FastQCFastQC Report
Thu 2 Feb 2017
SRR4062819_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062819_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607602
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT52020.3235875546310592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT37440.23289346492477614No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23070.1435056686916289No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20990.13056714286247467No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19720.12266717757255838No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18930.11775302593552385No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18690.11626011910908296No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18260.11358532771170973No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17740.11035069625442119No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17710.11016408290111608No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17580.10935542503679394No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17250.10730267815043774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16780.1043790689486577No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16380.10189089090458957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG550.001366123117.452347
CGTCGTA3850.016.62386110
ATACCGT4100.016.3856246
TACCGTC4250.015.8097677
ACCGTCG4050.015.405468
CCGTCGT4150.015.0365849
GTATAGG1501.9408617E-914.9505141
ATTATAC1501.9772415E-914.9314473
GACGAAA2900.014.896522522
GCATTCG4750.014.82102422
TAGCGTA2700.014.81294257
AATAGCG2850.014.5923775
CGAGCCG5600.014.57185415
GTCCTAT5750.014.4862131
TAAGGTA901.5380602E-414.2186564
ATAGCGT3050.014.1599436
GTCGTAG4550.014.06634411
CCTATTC6050.014.0147583
GTATACA1503.1242962E-813.882621
AGAACCG3350.013.8468425
Produced by FastQC (version 0.11.5)