FastQCFastQC Report
Thu 2 Feb 2017
SRR4062820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718865
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT53160.30927385222225134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT38350.22311234448313277No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28190.16400357212462874No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC25090.14596841520421905No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24880.1447466787676752No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24830.14445578913992665No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24170.14061604605364586No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23980.1395106654682014No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG23260.13532185482862238No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22340.12996948567804917No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21970.12781690243270996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21660.126013386740669No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20540.1194974590791016No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20220.11763576546151093No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20090.11687945242936472No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18660.1085600090757564No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18570.10803640774580901No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18380.10693102716036454No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC18380.10693102716036454No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT18260.10623289205376804No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT17700.10297492822298436No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17660.10274221652078552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT5500.018.9084228
CCCTATA701.7886443E-518.2850672
GACTACG450.00884788417.7792151
CTAGCGG5850.017.77714729
TAGCGGC6150.017.6904330
ATCGTTT5950.017.47837329
TATTAGT753.2361833E-517.0660612
CGTTTAT6000.017.06606131
CCTATAC854.9857463E-616.9415633
TCTAGCG6200.016.773628
TCGTTTA6150.016.64981730
CGGCATC6350.016.37737325
ACCGTCG4800.016.335138
CGACCGT600.002440774316.001766
TAGTACG805.6251636E-515.99943352
TGTAGGA6500.015.9994332
GGCATCG6550.015.877326
GTCCTAC5850.015.8645311
TACCGTC5150.015.5356897
AACCGCG2800.015.4302697
Produced by FastQC (version 0.11.5)