##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1718865
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17555421746327	32.0	32.0	32.0	32.0	32.0
2	31.261898403888612	32.0	32.0	32.0	32.0	32.0
3	31.353544926448556	32.0	32.0	32.0	32.0	32.0
4	31.46287812015487	32.0	32.0	32.0	32.0	32.0
5	31.37527729053765	32.0	32.0	32.0	32.0	32.0
6	34.892695470557605	36.0	36.0	36.0	36.0	36.0
7	34.92015254252079	36.0	36.0	36.0	36.0	36.0
8	34.851319911685906	36.0	36.0	36.0	32.0	36.0
9	34.97357209553979	36.0	36.0	36.0	36.0	36.0
10	34.814849333717305	36.0	36.0	36.0	32.0	36.0
11	34.9973278878795	36.0	36.0	36.0	36.0	36.0
12	34.88826056729295	36.0	36.0	36.0	32.0	36.0
13	34.95254717502538	36.0	36.0	36.0	36.0	36.0
14	34.8910222734188	36.0	36.0	36.0	32.0	36.0
15	34.864877695456016	36.0	36.0	36.0	32.0	36.0
16	34.86493529160231	36.0	36.0	36.0	32.0	36.0
17	34.838035564165885	36.0	36.0	36.0	32.0	36.0
18	34.83855916549584	36.0	36.0	36.0	32.0	36.0
19	34.833504085544824	36.0	36.0	36.0	32.0	36.0
20	34.816488787659296	36.0	36.0	36.0	32.0	36.0
21	34.80337490146114	36.0	36.0	36.0	32.0	36.0
22	34.79241534384609	36.0	36.0	36.0	32.0	36.0
23	34.7306734385772	36.0	36.0	36.0	32.0	36.0
24	34.70273872584525	36.0	36.0	36.0	32.0	36.0
25	34.679216226986995	36.0	36.0	36.0	32.0	36.0
26	34.61541773204993	36.0	36.0	36.0	32.0	36.0
27	34.60339468195583	36.0	36.0	36.0	32.0	36.0
28	34.5653608631277	36.0	36.0	36.0	32.0	36.0
29	34.52250234893374	36.0	36.0	36.0	32.0	36.0
30	34.50588673339675	36.0	36.0	36.0	32.0	36.0
31	34.50652087278524	36.0	36.0	36.0	32.0	36.0
32	34.469880415274034	36.0	36.0	36.0	32.0	36.0
33	34.43719140246616	36.0	36.0	36.0	32.0	36.0
34	34.415120442850366	36.0	36.0	36.0	32.0	36.0
35	34.38427392494466	36.0	36.0	36.0	32.0	36.0
36	34.347885377851085	36.0	36.0	36.0	32.0	36.0
37	34.34185814476413	36.0	36.0	36.0	32.0	36.0
38	33.893504725502005	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	41.0
22	136.0
23	421.0
24	1144.0
25	2788.0
26	6074.0
27	11556.0
28	20469.0
29	32520.0
30	49178.0
31	71360.0
32	102531.0
33	164578.0
34	398520.0
35	857542.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92818393180057	18.389689094933217	12.062204286435751	26.61992268683046
2	15.715370317040605	20.55053770947689	36.84320758174726	26.89088439173524
3	17.90542014643384	24.461839644183808	28.82442774737981	28.808312462002544
4	11.790081798401266	15.909672690038745	36.234946417974705	36.06529909358528
5	14.098505814599536	37.087248954686906	33.119451637506884	15.694793593206672
6	34.49835035916268	36.012843403676506	16.328389937743584	13.16041629941723
7	30.18703708336412	30.61249423308629	20.712950524680064	18.487518158869527
8	28.2465549079544	33.10546409110426	19.21094796319805	19.437033037743294
9	27.489988614103034	13.718464061699112	18.324137927022043	40.46740939717581
10	15.726077637865274	26.467781984720034	31.32718814650709	26.478952230907606
11	38.122918562408806	20.758870909560592	22.086976455173602	19.031234072857007
12	24.814775382972076	23.707994663902042	28.511646388679935	22.96558356444595
13	29.621773450371876	19.000514293161064	25.331207122843924	26.04650513362314
14	23.531516436718416	19.898712231617957	24.80084241636196	31.768928915301668
15	25.202968237761546	27.50215985548603	22.1163965756473	25.178475331105123
16	25.866110329909947	25.551600098437167	23.81626691597876	24.766022655674128
17	23.947372248547733	25.83326788316709	25.303790582739193	24.915569285545985
18	25.181093337754856	24.24553411698999	26.65194765150259	23.92142489375256
19	25.78306033341769	24.63736244556728	25.38006184313486	24.19951537788017
20	25.795568587410877	23.96767634456458	25.091150264855006	25.145604803169537
21	27.181340223159147	23.99900399275568	24.142470924093427	24.677184859991748
22	25.83172713590321	24.25044927955282	25.0574944018226	24.86032918272137
23	24.124144856221815	23.865369142276027	25.782973977565405	26.227512023936754
24	24.79863165519107	24.9327899515087	25.33561390801488	24.932964485285346
25	24.912951278896248	24.367300515165528	25.432247442352946	25.287500763585275
26	24.408315952678077	25.380352732762606	25.949332844638757	24.26199846992056
27	25.42291037738319	24.605218682349904	24.92095065168079	25.05092028858612
28	24.215456129480792	24.655804847966536	25.951369072033	25.177369950519672
29	24.38196135240406	24.620083601679017	25.83687491455117	25.161080131365754
30	24.36572971117569	24.877346388459827	26.07522987552833	24.68169402483616
31	25.148106453968172	24.584885956721443	24.758314352785124	25.508693236525264
32	24.593729001404995	24.91079869565091	24.844592216375343	25.650880086568755
33	24.253039069385903	24.26775808454998	25.690964677272504	25.788238168791615
34	25.055079951014186	24.671629243716055	25.77689347330942	24.496397331960335
35	25.871083534774403	24.43984838832602	25.53423334584159	24.154834731057996
36	24.37509635718919	25.288722500021816	25.142288661413197	25.19389248137579
37	25.666820838169375	24.94425100284199	24.814397873015043	24.57453028597359
38	24.666684120044334	24.701998120853006	25.451097090231055	25.180220668871613
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	81.0
2	94.0
3	94.0
4	521.0
5	948.0
6	948.0
7	1246.5
8	1545.0
9	1553.0
10	1561.0
11	1561.0
12	2034.0
13	2507.0
14	3327.5
15	4148.0
16	4148.0
17	6248.0
18	8348.0
19	8348.0
20	9862.0
21	11376.0
22	12216.0
23	13056.0
24	13056.0
25	15588.5
26	18121.0
27	18121.0
28	23601.5
29	29082.0
30	35139.0
31	41196.0
32	41196.0
33	52918.0
34	64640.0
35	64640.0
36	70366.5
37	76093.0
38	86126.5
39	96160.0
40	96160.0
41	100350.0
42	104540.0
43	117646.0
44	130752.0
45	130752.0
46	134219.5
47	137687.0
48	137687.0
49	145843.5
50	154000.0
51	154740.0
52	155480.0
53	155480.0
54	147981.0
55	140482.0
56	140482.0
57	137649.0
58	134816.0
59	121456.0
60	108096.0
61	108096.0
62	103939.0
63	99782.0
64	83049.0
65	66316.0
66	66316.0
67	56174.0
68	46032.0
69	46032.0
70	37346.0
71	28660.0
72	22591.5
73	16523.0
74	16523.0
75	12531.5
76	8540.0
77	8540.0
78	8190.5
79	7841.0
80	6125.5
81	4410.0
82	4410.0
83	4147.0
84	3884.0
85	3884.0
86	2499.5
87	1115.0
88	910.0
89	705.0
90	705.0
91	421.0
92	137.0
93	107.0
94	77.0
95	77.0
96	53.0
97	29.0
98	29.0
99	23.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008203087502508923
2	0.0
3	0.0
4	2.9088962774854337E-4
5	1.1635585109941734E-4
6	3.4906755329825205E-4
7	4.6542340439766934E-4
8	0.002501650798637473
9	0.004188810639579025
10	7.563130321462128E-4
11	0.005875970480520576
12	3.4906755329825205E-4
13	5.817792554970867E-5
14	0.0
15	0.0
16	1.1635585109941734E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1635585109941734E-4
22	1.1635585109941734E-4
23	1.1635585109941734E-4
24	0.0
25	0.0
26	0.0
27	1.1635585109941734E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1718865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.811086951460226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44778745559141	34.10534064471928
2	14.678179369301288	13.448467027523819
3	4.833334554808446	6.642609286674809
4	2.0845484686412448	3.8198172460582933
5	1.0646812758327189	2.438710325138215
6	0.6503060071419787	1.7874735022942863
7	0.4034093380621816	1.2936434184098333
8	0.2879764183471934	1.055401019269869
9	0.21469559102919317	0.885189454986016
>10	1.1051255593756981	9.198295703564055
>50	0.09815067641103215	3.168368255474272
>100	0.10817761735229636	11.018927914135961
>500	0.015453772112133285	4.959540253505962
>1k	0.0080461788683008	5.867184213969331
>5k	1.277171248936635E-4	0.3110317342759797
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5316	0.30927385222225134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3835	0.22311234448313277	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2819	0.16400357212462874	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2509	0.14596841520421905	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2488	0.1447466787676752	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2483	0.14445578913992665	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2417	0.14061604605364586	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2398	0.1395106654682014	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2326	0.13532185482862238	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2234	0.12996948567804917	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2197	0.12781690243270996	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2166	0.126013386740669	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2054	0.1194974590791016	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2022	0.11763576546151093	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2009	0.11687945242936472	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1866	0.1085600090757564	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1857	0.10803640774580901	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1838	0.10693102716036454	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1838	0.10693102716036454	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1826	0.10623289205376804	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1770	0.10297492822298436	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1766	0.10274221652078552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8177925549708675E-5
2	0.0	0.0	0.0	0.0	5.8177925549708675E-5
3	0.0	0.0	0.0	0.0	5.8177925549708675E-5
4	0.0	0.0	0.0	0.0	5.8177925549708675E-5
5	0.0	0.0	0.0	0.0	5.8177925549708675E-5
6	0.0	0.0	0.0	0.0	1.1635585109941735E-4
7	0.0	0.0	0.0	0.0	1.1635585109941735E-4
8	0.0	0.0	0.0	0.0	1.1635585109941735E-4
9	0.0	0.0	0.0	0.0	1.1635585109941735E-4
10	0.0	0.0	0.0	0.0	1.1635585109941735E-4
11	0.0	0.0	0.0	0.0	1.7453377664912602E-4
12	0.0	0.0	0.0	0.0	6.399571810467954E-4
13	0.0	0.0	0.0	0.0	6.981351065965041E-4
14	0.0	0.0	0.0	0.0	6.981351065965041E-4
15	0.0	0.0	0.0	5.8177925549708675E-5	8.144909576959214E-4
16	0.0	0.0	0.0	1.1635585109941735E-4	8.144909576959214E-4
17	5.8177925549708675E-5	0.0	0.0	2.327117021988347E-4	8.144909576959214E-4
18	5.8177925549708675E-5	0.0	0.0	3.4906755329825205E-4	9.308468087953388E-4
19	5.8177925549708675E-5	0.0	0.0	3.4906755329825205E-4	9.308468087953388E-4
20	5.8177925549708675E-5	0.0	0.0	4.654234043976694E-4	9.890247343450475E-4
21	5.8177925549708675E-5	0.0	0.0	4.654234043976694E-4	9.890247343450475E-4
22	5.8177925549708675E-5	0.0	0.0	8.726688832456301E-4	0.0012217364365438822
23	5.8177925549708675E-5	0.0	0.0	0.0015708039898421342	0.0012217364365438822
24	5.8177925549708675E-5	0.0	0.0	0.0024434728730877643	0.0012217364365438822
25	5.8177925549708675E-5	0.0	0.0	0.002850718351935725	0.0012217364365438822
26	5.8177925549708675E-5	0.0	0.0	0.003548853458532229	0.0012217364365438822
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	550	0.0	18.90842	28
CCCTATA	70	1.7886443E-5	18.285067	2
GACTACG	45	0.008847884	17.779215	1
CTAGCGG	585	0.0	17.777147	29
TAGCGGC	615	0.0	17.69043	30
ATCGTTT	595	0.0	17.478373	29
TATTAGT	75	3.2361833E-5	17.066061	2
CGTTTAT	600	0.0	17.066061	31
CCTATAC	85	4.9857463E-6	16.941563	3
TCTAGCG	620	0.0	16.7736	28
TCGTTTA	615	0.0	16.649817	30
CGGCATC	635	0.0	16.377373	25
ACCGTCG	480	0.0	16.33513	8
CGACCGT	60	0.0024407743	16.00176	6
TAGTACG	80	5.6251636E-5	15.9994335	2
TGTAGGA	650	0.0	15.999433	2
GGCATCG	655	0.0	15.8773	26
GTCCTAC	585	0.0	15.864531	1
TACCGTC	515	0.0	15.535689	7
AACCGCG	280	0.0	15.430269	7
>>END_MODULE