##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1725981
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176668225200626	32.0	32.0	32.0	32.0	32.0
2	31.305244959243467	32.0	32.0	32.0	32.0	32.0
3	31.379526194089042	32.0	32.0	32.0	32.0	32.0
4	31.483745186070994	32.0	32.0	32.0	32.0	32.0
5	31.40332077815457	32.0	32.0	32.0	32.0	32.0
6	34.92595689060308	36.0	36.0	36.0	36.0	36.0
7	34.95342880367745	36.0	36.0	36.0	36.0	36.0
8	34.895235810822946	36.0	36.0	36.0	36.0	36.0
9	34.98839384674571	36.0	36.0	36.0	36.0	36.0
10	34.853745782833066	36.0	36.0	36.0	32.0	36.0
11	35.02148169649608	36.0	36.0	36.0	36.0	36.0
12	34.92342094148197	36.0	36.0	36.0	32.0	36.0
13	34.97892792562607	36.0	36.0	36.0	36.0	36.0
14	34.92086529341864	36.0	36.0	36.0	32.0	36.0
15	34.89195477818122	36.0	36.0	36.0	32.0	36.0
16	34.90082741351151	36.0	36.0	36.0	32.0	36.0
17	34.873837545140994	36.0	36.0	36.0	32.0	36.0
18	34.86932011418434	36.0	36.0	36.0	32.0	36.0
19	34.851918416251394	36.0	36.0	36.0	32.0	36.0
20	34.83849416650589	36.0	36.0	36.0	32.0	36.0
21	34.828443650306696	36.0	36.0	36.0	32.0	36.0
22	34.8065256801784	36.0	36.0	36.0	32.0	36.0
23	34.75748110784534	36.0	36.0	36.0	32.0	36.0
24	34.73294781344638	36.0	36.0	36.0	32.0	36.0
25	34.71265558543229	36.0	36.0	36.0	32.0	36.0
26	34.654522268785115	36.0	36.0	36.0	32.0	36.0
27	34.62665811500822	36.0	36.0	36.0	32.0	36.0
28	34.587720838178406	36.0	36.0	36.0	32.0	36.0
29	34.54957963036673	36.0	36.0	36.0	32.0	36.0
30	34.51825483594547	36.0	36.0	36.0	32.0	36.0
31	34.52161234683348	36.0	36.0	36.0	32.0	36.0
32	34.48568900816405	36.0	36.0	36.0	32.0	36.0
33	34.44968803248703	36.0	36.0	36.0	32.0	36.0
34	34.43874527008119	36.0	36.0	36.0	32.0	36.0
35	34.391427831476705	36.0	36.0	36.0	32.0	36.0
36	34.355237977706594	36.0	36.0	36.0	32.0	36.0
37	34.3320720216503	36.0	36.0	36.0	32.0	36.0
38	33.90690859285241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	28.0
22	127.0
23	382.0
24	1098.0
25	2762.0
26	5935.0
27	11634.0
28	20188.0
29	32662.0
30	49562.0
31	70395.0
32	100627.0
33	159550.0
34	385228.0
35	885792.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.512841758821196	18.790716270283895	12.367316311423878	26.329125659471032
2	15.00804470037619	20.800286909299697	38.39781550318341	25.793852887140705
3	17.866535031382153	24.849636235856597	29.302060683170904	27.98176804959035
4	11.644419363641578	16.46470375908833	36.649097873262505	35.24177900400758
5	13.4084983603518	37.75113268983418	33.635094265286966	15.205274684527051
6	33.50635580945318	36.52805942131427	16.81902455416633	13.146560215066222
7	29.507604990115716	31.127214114713333	20.99205549105084	18.373125404120113
8	27.408641723524173	34.514285449421536	19.321336862207062	18.755735964847226
9	27.43429129973527	13.973241988443219	18.78072649084861	39.8117402209729
10	15.650619651558253	27.24282577333326	31.979466618770896	25.127087956337597
11	37.16270437923005	20.84556610272803	23.07675371125855	18.91497580678337
12	24.8575154825354	23.989890942926817	29.39494558733981	21.757647987197977
13	29.889662556533164	19.866591578803437	25.44030109306144	24.803444771601956
14	22.828235073271376	20.7661613887986	25.596342022304995	30.809261515625025
15	24.58213618805769	28.959241150395048	22.563110486152514	23.895512175394746
16	24.52842760146259	26.092813304433825	25.524267068988593	23.854492025114993
17	23.076325869172372	26.73662108679064	26.207936240317824	23.97911680371916
18	23.954145497546033	24.831791311723595	28.38020812511841	22.833855065611964
19	25.34245741986731	25.05960378474618	26.123288726816806	23.474650068569698
20	25.481219086420996	24.68769934315615	26.334183284752267	23.496898285670582
21	26.170971754613753	24.440535556301025	25.025304450048985	24.363188239036234
22	25.166905081805652	25.51204213719618	25.680641907413815	23.640410873584354
23	23.196386980150407	25.251741039873	26.645673762152516	24.906198217824077
24	24.41944610050748	25.453988195698564	26.346350278479314	23.780215425314648
25	24.3155615631031	25.145352458346782	26.508290666677482	24.030795311872637
26	23.226906901060904	26.343685127472433	27.117563866577903	23.311844104888756
27	24.240895027752348	25.693576982352056	26.181241961088773	23.884286028806823
28	23.16601399436031	25.79078216967626	26.56993327273012	24.473270563233314
29	23.53432627589759	25.427684314022	26.458576311094966	24.579413098985448
30	23.345679935063014	25.62606424983821	27.069996714911692	23.95825910018708
31	24.48080251173101	25.099928678241533	25.84153591493765	24.577732895089806
32	23.538613692734742	25.926936623288437	25.875777311569482	24.658672372407345
33	23.147126185050702	25.52843860969501	26.939983696228403	24.38445150902588
34	23.993717196191618	25.661464407777373	26.952961822870584	23.39185657316042
35	24.438507724013185	25.741360999918307	26.683260128587744	23.13687114748077
36	23.29226103879475	26.15422765372272	26.509503870552457	24.04400743693007
37	24.36417318614747	25.988872415165638	25.60653912180957	24.040415276877326
38	23.710253716876046	26.337863902167985	25.955240475115858	23.996641905840104
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	95.0
2	127.0
3	127.0
4	576.0
5	1025.0
6	1025.0
7	1449.5
8	1874.0
9	2053.5
10	2233.0
11	2233.0
12	2879.0
13	3525.0
14	4294.5
15	5064.0
16	5064.0
17	7901.5
18	10739.0
19	10739.0
20	12455.0
21	14171.0
22	14990.0
23	15809.0
24	15809.0
25	19050.0
26	22291.0
27	22291.0
28	29265.5
29	36240.0
30	45830.0
31	55420.0
32	55420.0
33	68685.5
34	81951.0
35	81951.0
36	88177.0
37	94403.0
38	103850.5
39	113298.0
40	113298.0
41	114631.0
42	115964.0
43	124748.0
44	133532.0
45	133532.0
46	136727.5
47	139923.0
48	139923.0
49	145049.0
50	150175.0
51	147827.5
52	145480.0
53	145480.0
54	137699.5
55	129919.0
56	129919.0
57	125338.5
58	120758.0
59	107836.5
60	94915.0
61	94915.0
62	89810.5
63	84706.0
64	70193.5
65	55681.0
66	55681.0
67	46853.0
68	38025.0
69	38025.0
70	30793.0
71	23561.0
72	18425.5
73	13290.0
74	13290.0
75	10080.0
76	6870.0
77	6870.0
78	6719.0
79	6568.0
80	5017.0
81	3466.0
82	3466.0
83	3349.0
84	3232.0
85	3232.0
86	2067.5
87	903.0
88	749.5
89	596.0
90	596.0
91	346.0
92	96.0
93	69.5
94	43.0
95	43.0
96	37.0
97	31.0
98	31.0
99	22.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009617718850902762
2	0.0
3	0.0
4	2.3175226146753642E-4
5	5.7938065366884106E-5
6	5.7938065366884106E-5
7	5.21442588301957E-4
8	0.001680203895639639
9	0.003939788444948119
10	6.373187190357252E-4
11	0.004866797490818265
12	2.3175226146753642E-4
13	1.7381419610065232E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	5.7938065366884106E-5
24	0.0
25	2.3175226146753642E-4
26	0.0
27	5.7938065366884106E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.1587613073376821E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1725981.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.97211472801696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73724336810146	36.6004085667839
2	14.650840467188692	14.349652804420765
3	4.901847850003993	7.201615658690365
4	2.109719219507098	4.13269646646416
5	1.0700813078205091	2.6202072287446208
6	0.6128461203899567	1.800742231101423
7	0.36560171070828107	1.2533002245095646
8	0.25299336252804777	0.9911695980120274
9	0.18422998917317362	0.8119918949516984
>10	0.9087254821207038	8.06183565795237
>50	0.08926487015548139	3.048878933571525
>100	0.09766852333914398	10.136828449688547
>500	0.013697099564762463	4.59405686114323
>1k	0.005002418971478465	3.672294074816746
>5k	2.3821042721326025E-4	0.7243213491490392
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7106	0.41170789249707845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5312	0.3077670032288884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3137	0.18175171105591545	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2287	0.13250435549406395	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2085	0.12080086628995336	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2080	0.12051117596311893	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2031	0.11767221076014163	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1959	0.11350067005372598	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1914	0.1108934571122162	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1887	0.10932912934731032	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1766	0.10231862343791734	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1759	0.10191305698034915	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1730	0.1002328530847095	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1730	0.1002328530847095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7381419610065232E-4
2	0.0	0.0	0.0	0.0	1.7381419610065232E-4
3	0.0	0.0	0.0	0.0	1.7381419610065232E-4
4	0.0	0.0	0.0	0.0	1.7381419610065232E-4
5	0.0	0.0	0.0	0.0	1.7381419610065232E-4
6	0.0	0.0	0.0	0.0	2.8969032683442053E-4
7	0.0	0.0	0.0	0.0	2.8969032683442053E-4
8	0.0	0.0	0.0	0.0	2.8969032683442053E-4
9	0.0	0.0	0.0	0.0	2.8969032683442053E-4
10	0.0	0.0	0.0	0.0	2.8969032683442053E-4
11	0.0	0.0	0.0	0.0	2.8969032683442053E-4
12	0.0	0.0	0.0	0.0	4.0556645756818874E-4
13	0.0	0.0	0.0	0.0	5.21442588301957E-4
14	0.0	0.0	0.0	0.0	5.21442588301957E-4
15	0.0	0.0	0.0	5.7938065366884106E-5	6.373187190357252E-4
16	0.0	0.0	0.0	5.7938065366884106E-5	6.952567844026093E-4
17	0.0	0.0	0.0	1.1587613073376821E-4	7.531948497694934E-4
18	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	7.531948497694934E-4
19	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	7.531948497694934E-4
20	0.0	1.1587613073376821E-4	0.0	1.1587613073376821E-4	9.270090458701457E-4
21	0.0	1.1587613073376821E-4	0.0	1.7381419610065232E-4	9.8494711123703E-4
22	0.0	1.1587613073376821E-4	0.0	6.952567844026093E-4	9.8494711123703E-4
23	0.0	1.1587613073376821E-4	0.0	0.0012166993727045663	9.8494711123703E-4
24	0.0	1.1587613073376821E-4	0.0	0.0017381419610065232	9.8494711123703E-4
25	0.0	1.1587613073376821E-4	0.0	0.0023175226146753642	9.8494711123703E-4
26	0.0	1.1587613073376821E-4	0.0	0.003186593595178626	0.001042885176603914
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	435	0.0	18.390072	28
CTAGCGG	435	0.0	18.390072	29
TATGACG	45	0.008851098	17.7781	4
CGAAATT	45	0.008854053	17.77707	19
TAGCGGC	460	0.0	17.390612	30
ATACCGT	415	0.0	16.580536	6
TAGGACG	1620	0.0	16.494127	4
GTCCTAC	1350	0.0	16.355852	1
TGTAGGA	1615	0.0	16.247501	2
ACCGTCG	400	0.0	16.002144	8
TACCGTC	390	0.0	16.002142	7
CGTCGTA	400	0.0	16.001217	10
TAAACGC	400	0.0	15.999362	28
AGGACGT	1665	0.0	15.954089	5
TTAGGAC	815	0.0	15.902129	3
GGACGTG	1645	0.0	15.856227	6
CGCTTCG	375	0.0	15.786037	32
GTAGGAC	1665	0.0	15.760044	3
TCCTACA	1505	0.0	15.734049	2
GTCCTAG	270	0.0	15.407686	1
>>END_MODULE