##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062819_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1607602
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240026449332607	32.0	32.0	32.0	32.0	32.0
2	30.888147066251474	32.0	32.0	32.0	32.0	32.0
3	30.94361975165495	32.0	32.0	32.0	32.0	32.0
4	31.00446005914399	32.0	32.0	32.0	32.0	32.0
5	30.938955661911343	32.0	32.0	32.0	32.0	32.0
6	34.58235060668001	36.0	36.0	36.0	32.0	36.0
7	34.506810143306616	36.0	36.0	36.0	32.0	36.0
8	34.494144072973285	36.0	36.0	36.0	32.0	36.0
9	34.61280901616196	36.0	36.0	36.0	32.0	36.0
10	34.36286655527923	36.0	36.0	36.0	32.0	36.0
11	34.616755266539855	36.0	36.0	36.0	32.0	36.0
12	34.4456326876926	36.0	36.0	36.0	32.0	36.0
13	34.51331113049125	36.0	36.0	36.0	32.0	36.0
14	34.43023895217846	36.0	36.0	36.0	32.0	36.0
15	34.37465367671849	36.0	36.0	36.0	32.0	36.0
16	34.39181277455489	36.0	36.0	36.0	32.0	36.0
17	34.31705297704283	36.0	36.0	36.0	32.0	36.0
18	34.35033795678284	36.0	36.0	36.0	32.0	36.0
19	34.3355146360853	36.0	36.0	36.0	32.0	36.0
20	34.3109196181642	36.0	36.0	36.0	32.0	36.0
21	34.28891541563148	36.0	36.0	36.0	32.0	36.0
22	34.26548237685696	36.0	36.0	36.0	32.0	36.0
23	34.21970301106866	36.0	36.0	36.0	32.0	36.0
24	34.20631039274647	36.0	36.0	36.0	32.0	36.0
25	34.17641493354699	36.0	36.0	36.0	32.0	36.0
26	34.13249983515821	36.0	36.0	36.0	32.0	36.0
27	34.13484369887572	36.0	36.0	36.0	32.0	36.0
28	34.12160285941421	36.0	36.0	36.0	32.0	36.0
29	34.083159264544335	36.0	36.0	36.0	32.0	36.0
30	34.05702468645846	36.0	36.0	36.0	32.0	36.0
31	34.07377634514015	36.0	36.0	36.0	32.0	36.0
32	34.017547875655794	36.0	36.0	36.0	32.0	36.0
33	33.980199701169816	36.0	36.0	36.0	32.0	36.0
34	33.990935567385456	36.0	36.0	36.0	32.0	36.0
35	33.93772152560149	36.0	36.0	36.0	32.0	36.0
36	33.89189301829682	36.0	36.0	36.0	32.0	36.0
37	33.890378961957005	36.0	36.0	36.0	32.0	36.0
38	33.298802813134095	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	5.0
8	4.0
9	10.0
10	11.0
11	19.0
12	10.0
13	11.0
14	270.0
15	521.0
16	609.0
17	731.0
18	933.0
19	1131.0
20	1582.0
21	2107.0
22	3153.0
23	4487.0
24	6778.0
25	10072.0
26	14702.0
27	21310.0
28	30051.0
29	40645.0
30	54730.0
31	74083.0
32	101399.0
33	151752.0
34	341607.0
35	744877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3058059694521	17.367400003113957	11.684369501922868	25.642424525511075
2	16.45762562068137	20.007630537585687	37.756659293384836	25.778084548348108
3	18.393959509001697	24.590290397214417	28.835729825266466	28.18002026851742
4	12.356832359183754	15.747569602551948	36.47101562869349	35.4245824095708
5	14.411687498989176	36.802297589322706	33.657668148380374	15.128346763307743
6	33.39622218987583	36.04072659956034	17.22582386890255	13.337227341661286
7	29.752967309674478	30.92315817171052	21.30118107664775	18.022693441967256
8	28.157579783925275	32.64895570517893	19.852052013197376	19.34141249769842
9	27.829210715385404	14.140377396048134	18.68449453998053	39.34591734858593
10	15.777330592161535	26.67616331329292	31.902076717560814	25.644429376984736
11	36.951764344356626	21.395513465699075	22.44038502929273	19.21233716065157
12	24.69727149331963	24.00659141324727	29.012357966739366	22.28377912669373
13	29.549895821603283	19.631639623004233	25.269778186182258	25.548686369210227
14	23.834174005439692	19.941596721254363	25.449035860658288	30.775193412647656
15	25.159067236940185	26.834919753954438	22.984329092784765	25.021683916320615
16	25.50299704805542	25.868117443224058	24.027335815579672	24.60154969314085
17	23.807538748030012	25.879036337380224	25.58891219311579	24.724512721473975
18	24.81164934274003	24.702536287555823	26.591423863162028	23.894390506542116
19	25.301904868550384	25.31577768653652	25.901048859691688	23.481268585221404
20	25.601261590238227	24.09544042127658	25.63951987408981	24.66377811439538
21	26.95225309740239	24.401110809741482	24.601735889058375	24.044900203797756
22	25.603837085606575	24.473427186821528	25.213036635209367	24.709699092362534
23	24.15416381273745	24.232674083461024	25.796036288915307	25.81712581488622
24	24.482492819010446	25.344895913757203	25.508579548317776	24.664031718914575
25	24.762275091456587	24.529091904536745	25.543824502899238	25.164808501107434
26	24.419621108849856	25.333904903286257	26.01026959395098	24.236204393912907
27	25.258454758441694	25.018321079879907	25.037855270312136	24.68536889136626
28	24.28842658199657	24.81203662346485	26.116924853218737	24.782611941319843
29	24.213239401921346	25.285458506644837	26.00257307700443	24.498729014429387
30	24.358028500548663	25.193337928323135	26.247365553003704	24.201268018124498
31	24.74103925204808	25.14614462485762	24.98869350406816	25.12412261902614
32	24.312245641931025	25.213346621229032	25.018693879480775	25.455713857359168
33	24.043350405826835	24.778299166572832	25.611353073390987	25.56699735420935
34	24.852322433863158	24.95030754883547	25.882503715657773	24.314866301643598
35	25.639154393122958	24.771249515497544	25.652468489453558	23.93712760192594
36	24.33866219387463	25.6205252378452	25.16150827325508	24.879304295025097
37	25.31303015428948	25.44939664670316	25.12764140091687	24.109931798090496
38	24.234050521250005	25.347966571320864	25.62442378826015	24.793559119168986
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	546.0
1	483.0
2	420.0
3	420.0
4	962.5
5	1505.0
6	1505.0
7	1716.0
8	1927.0
9	1873.5
10	1820.0
11	1820.0
12	2336.0
13	2852.0
14	3640.0
15	4428.0
16	4428.0
17	6682.0
18	8936.0
19	8936.0
20	10446.0
21	11956.0
22	12891.5
23	13827.0
24	13827.0
25	15763.5
26	17700.0
27	17700.0
28	22509.5
29	27319.0
30	32937.5
31	38556.0
32	38556.0
33	47744.0
34	56932.0
35	56932.0
36	63043.5
37	69155.0
38	79112.5
39	89070.0
40	89070.0
41	95807.0
42	102544.0
43	115452.0
44	128360.0
45	128360.0
46	133756.5
47	139153.0
48	139153.0
49	144800.5
50	150448.0
51	149058.0
52	147668.0
53	147668.0
54	140261.5
55	132855.0
56	132855.0
57	128392.0
58	123929.0
59	111060.0
60	98191.0
61	98191.0
62	92151.5
63	86112.0
64	71091.5
65	56071.0
66	56071.0
67	46504.0
68	36937.0
69	36937.0
70	29756.5
71	22576.0
72	17617.5
73	12659.0
74	12659.0
75	9610.0
76	6561.0
77	6561.0
78	6224.0
79	5887.0
80	4695.0
81	3503.0
82	3503.0
83	3498.5
84	3494.0
85	3494.0
86	2353.0
87	1212.0
88	1020.5
89	829.0
90	829.0
91	626.5
92	424.0
93	356.5
94	289.0
95	289.0
96	272.0
97	255.0
98	255.0
99	475.5
100	696.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11986797727298175
2	0.05598400599153273
3	0.011383414551611655
4	0.0028614047506783395
5	2.4881780440681213E-4
6	2.4881780440681213E-4
7	6.220445110170303E-5
8	6.220445110170303E-4
9	4.354311577119212E-4
10	0.0010574756687289515
11	0.002239360239661309
12	0.00348344926169537
13	0.016359770639747896
14	0.00982830327406908
15	0.026125869462715272
16	0.011632232356018467
17	0.022331397945511387
18	0.0059094228546617885
19	0.00883303205644183
20	0.006655876267882225
21	0.007837760838814583
22	0.0082109875454248
23	0.010512552236187812
24	0.014493637106696806
25	0.017541655210680258
26	0.020714082216867113
27	0.010201529980679297
28	0.0067180807189839275
29	0.01169443680712017
30	0.0033590403594919637
31	0.007402329681102661
32	0.007713351936611176
33	0.008895236507543534
34	0.013373956986866152
35	0.01760385966178196
36	0.01579993057983257
37	0.010512552236187812
38	0.007464534132204364
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1607602.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.44277051025775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16129762232814	43.09760359746623
2	12.805398291434205	13.943227209459986
3	3.746403784820593	6.118938044872458
4	1.4828101212777243	3.229131645720424
5	0.7782121874934083	2.118401376599465
6	0.44682751775003204	1.459591680391987
7	0.30156494272706097	1.149262167957988
8	0.2136027191542206	0.9303299055424217
9	0.1508784144689021	0.739281500449379
>10	0.7451806554375013	7.366550682396713
>50	0.07357879499587607	2.8278930028527447
>100	0.07928561347756134	9.261175764594704
>500	0.010586606049252646	3.899416808295888
>1k	0.004257656780677695	3.533300267838629
>5k	1.1507180488318093E-4	0.3258963455610303
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5202	0.3235875546310592	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3744	0.23289346492477614	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2307	0.1435056686916289	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2099	0.13056714286247467	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1972	0.12266717757255838	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1893	0.11775302593552385	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1869	0.11626011910908296	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1826	0.11358532771170973	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1774	0.11035069625442119	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1771	0.11016408290111608	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1758	0.10935542503679394	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1725	0.10730267815043774	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1678	0.1043790689486577	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1638	0.10189089090458957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.220445110170303E-5
7	0.0	0.0	0.0	6.220445110170303E-5	6.220445110170303E-5
8	0.0	0.0	0.0	6.220445110170303E-5	1.2440890220340606E-4
9	0.0	0.0	0.0	6.220445110170303E-5	1.866133533051091E-4
10	0.0	0.0	0.0	6.220445110170303E-5	1.866133533051091E-4
11	0.0	0.0	0.0	6.220445110170303E-5	1.866133533051091E-4
12	0.0	0.0	0.0	6.220445110170303E-5	4.354311577119212E-4
13	0.0	0.0	0.0	6.220445110170303E-5	4.354311577119212E-4
14	0.0	0.0	0.0	6.220445110170303E-5	4.976356088136243E-4
15	0.0	0.0	0.0	6.220445110170303E-5	6.220445110170303E-4
16	0.0	0.0	0.0	1.866133533051091E-4	6.842489621187334E-4
17	0.0	0.0	0.0	2.4881780440681213E-4	6.842489621187334E-4
18	0.0	0.0	0.0	3.1102225550851516E-4	6.842489621187334E-4
19	0.0	0.0	0.0	5.598400599153273E-4	6.842489621187334E-4
20	0.0	0.0	0.0	5.598400599153273E-4	7.464534132204364E-4
21	0.0	0.0	0.0	6.842489621187334E-4	7.464534132204364E-4
22	0.0	0.0	0.0	8.708623154238425E-4	7.464534132204364E-4
23	0.0	0.0	0.0	0.0016795201797459819	7.464534132204364E-4
24	0.0	0.0	0.0	0.0028614047506783395	8.086578643221394E-4
25	0.0	0.0	0.0	0.0034212448105936668	8.086578643221394E-4
26	0.0	0.0	0.0	0.004229902674915806	8.708623154238425E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAG	55	0.0013661231	17.45234	7
CGTCGTA	385	0.0	16.623861	10
ATACCGT	410	0.0	16.385624	6
TACCGTC	425	0.0	15.809767	7
ACCGTCG	405	0.0	15.40546	8
CCGTCGT	415	0.0	15.036584	9
GTATAGG	150	1.9408617E-9	14.950514	1
ATTATAC	150	1.9772415E-9	14.931447	3
GACGAAA	290	0.0	14.8965225	22
GCATTCG	475	0.0	14.821024	22
TAGCGTA	270	0.0	14.8129425	7
AATAGCG	285	0.0	14.592377	5
CGAGCCG	560	0.0	14.571854	15
GTCCTAT	575	0.0	14.486213	1
TAAGGTA	90	1.5380602E-4	14.218656	4
ATAGCGT	305	0.0	14.159943	6
GTCGTAG	455	0.0	14.066344	11
CCTATTC	605	0.0	14.014758	3
GTATACA	150	3.1242962E-8	13.88262	1
AGAACCG	335	0.0	13.846842	5
>>END_MODULE