FastQCFastQC Report
Thu 2 Feb 2017
SRR4062821_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062821_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725981
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT63040.3652415640728374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT43140.24994481399273805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT23980.1389354807497881No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21960.12723199154567752No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19350.11211015648492076No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18990.11002438613171292No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18560.1075330493209369No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18070.1046940841179596No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18010.10434645572575828No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17880.10359326087598877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17770.10295594215695306No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA17510.10144955245741406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG552.2235563E-623.2679675
ATCGGTA701.7881332E-518.28568321
GTCCTAT4000.018.018241
TTAGGAC9250.017.6401443
GTAGGAC16650.017.3903393
TAGGACC11800.016.9451944
TAGGACG16800.016.8532074
TCTAGCG4200.016.75993228
TGTAGGA17200.016.7485242
AGGACGT17150.016.6962915
GTCCTAA7100.016.6929551
AAGACGG4800.016.6632585
GTCCTAC13350.016.1961731
CGATTAA908.585941E-615.99997124
CTAGCGG4400.015.99904429
AACGAAT2400.015.99904331
GGACGTG17300.015.904266
GACGTGA9000.015.8208187
CAAGACG5000.015.6763364
CTGTAGG18300.015.6661341
Produced by FastQC (version 0.11.5)