FastQCFastQC Report
Thu 2 Feb 2017
SRR4062820_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062820_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718865
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48410.2816393375861397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33320.1938488479316293No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26110.15190256361028934No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25280.14707379578966354No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23620.1374162601484119No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23430.13631087956296742No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23360.13590363408411946No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC23260.13532185482862238No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22950.1335183391365814No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21750.12653698807061636No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21510.12514071785742334No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20890.12153368647334142No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19890.11571589391837056No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18510.10768734019251075No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18450.1073382726392125No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18380.10693102716036454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17730.10314946199963348No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17620.1025095048185867No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17200.10006603194549892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT4250.019.19916328
GCATCGT4500.019.19860327
CGGCATC4300.018.9770225
ACGATTA852.398083E-718.82325732
TATAACG601.1377099E-418.6685682
CGTTTAT4350.018.39053731
TCTAGCG5600.017.71351228
ATCGTTT4650.017.5481329
TAGCGGC5700.017.40325530
TACCGTC4700.017.3599367
CTAGCGG5850.017.23051829
GTCCTAC5600.016.8711341
ATACCGT4900.016.322026
GGCATCG5200.016.30745526
ACCGTCG4900.015.9983718
TACGACT600.002446601215.9960444
TAGCCCG600.002447076415.9955795
TCGTTTA5050.015.84135330
CGTCGTA4750.015.83227110
TAGGACG4750.015.8276644
Produced by FastQC (version 0.11.5)