	36.0	32.0	36.0
37	34.02486377264299	36.0	36.0	36.0	32.0	36.0
38	33.446088096885426	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	5.0
8	1.0
9	5.0
10	21.0
11	17.0
12	9.0
13	9.0
14	239.0
15	461.0
16	571.0
17	641.0
18	865.0
19	1022.0
20	1365.0
21	1909.0
22	2798.0
23	4137.0
24	5929.0
25	9151.0
26	13489.0
27	19546.0
28	28110.0
29	38108.0
30	52004.0
31	70197.0
32	96775.0
33	147046.0
34	339606.0
35	783296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01839513226976	17.351534289603702	11.509787522757902	26.12028305536864
2	16.594709058863778	19.74442934286692	37.83425644045851	25.8266051578108
3	18.560075292767415	23.79569292720421	28.867813454995627	28.776418325032743
4	12.560718631592485	15.487393773310512	36.144403992837425	35.80748360225957
5	14.643764723340318	36.51400765459121	33.70425330637532	15.137974315693148
6	34.04493958240974	35.64523438982964	16.893058732093138	13.416767295667482
7	30.13926639675713	30.524839674228915	21.130046273739715	18.20584765527424
8	27.96027024837896	33.15068628669403	19.642595467706904	19.246447997220102
9	27.699531387282843	14.20677451127694	18.754216061439646	39.33947804000057
10	16.09461060368856	26.452028165042996	31.64665830717902	25.80670292408942
11	37.40098448213284	20.990887882683268	22.551578343371688	19.056549291812203
12	24.833192046641305	23.735669603080236	28.89600371982818	22.535134630450283
13	29.63764360743611	19.44558160934325	25.25468714635374	25.662087636866897
14	23.542080971179246	20.051720043928643	25.10487469452772	31.30132429036439
15	25.130987838787245	27.54480056204791	22.292732463214556	25.031479135950285
16	25.65757904058018	25.650282225948956	24.145221452140316	24.546917281330543
17	23.87321644139241	26.013857829847026	25.50055906275356	24.612366666007006
18	24.908315282888548	24.719659021275305	26.579880251813755	23.792145444022392
19	25.648036844110038	24.930063962720567	25.379484934380088	24.042414258789307
20	25.717877386870043	24.458504124019235	25.09694026772889	24.726678221381828
21	26.940809244551733	24.247841346712736	24.24338924024803	24.567960168487495
22	25.81495291273242	24.617705801967585	24.884956189981512	24.68238509531848
23	24.207619320880163	24.287512862118092	25.665243588728824	25.83962422827291
24	24.805236085155645	25.06681772026967	25.313805202691757	24.81414099188293
25	24.82585507877337	24.573604676186918	25.486814211610813	25.1137260334289
26	24.410674463735095	25.49476876571566	25.86458530455443	24.22997146599481
27	25.14995312805317	24.947748291461885	24.985035605365415	24.91726297511953
28	24.264420353840027	24.914235964254885	25.651608802222075	25.169734879683013
29	24.369658000739577	25.134527789217742	25.553292297874393	24.942521912168285
30	24.21253294123321	25.239940789254018	26.008270707375498	24.539255562137274
31	25.16924104327179	24.834840034132647	24.702019514970132	25.29389940762543
32	24.500976682667485	25.1471516668532	24.728343708203454	25.62352794227586
33	24.099100352525276	24.70471911446343	25.651176207604674	25.545004325406616
34	25.04631679450679	24.831923835154264	25.74805642609967	24.373702944239277
35	25.52814919897938	24.870784175251163	25.513369308041373	24.087697317728075
36	24.28250214275378	25.447566536679123	25.2139970984702	25.055934222096894
37	25.295996388742026	25.201819233040535	24.85454483617936	24.64763954203807
38	24.53285200966852	25.2462424414438	25.279509673158724	24.941395875728958
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	690.0
1	515.5
2	341.0
3	341.0
4	697.0
5	1053.0
6	1053.0
7	1196.0
8	1339.0
9	1227.5
10	1116.0
11	1116.0
12	1566.5
13	2017.0
14	2655.0
15	3293.0
16	3293.0
17	4979.5
18	6666.0
19	6666.0
20	8133.0
21	9600.0
22	10851.5
23	12103.0
24	12103.0
25	14484.5
26	16866.0
27	16866.0
28	21464.5
29	26063.0
30	32713.5
31	39364.0
32	39364.0
33	49978.5
34	60593.0
35	60593.0
36	67539.5
37	74486.0
38	83386.5
39	92287.0
40	92287.0
41	97236.0
42	102185.0
43	114051.5
44	125918.0
45	125918.0
46	129890.5
47	133863.0
48	133863.0
49	141360.0
50	148857.0
51	150182.5
52	151508.0
53	151508.0
54	143075.5
55	134643.0
56	134643.0
57	130721.0
58	126799.0
59	113286.5
60	99774.0
61	99774.0
62	93954.5
63	88135.0
64	72875.5
65	57616.0
66	57616.0
67	48048.5
68	38481.0
69	38481.0
70	31116.0
71	23751.0
72	18631.0
73	13511.0
74	13511.0
75	10254.0
76	6997.0
77	6997.0
78	6741.5
79	6486.0
80	5018.5
81	3551.0
82	3551.0
83	3666.0
84	3781.0
85	3781.0
86	2490.0
87	1199.0
88	1061.5
89	924.0
90	924.0
91	645.5
92	367.0
93	317.0
94	267.0
95	267.0
96	240.5
97	214.0
98	214.0
99	421.5
100	629.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12118716430073462
2	0.053915922076653355
3	0.01106760327032219
4	0.0022877168771057044
5	1.854905576031652E-4
6	3.709811152063304E-4
7	6.183018586772174E-5
8	3.709811152063304E-4
9	3.709811152063304E-4
10	5.564716728094956E-4
11	0.0022258866912379825
12	0.0033388300368569737
13	0.015519376652798155
14	0.010201980668174087
15	0.024051942302543757
16	0.011685905128999408
17	0.021084093380893114
18	0.006553999701978504
19	0.008718056207348765
20	0.006183018586772174
21	0.00748145248999433
22	0.007605112861729774
23	0.011191263642057634
24	0.01527205590932727
25	0.017868923715771583
26	0.01947650854833235
27	0.0099546599247032
28	0.006492169516110782
29	0.012056886244205738
30	0.003647980966195582
31	0.006986811003052556
32	0.007666943047597495
33	0.00890354676495193
34	0.012922508846353842
35	0.016755980370152593
36	0.015766697396269045
37	0.010387471225777252
38	0.007790603419332938
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1617333.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.79492402215984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8406320867062	45.10496925181149
2	11.784619218573248	13.150438678607582
3	3.297334312974814	5.519235524042711
4	1.3573113495902156	3.029243344992053
5	0.7062364898857838	1.970220564742708
6	0.4172372352513211	1.3967831904038097
7	0.2960101902379919	1.156110625187969
8	0.20331814271678053	0.9075296260167539
9	0.14696908910092413	0.7380116243992891
>10	0.7903405454367659	7.9160549540388185
>50	0.0698859500434783	2.7570174396265674
>100	0.07618324545805454	9.262151989305691
>500	0.009912566545346222	3.786919933512468
>1k	0.004009577479016449	3.3053132533122405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3636	0.22481455581503623	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2474	0.15296787983674356	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1960	0.12118716430073462	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1894	0.11710637203346498	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1840	0.113767541996608	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1808	0.1117889760488409	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1793	0.11086152326082507	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1705	0.10542046690446556	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1704	0.10535863671859785	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1697	0.1049258254175238	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1694	0.10474033485992063	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.183018586772174E-5
7	0.0	0.0	0.0	0.0	6.183018586772174E-5
8	0.0	0.0	0.0	0.0	6.183018586772174E-5
9	0.0	0.0	6.183018586772174E-5	1.2366037173544347E-4	6.183018586772174E-5
10	0.0	0.0	6.183018586772174E-5	1.2366037173544347E-4	6.183018586772174E-5
11	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	6.183018586772174E-5
12	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	1.2366037173544347E-4
13	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	1.2366037173544347E-4
14	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	1.2366037173544347E-4
15	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	1.2366037173544347E-4
16	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	2.4732074347088694E-4
17	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	3.709811152063304E-4
18	0.0	0.0	6.183018586772174E-5	2.4732074347088694E-4	3.709811152063304E-4
19	0.0	0.0	6.183018586772174E-5	3.709811152063304E-4	3.709811152063304E-4
20	0.0	0.0	6.183018586772174E-5	4.946414869417739E-4	4.3281130107405215E-4
21	0.0	0.0	6.183018586772174E-5	6.183018586772174E-4	4.3281130107405215E-4
22	0.0	0.0	6.183018586772174E-5	8.037924162803826E-4	4.3281130107405215E-4
23	0.0	0.0	6.183018586772174E-5	0.0012366037173544347	5.564716728094956E-4
24	0.0	0.0	6.183018586772174E-5	0.0014839244608253217	5.564716728094956E-4
25	0.0	0.0	6.183018586772174E-5	0.0019785659477670955	5.564716728094956E-4
26	0.0	0.0	6.183018586772174E-5	0.002411377248841148	5.564716728094956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAT	95	7.594863E-7	16.863663	1
ACGAACG	195	0.0	16.409416	15
TCTAGCG	345	0.0	15.767796	28
GTCCTAC	535	0.0	14.97241	1
TACGCTA	440	0.0	14.908328	9
CTAGCGG	365	0.0	14.903808	29
GTTTTCG	455	0.0	14.768931	28
TAACGAA	195	1.8189894E-12	14.768474	13
ACGCTAT	460	0.0	14.607947	10
GTACTAC	110	3.4976438E-6	14.564073	1
TCCGATA	220	0.0	14.54471	8
CGAACGA	200	3.6379788E-12	14.399262	16
AACGCTT	390	0.0	14.358683	30
ATACGCT	460	0.0	14.2601385	8
CCGATAA	205	3.6379788E-12	14.048061	9
AAACGCT	400	0.0	13.999716	29
TAGGACG	470	0.0	13.955438	4
AACGAAC	210	7.2759576E-12	13.713583	14
CGATAAC	210	7.2759576E-12	13.713583	10
GGCTAGT	70	0.006807676	13.713583	26
>>END_MODULE