##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1617333
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.226467894985138	32.0	32.0	32.0	32.0	32.0
2	31.321894130645948	32.0	32.0	32.0	32.0	32.0
3	31.40007345426081	32.0	32.0	32.0	32.0	32.0
4	31.49015508865521	32.0	32.0	32.0	32.0	32.0
5	31.424820986154366	32.0	32.0	32.0	32.0	32.0
6	34.96053502896435	36.0	36.0	36.0	36.0	36.0
7	34.98172794347237	36.0	36.0	36.0	36.0	36.0
8	34.91460880350552	36.0	36.0	36.0	36.0	36.0
9	35.02396043362746	36.0	36.0	36.0	36.0	36.0
10	34.880721533537	36.0	36.0	36.0	32.0	36.0
11	35.03844724617627	36.0	36.0	36.0	36.0	36.0
12	34.94754883502655	36.0	36.0	36.0	36.0	36.0
13	34.99201648640076	36.0	36.0	36.0	36.0	36.0
14	34.94354965860463	36.0	36.0	36.0	32.0	36.0
15	34.91133922327684	36.0	36.0	36.0	32.0	36.0
16	34.928542235890816	36.0	36.0	36.0	36.0	36.0
17	34.89192701812181	36.0	36.0	36.0	32.0	36.0
18	34.892505748661534	36.0	36.0	36.0	32.0	36.0
19	34.88500945692693	36.0	36.0	36.0	32.0	36.0
20	34.87567557206834	36.0	36.0	36.0	32.0	36.0
21	34.863874044491766	36.0	36.0	36.0	32.0	36.0
22	34.8474803890108	36.0	36.0	36.0	32.0	36.0
23	34.78918812637843	36.0	36.0	36.0	32.0	36.0
24	34.75779941422082	36.0	36.0	36.0	32.0	36.0
25	34.752490056160354	36.0	36.0	36.0	32.0	36.0
26	34.68836473379323	36.0	36.0	36.0	32.0	36.0
27	34.67295108675826	36.0	36.0	36.0	32.0	36.0
28	34.63772148345455	36.0	36.0	36.0	32.0	36.0
29	34.60651949845826	36.0	36.0	36.0	32.0	36.0
30	34.581200655647294	36.0	36.0	36.0	32.0	36.0
31	34.57606256720168	36.0	36.0	36.0	32.0	36.0
32	34.54767014585123	36.0	36.0	36.0	32.0	36.0
33	34.52305060244242	36.0	36.0	36.0	32.0	36.0
34	34.49962190841342	36.0	36.0	36.0	32.0	36.0
35	34.47849886201543	36.0	36.0	36.0	32.0	36.0
36	34.44970516275869	36.0	36.0	36.0	32.0	36.0
37	34.436432076758464	36.0	36.0	36.0	32.0	36.0
38	34.015260308174014	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	8.0
21	36.0
22	109.0
23	338.0
24	927.0
25	2428.0
26	5393.0
27	10094.0
28	18101.0
29	28821.0
30	43685.0
31	63400.0
32	91381.0
33	146420.0
34	359728.0
35	846460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.48739938522728	18.525423529680932	12.176358519722259	26.81081856536953
2	15.580032065134391	20.759732225831044	37.11622776509228	26.544007943942283
3	17.640275688432748	24.55975361907535	29.26280487691774	28.537165815574156
4	12.094424645032683	16.31501757214969	36.17544202427207	35.415115758545554
5	14.085048709850545	37.02134132014948	33.45522131510413	15.43838865489584
6	33.93955844536462	36.14046846316555	16.58547322718726	13.334499864282574
7	29.83631025988544	30.775513686772253	21.082303651454744	18.30587240188756
8	27.82931079043988	33.37917098971002	19.45789963779183	19.333618582058268
9	27.502790204450108	14.016255839333692	18.564737380701366	39.91621657551484
10	15.827480028689436	26.80718719857542	31.670232236044814	25.69510053669033
11	37.52148717200373	20.886211357098865	22.506004041443344	19.086297429454056
12	24.726292209305846	23.881008602465673	28.78403687071322	22.608662317515257
13	29.630774633779584	19.395152015788966	25.29622860365095	25.677844746780497
14	23.424056765057042	19.953342941744218	25.103302783038494	31.519297510160243
15	25.096006821106105	27.639700667704176	22.17026425603138	25.094028255158342
16	25.567880395466602	25.696487420625353	23.977234083334878	24.758398100573167
17	23.880425366946696	26.015236194401524	25.45066476724336	24.653673671408423
18	24.924428055323176	24.591225183682024	26.618513317912885	23.865833443081915
19	25.67609762491707	24.8730471708671	25.480714237575068	23.97014096664076
20	25.767235318886094	24.321954724228096	25.156538573070602	24.754271383815208
21	26.81216546005059	24.188587013311423	24.45587890681758	24.54336861982041
22	25.764546651242075	24.562751842387243	24.929034316413894	24.743667189956785
23	24.103105672246436	24.135690220492897	25.82235794651806	25.938846160742607
24	24.870450303060657	25.01099031553799	25.34444050792261	24.774118873478745
25	24.879956471468407	24.54755677567349	25.46678785405576	25.10569889880235
26	24.461706069007477	25.369188268085956	25.872053480670637	24.29705218223593
27	25.24744455683805	24.82798831656085	24.99319867534928	24.931368451251814
28	24.2637106891407	24.768801477494122	25.785660714274673	25.181827119090507
29	24.499840168969534	24.861794077039175	25.65136555056998	24.98700020342131
30	24.415132814330754	24.9726556003	26.042688796926793	24.569522788442455
31	25.12401589530418	24.684588764342287	24.810660513326567	25.380734827026963
32	24.561175713350313	24.973892204017353	24.93463003599135	25.530302046640983
33	24.214184710260657	24.57026475067287	25.700582378520686	25.514968160545788
34	24.928199696661107	24.694296103523516	25.864556031441886	24.512948168373487
35	25.6095683449234	24.694048782780044	25.55466313987286	24.141719732423688
36	24.429910228753137	25.2695641528368	25.30418905692272	24.996336561487336
37	25.412824693492308	24.99868610855031	24.92739590424483	24.66109329371255
38	24.628276692726043	24.93687134119649	25.478504104290277	24.95634786178719
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	321.0
1	245.5
2	170.0
3	170.0
4	580.5
5	991.0
6	991.0
7	1158.5
8	1326.0
9	1328.0
10	1330.0
11	1330.0
12	1669.5
13	2009.0
14	2747.0
15	3485.0
16	3485.0
17	5512.5
18	7540.0
19	7540.0
20	8855.0
21	10170.0
22	11075.0
23	11980.0
24	11980.0
25	14424.5
26	16869.0
27	16869.0
28	21514.5
29	26160.0
30	32665.5
31	39171.0
32	39171.0
33	50101.5
34	61032.0
35	61032.0
36	67566.5
37	74101.0
38	83357.0
39	92613.0
40	92613.0
41	97529.5
42	102446.0
43	114091.5
44	125737.0
45	125737.0
46	129754.5
47	133772.0
48	133772.0
49	141512.5
50	149253.0
51	149810.0
52	150367.0
53	150367.0
54	142559.5
55	134752.0
56	134752.0
57	131020.5
58	127289.0
59	113803.5
60	100318.0
61	100318.0
62	94567.0
63	88816.0
64	73196.0
65	57576.0
66	57576.0
67	48242.0
68	38908.0
69	38908.0
70	31417.5
71	23927.0
72	18738.5
73	13550.0
74	13550.0
75	10091.0
76	6632.0
77	6632.0
78	6498.5
79	6365.0
80	4927.0
81	3489.0
82	3489.0
83	3378.5
84	3268.0
85	3268.0
86	2066.5
87	865.0
88	720.0
89	575.0
90	575.0
91	341.0
92	107.0
93	68.5
94	30.0
95	30.0
96	20.0
97	10.0
98	10.0
99	11.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00927452788015826
2	0.0
3	0.0
4	6.183018586772174E-5
5	6.183018586772174E-5
6	1.2366037173544347E-4
7	3.091509293386087E-4
8	0.0019167357618993738
9	0.004204452639005078
10	8.037924162803826E-4
11	0.005502886542227235
12	3.709811152063304E-4
13	6.183018586772174E-5
14	0.0
15	0.0
16	1.854905576031652E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.2366037173544347E-4
23	1.2366037173544347E-4
24	0.0
25	1.854905576031652E-4
26	6.183018586772174E-5
27	6.183018586772174E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.183018586772174E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1617333.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.0881356314915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.6509657468996	43.08172238497589
2	12.45793620930579	13.476530867548014
3	3.497002452318701	5.674390288340169
4	1.4697115307390967	3.17975826455133
5	0.7457327885168097	2.0167648105073783
6	0.49040094614363866	1.591492373329733
7	0.3210508669490381	1.2155529985303253
8	0.2105111032698855	0.9108922484477184
9	0.16088323164722443	0.7831686648750967
>10	0.8248884015091248	8.006598473057533
>50	0.07482999548360315	2.8372728503001463
>100	0.0808789511311337	9.529433650481531
>500	0.0110602007901402	4.210324520511652
>1k	0.004147575296302576	3.486097604543527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4278	0.26450953514211356	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3344	0.20676014154166147	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2061	0.1274320130733745	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1908	0.11797199463561307	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1890	0.11685905128999408	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1855	0.11469499478462383	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1817	0.11234544772165041	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1807	0.11172714586297318	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1756	0.10857380638371937	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1696	0.10486399523165607	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1660	0.10263810854041808	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1624	0.1004122218491801	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.183018586772174E-5
2	0.0	0.0	0.0	0.0	6.183018586772174E-5
3	0.0	0.0	0.0	0.0	6.183018586772174E-5
4	0.0	0.0	0.0	0.0	6.183018586772174E-5
5	6.183018586772174E-5	0.0	0.0	0.0	6.183018586772174E-5
6	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
7	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
8	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
9	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
10	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
11	6.183018586772174E-5	0.0	0.0	0.0	1.854905576031652E-4
12	6.183018586772174E-5	0.0	0.0	0.0	2.4732074347088694E-4
13	6.183018586772174E-5	0.0	0.0	0.0	3.091509293386087E-4
14	6.183018586772174E-5	0.0	0.0	0.0	3.709811152063304E-4
15	6.183018586772174E-5	0.0	0.0	0.0	4.3281130107405215E-4
16	6.183018586772174E-5	0.0	0.0	6.183018586772174E-5	5.564716728094956E-4
17	6.183018586772174E-5	0.0	0.0	6.183018586772174E-5	5.564716728094956E-4
18	6.183018586772174E-5	0.0	0.0	1.854905576031652E-4	5.564716728094956E-4
19	6.183018586772174E-5	0.0	0.0	1.854905576031652E-4	5.564716728094956E-4
20	6.183018586772174E-5	0.0	0.0	3.091509293386087E-4	5.564716728094956E-4
21	6.183018586772174E-5	0.0	0.0	3.091509293386087E-4	6.183018586772174E-4
22	6.183018586772174E-5	0.0	0.0	4.3281130107405215E-4	6.183018586772174E-4
23	6.183018586772174E-5	0.0	0.0	8.037924162803826E-4	8.037924162803826E-4
24	6.183018586772174E-5	0.0	0.0	9.892829738835478E-4	8.037924162803826E-4
25	6.183018586772174E-5	0.0	0.0	0.0014839244608253217	8.656226021481043E-4
26	6.183018586772174E-5	0.0	0.0	0.0019167357618993738	8.656226021481043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	385	0.0	20.36344	29
CATCGTT	410	0.0	19.121767	28
CGGCATC	435	0.0	18.758438	25
CGTTTAT	440	0.0	17.454376	31
AGGGGCG	360	0.0	16.888725	32
TCGTTTA	455	0.0	16.878958	30
GGCATCG	485	0.0	16.82458	26
TCTAGCG	290	0.0	16.551565	28
CTAGCGG	300	0.0	15.9998455	29
GCATCGT	525	0.0	15.8474655	27
GTAGGAC	705	0.0	15.432952	3
CGCATCG	545	0.0	15.265907	13
CGTACAT	95	1.4332403E-5	15.157748	26
TAACCCG	180	7.2759576E-12	15.110966	28
GTCCTAC	525	0.0	14.934575	1
TAAACGC	375	0.0	14.933189	28
GCATCGC	580	0.0	14.896409	14
CAATTGG	65	0.004160255	14.770457	1
TAGCGGC	315	0.0	14.730017	30
ACCGTCG	360	0.0	14.666979	8
>>END_MODULE