FastQCFastQC Report
Thu 2 Feb 2017
SRR4062822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1617333
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42780.26450953514211356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33440.20676014154166147No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG20610.1274320130733745No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19080.11797199463561307No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18900.11685905128999408No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18550.11469499478462383No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18170.11234544772165041No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18070.11172714586297318No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17560.10857380638371937No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16960.10486399523165607No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16600.10263810854041808No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16240.1004122218491801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3850.020.3634429
CATCGTT4100.019.12176728
CGGCATC4350.018.75843825
CGTTTAT4400.017.45437631
AGGGGCG3600.016.88872532
TCGTTTA4550.016.87895830
GGCATCG4850.016.8245826
TCTAGCG2900.016.55156528
CTAGCGG3000.015.999845529
GCATCGT5250.015.847465527
GTAGGAC7050.015.4329523
CGCATCG5450.015.26590713
CGTACAT951.4332403E-515.15774826
TAACCCG1807.2759576E-1215.11096628
GTCCTAC5250.014.9345751
TAAACGC3750.014.93318928
GCATCGC5800.014.89640914
CAATTGG650.00416025514.7704571
TAGCGGC3150.014.73001730
ACCGTCG3600.014.6669798
Produced by FastQC (version 0.11.5)