FastQCFastQC Report
Thu 2 Feb 2017
SRR4062821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1725981
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT71060.41170789249707845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT53120.3077670032288884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT31370.18175171105591545No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22870.13250435549406395No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20850.12080086628995336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.12051117596311893No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20310.11767221076014163No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19590.11350067005372598No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19140.1108934571122162No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18870.10932912934731032No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17660.10231862343791734No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17590.10191305698034915No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA17300.1002328530847095No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17300.1002328530847095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4350.018.39007228
CTAGCGG4350.018.39007229
TATGACG450.00885109817.77814
CGAAATT450.00885405317.7770719
TAGCGGC4600.017.39061230
ATACCGT4150.016.5805366
TAGGACG16200.016.4941274
GTCCTAC13500.016.3558521
TGTAGGA16150.016.2475012
ACCGTCG4000.016.0021448
TACCGTC3900.016.0021427
CGTCGTA4000.016.00121710
TAAACGC4000.015.99936228
AGGACGT16650.015.9540895
TTAGGAC8150.015.9021293
GGACGTG16450.015.8562276
CGCTTCG3750.015.78603732
GTAGGAC16650.015.7600443
TCCTACA15050.015.7340492
GTCCTAG2700.015.4076861
Produced by FastQC (version 0.11.5)