FastQCFastQC Report
Thu 2 Feb 2017
SRR4062819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607602
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT59060.3673794882066581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44290.2755035139294427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26780.16658352005036073No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24000.1492906826440873No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC21700.13498365889069558No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21370.13293091200433937No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19060.118561683799846No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18920.11769082148442214No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18700.11632232356018468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18230.11339871435840462No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17530.10904440278128541No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17430.10842235827026839No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17090.10630740693281049No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16790.10444127339975941No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16780.1043790689486577No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16410.10207750425789466No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16140.10039798407814869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC3550.018.47844330
CTAGCGG3550.018.47844329
TCTAGCG3700.017.72931728
TACCGTC3750.016.6411657
ATACCGT3850.016.624546
GTATAAT2250.016.35671
AGCGGCG4200.015.99962631
TAGCGTA3650.015.7819257
ATAGCGT3650.015.7819256
ACCGTCG3650.015.7819258
CGACGGT5300.015.3973057
AATAGCG3950.015.3934815
TACGACG5250.015.23916155
ACGACGG5500.014.8374026
TCGCGTA1951.8189894E-1214.7702669
TCGCCAA650.00416318514.76888728
TAAAACG650.00416318514.7688874
CGTCGTA4050.014.61830710
GTATTAG3950.014.5832981
AAACGGC3650.014.46541522
Produced by FastQC (version 0.11.5)