##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062820_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1718865 Sequences flagged as poor quality 0 Sequence length 38 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.209165932170357 32.0 32.0 32.0 32.0 32.0 2 30.75899328917629 32.0 32.0 32.0 32.0 32.0 3 30.81922140482237 32.0 32.0 32.0 32.0 32.0 4 30.896232106651773 32.0 32.0 32.0 32.0 32.0 5 30.782399432183446 32.0 32.0 32.0 32.0 32.0 6 34.4382432593601 36.0 36.0 36.0 32.0 36.0 7 34.36882303147717 36.0 36.0 36.0 32.0 36.0 8 34.33189692035151 36.0 36.0 36.0 32.0 36.0 9 34.50079732846966 36.0 36.0 36.0 32.0 36.0 10 34.172691281746964 36.0 36.0 36.0 32.0 36.0 11 34.499943276522586 36.0 36.0 36.0 32.0 36.0 12 34.283889077967146 36.0 36.0 36.0 32.0 36.0 13 34.39324845173996 36.0 36.0 36.0 32.0 36.0 14 34.27787464402382 36.0 36.0 36.0 32.0 36.0 15 34.20297696445038 36.0 36.0 36.0 32.0 36.0 16 34.231389899730345 36.0 36.0 36.0 32.0 36.0 17 34.140100007854016 36.0 36.0 36.0 32.0 36.0 18 34.18142902438528 36.0 36.0 36.0 32.0 36.0 19 34.15763599817321 36.0 36.0 36.0 32.0 36.0 20 34.14283087967932 36.0 36.0 36.0 32.0 36.0 21 34.12042539699162 36.0 36.0 36.0 32.0 36.0 22 34.090754654961266 36.0 36.0 36.0 32.0 36.0 23 34.04446538849764 36.0 36.0 36.0 32.0 36.0 24 34.0182667050641 36.0 36.0 36.0 32.0 36.0 25 33.9954702667167 36.0 36.0 36.0 32.0 36.0 26 33.93875609777382 36.0 36.0 36.0 32.0 36.0 27 33.949568465237235 36.0 36.0 36.0 32.0 36.0 28 33.92624028065031 36.0 36.0 36.0 32.0 36.0 29 33.88033557027457 36.0 36.0 36.0 32.0 36.0 30 33.85554595619784 36.0 36.0 36.0 32.0 36.0 31 33.88456336012427 36.0 36.0 36.0 32.0 36.0 32 33.82132919106503 36.0 36.0 36.0 32.0 36.0 33 33.781399353643245 36.0 36.0 36.0 32.0 36.0 34 33.788327181017706 36.0 36.0 36.0 32.0 36.0 35 33.73993885500025 36.0 36.0 36.0 32.0 36.0 36 33.69495161051042 36.0 36.0 36.0 27.0 36.0 37 33.69301021313483 36.0 36.0 36.0 27.0 36.0 38 33.06817696561394 36.0 36.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 3.0 9 9.0 10 12.0 11 16.0 12 9.0 13 13.0 14 195.0 15 392.0 16 554.0 17 620.0 18 822.0 19 1079.0 20 1497.0 21 2221.0 22 3570.0 23 5404.0 24 8517.0 25 12667.0 26 18557.0 27 26325.0 28 36341.0 29 48888.0 30 65817.0 31 88150.0 32 119809.0 33 177331.0 34 380836.0 35 719209.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.70159492707998 17.122910673482902 11.336196276853201 25.83929812258392 2 16.778125409275454 19.562857433897467 37.50508592965555 26.153931227171523 3 18.888790622603548 23.773510693634556 28.489904496807444 28.847794186954452 4 12.305410768943451 15.037005189046162 36.21918149327472 36.43840254873567 5 14.657979508499533 36.60131517477582 33.42897817680217 15.311727139922471 6 34.51857833432333 35.634494318026455 16.650657966311332 13.19626938133888 7 30.517230847099686 30.39430089041315 20.68894299435965 18.399525268127515 8 28.43423639258788 32.80893568473541 19.344030727324665 19.412797195352038 9 27.72807108893622 13.861912045243916 18.584457963732962 39.825558902086904 10 16.00067253916697 26.156304851066896 31.27679466436747 26.566227945398662 11 38.0627936580337 20.834041176703305 22.161742016108576 18.941423149154428 12 25.01458852222197 23.563765077957687 28.577736896947144 22.843909502873196 13 29.647003785635068 19.041115976823022 25.396805077615408 25.9150751599265 14 23.728939787735285 19.873648908508336 24.80754382810759 31.589867475648788 15 25.261197113185606 27.42793729639893 22.192298336741477 25.118567253673984 16 25.999029480970147 25.50434406179669 23.88524136133331 24.61138509589985 17 23.991876589331333 25.76232622825853 25.3947360764392 24.85106110597094 18 25.11419536702225 24.280399007426844 26.718952270962 23.886453354588905 19 25.875776954043417 24.641261488854276 25.329909101540615 24.153052455561692 20 25.803178444988117 24.035746597120607 25.01994164414565 25.141133313745623 21 27.219977367206887 23.970571550753604 24.09659373554541 24.7128573464941 22 25.900519569682267 24.279438888016152 24.926253687315633 24.89378785498595 23 24.237720174574516 24.057816026480587 25.654203513949263 26.050260284995634 24 24.919688330791562 25.042403281242997 25.198924484047346 24.83898390391809 25 24.9565478985592 24.37943649301537 25.36665892762483 25.297356680800604 26 24.431381425853953 25.533442691824938 25.947232103215175 24.087943779105935 27 25.35970605604834 24.790786032425764 24.83448197615051 25.015025935375384 28 24.241837138402104 24.700365379692336 25.837289673950988 25.220507807954572 29 24.324761499128385 24.880895027032814 25.682569827981467 25.111773645857333 30 24.352313519237146 25.119821271693787 26.022713388914426 24.50515182015464 31 25.15442828226645 24.7462267833943 24.688627085007266 25.410717849331988 32 24.54404284051114 25.011709111346814 24.66296668445094 25.781281363691104 33 24.252636282602978 24.43649532090384 25.55815182101904 25.752716575474132 34 25.147166210393713 24.75237615898664 25.650065313460974 24.450392317158677 35 25.816028228152742 24.64726446669553 25.488429902104542 24.048277403047187 36 24.309131227390715 25.408807300373503 25.11234454616178 25.169716926074 37 25.59575863258724 25.100730730538533 24.770304842464412 24.533205794409817 38 24.666270255929287 24.956363788693682 25.346064246466632 25.031301708910398 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 123.0 1 172.0 2 221.0 3 221.0 4 590.0 5 959.0 6 959.0 7 1219.5 8 1480.0 9 1520.5 10 1561.0 11 1561.0 12 2072.5 13 2584.0 14 3334.0 15 4084.0 16 4084.0 17 6009.5 18 7935.0 19 7935.0 20 9468.5 21 11002.0 22 11996.5 23 12991.0 24 12991.0 25 15493.0 26 17995.0 27 17995.0 28 23453.5 29 28912.0 30 35173.5 31 41435.0 32 41435.0 33 52772.0 34 64109.0 35 64109.0 36 69997.0 37 75885.0 38 85850.5 39 95816.0 40 95816.0 41 100194.5 42 104573.0 43 118022.0 44 131471.0 45 131471.0 46 134800.5 47 138130.0 48 138130.0 49 145502.5 50 152875.0 51 154095.5 52 155316.0 53 155316.0 54 148181.0 55 141046.0 56 141046.0 57 137593.0 58 134140.0 59 120698.5 60 107257.0 61 107257.0 62 103320.0 63 99383.0 64 82888.0 65 66393.0 66 66393.0 67 56033.0 68 45673.0 69 45673.0 70 36991.5 71 28310.0 72 22462.5 73 16615.0 74 16615.0 75 12653.5 76 8692.0 77 8692.0 78 8330.5 79 7969.0 80 6279.5 81 4590.0 82 4590.0 83 4512.0 84 4434.0 85 4434.0 86 3036.5 87 1639.0 88 1378.5 89 1118.0 90 1118.0 91 820.0 92 522.0 93 459.5 94 397.0 95 397.0 96 354.5 97 312.0 98 312.0 99 615.0 100 918.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11542500429062201 2 0.05177835373924072 3 0.010413848673397853 4 0.003025252128584851 5 3.4906755329825205E-4 6 4.6542340439766934E-4 7 0.0 8 2.3271170219883467E-4 9 2.3271170219883467E-4 10 3.4906755329825205E-4 11 0.0016871598409415518 12 0.0027925404263860164 13 0.01530079441957338 14 0.009424823939052805 15 0.025074685911924438 16 0.011461051333292609 17 0.021816722081140754 18 0.0058177925549708675 19 0.007795842023660962 20 0.0058177925549708675 21 0.006981351065965041 22 0.007854019949210672 23 0.010006603194549891 24 0.014428125536327751 25 0.017802445218210852 26 0.020827697346795706 27 0.009890247343450474 28 0.0061086821827194115 29 0.012391898142087947 30 0.0031997859052339773 31 0.00616686010826912 32 0.006864995214865624 33 0.009133934311304262 34 0.013380922876432994 35 0.01727884388826348 36 0.015591684047321924 37 0.01012295904564931 38 0.006341393884918245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 1718865.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.36549247860173 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58928762517837 37.04278614577029 2 13.590360500277962 13.146089571153595 3 4.342057918053958 6.300173087318753 4 1.8820669615340186 3.641083818891935 5 0.988150697403967 2.389619756150831 6 0.5615718670807951 1.6296419948094314 7 0.37793067446094475 1.2795162235151591 8 0.26595642229128075 1.0290490673571813 9 0.1901537899555472 0.8277193528085366 >10 1.0015933558136076 8.768477272973584 >50 0.09191898400779999 3.1393342483241127 >100 0.09687431463832129 10.276415756979326 >500 0.015441758638452239 5.154361605750514 >1k 0.006635130664959946 5.375732098196857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 4841 0.2816393375861397 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 3332 0.1938488479316293 No Hit GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC 2611 0.15190256361028934 No Hit GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT 2528 0.14707379578966354 No Hit GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG 2362 0.1374162601484119 No Hit GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG 2343 0.13631087956296742 No Hit GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA 2336 0.13590363408411946 No Hit GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC 2326 0.13532185482862238 No Hit GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG 2295 0.1335183391365814 No Hit GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC 2175 0.12653698807061636 No Hit GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG 2151 0.12514071785742334 No Hit CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA 2089 0.12153368647334142 No Hit GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG 1989 0.11571589391837056 No Hit GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT 1851 0.10768734019251075 No Hit CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT 1845 0.1073382726392125 No Hit GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA 1838 0.10693102716036454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 1773 0.10314946199963348 No Hit ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG 1762 0.1025095048185867 No Hit CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT 1720 0.10006603194549892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 1.1635585109941735E-4 11 0.0 0.0 0.0 0.0 1.1635585109941735E-4 12 0.0 0.0 0.0 0.0 5.236013299473781E-4 13 0.0 0.0 0.0 0.0 7.563130321462128E-4 14 0.0 0.0 0.0 0.0 7.563130321462128E-4 15 0.0 0.0 0.0 5.8177925549708675E-5 9.890247343450475E-4 16 0.0 0.0 0.0 1.1635585109941735E-4 0.0010472026598947561 17 0.0 0.0 0.0 2.327117021988347E-4 0.0010472026598947561 18 0.0 0.0 0.0 3.4906755329825205E-4 0.0010472026598947561 19 0.0 0.0 0.0 3.4906755329825205E-4 0.0010472026598947561 20 0.0 0.0 0.0 4.072454788479607E-4 0.0011635585109941735 21 0.0 0.0 0.0 4.654234043976694E-4 0.0011635585109941735 22 0.0 0.0 0.0 9.308468087953388E-4 0.0011635585109941735 23 0.0 0.0 0.0 0.0015708039898421342 0.0011635585109941735 24 0.0 0.0 0.0 0.0023852949475380554 0.0011635585109941735 25 0.0 0.0 0.0 0.002676184575286599 0.0011635585109941735 26 0.0 0.0 0.0 0.003374319681883103 0.0012799143620935908 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCGTT 425 0.0 19.199163 28 GCATCGT 450 0.0 19.198603 27 CGGCATC 430 0.0 18.97702 25 ACGATTA 85 2.398083E-7 18.823257 32 TATAACG 60 1.1377099E-4 18.668568 2 CGTTTAT 435 0.0 18.390537 31 TCTAGCG 560 0.0 17.713512 28 ATCGTTT 465 0.0 17.54813 29 TAGCGGC 570 0.0 17.403255 30 TACCGTC 470 0.0 17.359936 7 CTAGCGG 585 0.0 17.230518 29 GTCCTAC 560 0.0 16.871134 1 ATACCGT 490 0.0 16.32202 6 GGCATCG 520 0.0 16.307455 26 ACCGTCG 490 0.0 15.998371 8 TACGACT 60 0.0024466012 15.996044 4 TAGCCCG 60 0.0024470764 15.995579 5 TCGTTTA 505 0.0 15.841353 30 CGTCGTA 475 0.0 15.832271 10 TAGGACG 475 0.0 15.827664 4 >>END_MODULE