FastQCFastQC Report
Thu 2 Feb 2017
SRR4062818_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062818_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124401
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37550.33395559057667146No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26020.23141210297749648No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25670.22829933449009737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24400.21700443169296366No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23630.21015634102068567No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23160.2059763376233212No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22590.2009069718009856No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22530.2003733543460029No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21790.1937920724012163No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21040.18712185421393257No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20880.18569887433397872No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20230.17991801857166614No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19670.17493758899182765No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19140.17022396813948049No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT18180.16168608885975733No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17640.15688353176491304No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17440.15510480691497072No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17300.1538596995200111No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17300.1538596995200111No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC17040.15154735721508608No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA16880.15012437733513223No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT16420.14603331018026486No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC16100.14318735042035713No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT16050.14274266920787157No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT15720.13980777320546672No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15550.13829585708301576No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15140.13464947114063397No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14630.13011372277328107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14400.1280681891958474No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14140.12575584689092237No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14090.12531116567843678No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13850.12317669585850599No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT13650.12139797100856368No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG13570.12068648106858673No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC13390.11908562870363865No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13170.11712903136870209No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13010.11570605148874824No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT12400.11028094069642413No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA12340.10974732324144144No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG11870.10556731984407697No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC11640.10352178626664331No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT11510.1023656151141808No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC11410.10147625268920964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.10120944396171827No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC11270.10023114529425001No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11260.10014220905175289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG200.003758747131.9908395
TAGGACG1001.4551915E-1020.7940444
CCTATAC555.8420104E-520.359623
TACACCG400.00449085419.9942745
GTATTAT659.373651E-619.7068271
TTAGGAC1251.4915713E-1017.9164643
GATATAC1352.3646862E-1117.7908841
TCTAGCG5000.016.63967728
GGACTGT1354.4383341E-1016.5878436
CTAGCGG4950.016.48452829
GTATTAC1001.304239E-616.0117971
CACGCGG600.002442177615.9996925
ACGCGGC600.002442177615.9996926
ACCGAGT600.00244290315.9989788
CTAGACA703.7043737E-415.995424
AAGACGG5300.015.995425
TAGCGGC5150.015.84505730
ATACCGT4800.015.6621826
CAAGACG5350.015.546954
TCCTACA1357.887138E-915.4105352
Produced by FastQC (version 0.11.5)