##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062821_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725981
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214789734070074	32.0	32.0	32.0	32.0	32.0
2	30.840592103852824	32.0	32.0	32.0	32.0	32.0
3	30.893575305869533	32.0	32.0	32.0	32.0	32.0
4	30.95395372254967	32.0	32.0	32.0	32.0	32.0
5	30.882680632057944	32.0	32.0	32.0	32.0	32.0
6	34.52733025450454	36.0	36.0	36.0	32.0	36.0
7	34.44585832636628	36.0	36.0	36.0	32.0	36.0
8	34.42371323902175	36.0	36.0	36.0	32.0	36.0
9	34.557206597291625	36.0	36.0	36.0	32.0	36.0
10	34.28921986974364	36.0	36.0	36.0	32.0	36.0
11	34.558065239420365	36.0	36.0	36.0	32.0	36.0
12	34.37567736840672	36.0	36.0	36.0	32.0	36.0
13	34.45964121273641	36.0	36.0	36.0	32.0	36.0
14	34.3724363130301	36.0	36.0	36.0	32.0	36.0
15	34.314444365262425	36.0	36.0	36.0	32.0	36.0
16	34.33360911852448	36.0	36.0	36.0	32.0	36.0
17	34.26270625227045	36.0	36.0	36.0	32.0	36.0
18	34.28886123311902	36.0	36.0	36.0	32.0	36.0
19	34.2653667682321	36.0	36.0	36.0	32.0	36.0
20	34.234981149850434	36.0	36.0	36.0	32.0	36.0
21	34.2082479471095	36.0	36.0	36.0	32.0	36.0
22	34.189163148377645	36.0	36.0	36.0	32.0	36.0
23	34.13919620204394	36.0	36.0	36.0	32.0	36.0
24	34.12821693865691	36.0	36.0	36.0	32.0	36.0
25	34.10288178143328	36.0	36.0	36.0	32.0	36.0
26	34.070914453867104	36.0	36.0	36.0	32.0	36.0
27	34.06298273271838	36.0	36.0	36.0	32.0	36.0
28	34.037929733873085	36.0	36.0	36.0	32.0	36.0
29	33.997395683961756	36.0	36.0	36.0	32.0	36.0
30	33.955344815499124	36.0	36.0	36.0	32.0	36.0
31	33.97648757431281	36.0	36.0	36.0	32.0	36.0
32	33.928860167058616	36.0	36.0	36.0	32.0	36.0
33	33.905840214927046	36.0	36.0	36.0	32.0	36.0
34	33.88534404492286	36.0	36.0	36.0	32.0	36.0
35	33.84205388124203	36.0	36.0	36.0	32.0	36.0
36	33.795570171398175	36.0	36.0	36.0	32.0	36.0
37	33.78481744584674	36.0	36.0	36.0	32.0	36.0
38	33.17649209348191	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	2.0
9	8.0
10	23.0
11	9.0
12	8.0
13	6.0
14	191.0
15	443.0
16	611.0
17	674.0
18	913.0
19	1212.0
20	1565.0
21	2292.0
22	3259.0
23	5260.0
24	7741.0
25	11486.0
26	16996.0
27	24627.0
28	34356.0
29	46486.0
30	62503.0
31	83636.0
32	113521.0
33	168221.0
34	373800.0
35	766127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19217940224775	17.515073747373624	11.696961118607216	25.595785731771407
2	15.90394733485912	19.720152671401706	39.32216123713922	25.05373875659995
3	18.823640529336846	24.167015300889684	28.976081567660344	28.03326260211313
4	12.134782374416472	15.601565075614612	36.590218682988755	35.67343386698016
5	13.991164414316545	37.179623111574614	33.9317197526036	14.897492721505238
6	33.51344461375342	36.131914227941394	17.1813530942021	13.173288064103083
7	29.831209034166655	30.882726982510235	20.94970917988089	18.336354803442216
8	27.45283100856038	34.3044945610452	19.523805385361896	18.71886904503252
9	27.618144885148908	14.159193591560475	18.975778314157687	39.24688320913293
10	15.83590676547101	26.998151763935642	31.995863195768177	25.17007827482517
11	37.158903066678484	20.933648292320875	23.08181227725893	18.825636363741708
12	25.132189602127546	23.79076787586982	29.405827582810428	21.671214939192204
13	29.93095655449159	19.854959509771255	25.388633762621502	24.825450173115645
14	23.06134084893547	20.822073063890343	25.527050141612857	30.58953594556133
15	24.58349849672339	28.855493044592954	22.719760032082263	23.84124842660139
16	24.613162219229707	26.05070843000415	25.63003103509817	23.706098315667973
17	23.158258297587768	26.595569597513247	26.29753213623841	23.948639968660576
18	23.940304664762728	24.801827687283772	28.430955033817195	22.8269126141363
19	25.295910444647884	25.085116985155224	26.07529000034765	23.543682569849235
20	25.50452581236912	24.6581160429648	26.258634735479564	23.578723409186512
21	26.327481442810946	24.41761627829497	24.870776142735217	24.384126136158866
22	25.27184981872816	25.5420338022587	25.50292288892327	23.683193490089874
23	23.35056970416176	25.396336093006873	26.508800515468696	24.744293687362674
24	24.448248307528807	25.54500093004066	26.396234407332315	23.610516355098216
25	24.39687403660942	25.226751719326224	26.437002079185202	23.939372164879156
26	23.160712888729208	26.485479426652486	27.147720720538	23.206086964080306
27	24.185594285412982	25.858877096166538	26.15241718617545	23.803111432245025
28	23.207371050364248	25.930550493746114	26.36087994173431	24.50119851415533
29	23.510627572004537	25.688175993195017	26.342831183791343	24.458365251009102
30	23.272942598383363	25.930470987475136	26.92216994113888	23.874416473002626
31	24.576116512842383	25.29528294726132	25.691311192447706	24.437289347448587
32	23.565437821394944	26.105830375896282	25.681581806330122	24.647149996378648
33	23.073722855278756	25.745056615843165	26.821111086646404	24.360109442231675
34	24.040371497509522	25.846751482814646	26.807017381206787	23.305859638469045
35	24.474735498944778	25.920408042640037	26.631480985709004	22.97337547270618
36	23.28118363342734	26.40118395792638	26.28969535914221	24.027937049504068
37	24.19845209544233	26.338192288058355	25.440994033550485	24.02236158294883
38	23.56525699293811	26.744613195324828	25.79762023403659	23.892509577700473
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	142.0
1	196.0
2	250.0
3	250.0
4	717.0
5	1184.0
6	1184.0
7	1555.5
8	1927.0
9	2014.5
10	2102.0
11	2102.0
12	2647.5
13	3193.0
14	4065.5
15	4938.0
16	4938.0
17	7405.0
18	9872.0
19	9872.0
20	11824.5
21	13777.0
22	14671.0
23	15565.0
24	15565.0
25	19149.0
26	22733.0
27	22733.0
28	29633.0
29	36533.0
30	46377.0
31	56221.0
32	56221.0
33	68684.0
34	81147.0
35	81147.0
36	87512.5
37	93878.0
38	103635.0
39	113392.0
40	113392.0
41	115125.0
42	116858.0
43	125419.5
44	133981.0
45	133981.0
46	136461.5
47	138942.0
48	138942.0
49	144158.5
50	149375.0
51	147051.5
52	144728.0
53	144728.0
54	137306.5
55	129885.0
56	129885.0
57	125146.5
58	120408.0
59	107557.0
60	94706.0
61	94706.0
62	89757.5
63	84809.0
64	70028.5
65	55248.0
66	55248.0
67	46701.0
68	38154.0
69	38154.0
70	30774.0
71	23394.0
72	18487.0
73	13580.0
74	13580.0
75	10378.5
76	7177.0
77	7177.0
78	6879.0
79	6581.0
80	5140.5
81	3700.0
82	3700.0
83	3681.5
84	3663.0
85	3663.0
86	2504.0
87	1345.0
88	1121.5
89	898.0
90	898.0
91	660.5
92	423.0
93	366.5
94	310.0
95	310.0
96	277.0
97	244.0
98	244.0
99	481.0
100	718.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12300251277389497
2	0.056141985340510694
3	0.011529675008009938
4	0.003360407791279278
5	3.4762839220130464E-4
6	6.373187190357252E-4
7	0.0
8	3.4762839220130464E-4
9	4.0556645756818874E-4
10	6.373187190357252E-4
11	0.0024333987454091327
12	0.0029548413337110897
13	0.014832144733922331
14	0.008632771739665732
15	0.024160173257990674
16	0.011182046615808633
17	0.020799765466711393
18	0.004866797490818265
19	0.007010505909392978
20	0.005967620732789063
21	0.005793806536688411
22	0.006952567844026093
23	0.01002328530847095
24	0.014484516341721026
25	0.017671109936899652
26	0.01888780930960422
27	0.008806585935766385
28	0.005446178144487107
29	0.010834418223607329
30	0.0031286555298117415
31	0.005446178144487107
32	0.0061414349288897154
33	0.00805339108599689
34	0.012109055661678778
35	0.016164720237360666
36	0.014136887949519722
37	0.009501842720168994
38	0.005619992340587758
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1725981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.23701259156167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.60232983477768	39.24874538287464
2	13.733570259107609	14.073342245859868
3	4.512221239543661	6.935782093992317
4	1.8705366917852697	3.833628481199199
5	0.9716915295936219	2.4893285568451113
6	0.5320737553355467	1.6357121821060143
7	0.3612019337126101	1.2954835618010598
8	0.2358336131112244	0.966672784359463
9	0.1673585812854684	0.7717458362966498
>10	0.8204542191041164	7.636394984742396
>50	0.08651527764406931	3.0924433866349483
>100	0.08993422933246861	9.973317220671547
>500	0.01138380116556683	3.9458931613561448
>1k	0.004781196489538069	3.7338154753693753
>5k	1.138380116556683E-4	0.36769464589125267
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6304	0.3652415640728374	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4314	0.24994481399273805	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2398	0.1389354807497881	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2196	0.12723199154567752	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1935	0.11211015648492076	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1899	0.11002438613171292	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1856	0.1075330493209369	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1807	0.1046940841179596	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1801	0.10434645572575828	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1788	0.10359326087598877	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1777	0.10295594215695306	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1751	0.10144955245741406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1587613073376821E-4
2	0.0	0.0	0.0	0.0	1.1587613073376821E-4
3	0.0	0.0	0.0	0.0	1.1587613073376821E-4
4	0.0	0.0	0.0	0.0	1.1587613073376821E-4
5	0.0	5.7938065366884106E-5	0.0	0.0	1.1587613073376821E-4
6	0.0	5.7938065366884106E-5	0.0	0.0	2.3175226146753642E-4
7	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	2.3175226146753642E-4
8	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	2.3175226146753642E-4
9	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	2.3175226146753642E-4
10	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	2.3175226146753642E-4
11	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	2.3175226146753642E-4
12	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	4.0556645756818874E-4
13	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	5.793806536688411E-4
14	0.0	1.1587613073376821E-4	0.0	5.7938065366884106E-5	5.793806536688411E-4
15	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	7.531948497694934E-4
16	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	7.531948497694934E-4
17	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	7.531948497694934E-4
18	0.0	1.7381419610065232E-4	0.0	1.7381419610065232E-4	7.531948497694934E-4
19	0.0	1.7381419610065232E-4	0.0	2.3175226146753642E-4	7.531948497694934E-4
20	0.0	1.7381419610065232E-4	0.0	2.3175226146753642E-4	9.270090458701457E-4
21	0.0	1.7381419610065232E-4	0.0	2.3175226146753642E-4	0.001042885176603914
22	0.0	1.7381419610065232E-4	0.0	6.952567844026093E-4	0.001042885176603914
23	0.0	1.7381419610065232E-4	0.0	0.0012166993727045663	0.001042885176603914
24	0.0	1.7381419610065232E-4	0.0	0.0018540180917402914	0.001042885176603914
25	0.0	1.7381419610065232E-4	0.0	0.0023754606800422485	0.001042885176603914
26	0.0	1.7381419610065232E-4	0.0	0.003302469725912394	0.0011008232419707981
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	55	2.2235563E-6	23.267967	5
ATCGGTA	70	1.7881332E-5	18.285683	21
GTCCTAT	400	0.0	18.01824	1
TTAGGAC	925	0.0	17.640144	3
GTAGGAC	1665	0.0	17.390339	3
TAGGACC	1180	0.0	16.945194	4
TAGGACG	1680	0.0	16.853207	4
TCTAGCG	420	0.0	16.759932	28
TGTAGGA	1720	0.0	16.748524	2
AGGACGT	1715	0.0	16.696291	5
GTCCTAA	710	0.0	16.692955	1
AAGACGG	480	0.0	16.663258	5
GTCCTAC	1335	0.0	16.196173	1
CGATTAA	90	8.585941E-6	15.999971	24
CTAGCGG	440	0.0	15.999044	29
AACGAAT	240	0.0	15.999043	31
GGACGTG	1730	0.0	15.90426	6
GACGTGA	900	0.0	15.820818	7
CAAGACG	500	0.0	15.676336	4
CTGTAGG	1830	0.0	15.666134	1
>>END_MODULE