##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062818_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1124401
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.235364429594068	32.0	32.0	32.0	32.0	32.0
2	30.895963272889297	32.0	32.0	32.0	32.0	32.0
3	30.94689439088012	32.0	32.0	32.0	32.0	32.0
4	31.003724649835778	32.0	32.0	32.0	32.0	32.0
5	30.947587204209174	32.0	32.0	32.0	32.0	32.0
6	34.589766462320824	36.0	36.0	36.0	32.0	36.0
7	34.50089603264316	36.0	36.0	36.0	32.0	36.0
8	34.49909863118229	36.0	36.0	36.0	32.0	36.0
9	34.59013465836477	36.0	36.0	36.0	32.0	36.0
10	34.35547371444885	36.0	36.0	36.0	32.0	36.0
11	34.59109161233403	36.0	36.0	36.0	32.0	36.0
12	34.41717501140607	36.0	36.0	36.0	32.0	36.0
13	34.507966463921676	36.0	36.0	36.0	32.0	36.0
14	34.407024718049875	36.0	36.0	36.0	32.0	36.0
15	34.353047533753525	36.0	36.0	36.0	32.0	36.0
16	34.364129878931095	36.0	36.0	36.0	32.0	36.0
17	34.27399299716026	36.0	36.0	36.0	32.0	36.0
18	34.30872793602994	36.0	36.0	36.0	32.0	36.0
19	34.299404749728964	36.0	36.0	36.0	32.0	36.0
20	34.278559873212494	36.0	36.0	36.0	32.0	36.0
21	34.246357838529136	36.0	36.0	36.0	32.0	36.0
22	34.22327977296356	36.0	36.0	36.0	32.0	36.0
23	34.17217078248774	36.0	36.0	36.0	32.0	36.0
24	34.14699026414954	36.0	36.0	36.0	32.0	36.0
25	34.13061976999309	36.0	36.0	36.0	32.0	36.0
26	34.082582637333125	36.0	36.0	36.0	32.0	36.0
27	34.08786900758715	36.0	36.0	36.0	32.0	36.0
28	34.074938567290495	36.0	36.0	36.0	32.0	36.0
29	34.03232743478528	36.0	36.0	36.0	32.0	36.0
30	34.01125221340074	36.0	36.0	36.0	32.0	36.0
31	34.03299534596643	36.0	36.0	36.0	32.0	36.0
32	33.97392389369985	36.0	36.0	36.0	32.0	36.0
33	33.925043645461	36.0	36.0	36.0	32.0	36.0
34	33.93357085239163	36.0	36.0	36.0	32.0	36.0
35	33.88607089463635	36.0	36.0	36.0	32.0	36.0
36	33.847379182337974	36.0	36.0	36.0	32.0	36.0
37	33.83875325617818	36.0	36.0	36.0	32.0	36.0
38	33.21668248249512	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	13.0
11	9.0
12	6.0
13	8.0
14	232.0
15	455.0
16	528.0
17	597.0
18	732.0
19	865.0
20	1216.0
21	1679.0
22	2312.0
23	3534.0
24	5107.0
25	7409.0
26	10784.0
27	15253.0
28	21226.0
29	28541.0
30	38566.0
31	51559.0
32	70359.0
33	105491.0
34	239808.0
35	518104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.727662825559854	17.427185633114785	11.304981981733297	25.54016955959207
2	16.961069425064785	20.22738274909588	36.77892829683629	26.03261952900305
3	18.384555720801814	24.73030861518099	28.54165628935964	28.343479374657548
4	12.306091411190266	15.393420315376611	36.046675026903955	36.25381324652916
5	14.809040546922317	36.534030119147886	32.80849092094368	15.848438412986116
6	33.9312538187135	35.8307608433676	16.93031909547962	13.30766624243928
7	30.166461965081854	30.59771380495037	21.186836368875518	18.04898786109226
8	28.697527570259695	31.509694059053718	19.85112059765208	19.941657773034507
9	28.01327642589508	13.830588607780689	18.814940248079196	39.341194718245035
10	16.726995245455793	25.49835556448873	30.14759898185518	27.6270502082003
11	38.23824998977216	21.01776798276742	21.787435231086942	18.956546796373473
12	25.4123657243282	23.86002422698659	27.83417499284041	22.893435055844805
13	29.394419454434022	18.655396107725576	25.45624682559661	26.49393761224379
14	24.358909360977325	20.152095313062855	24.333471197505993	31.15552412845383
15	25.561477156172803	26.690880883311852	22.385874269325782	25.36176769118957
16	26.475200374286985	25.215450723179732	23.351131533233534	24.958217369299753
17	24.270264211767927	25.52425158382955	24.841350182445066	25.364134021957458
18	25.914699496856354	23.506867639511853	26.360920405112015	24.217512458519778
19	25.989981446464245	24.616521719021556	25.198565525289197	24.194931309225005
20	26.067953781964793	23.282366366248844	24.738976255044147	25.91070359674222
21	28.384408422831477	23.59673581926934	23.63462521957619	24.384230538322992
22	26.55103467536649	23.552850893967346	24.616966434643704	25.27914799602246
23	24.082420089496562	23.22063099434398	25.655947892401333	27.041001023758128
24	25.08841528499253	24.9935067245428	24.710026328897744	25.208051661566927
25	25.114837217576948	23.858566091442803	24.938534068671057	26.0880626223092
26	24.802166912771977	25.40411677845185	25.569214894411928	24.224501414364248
27	26.10676811170102	24.068053797651313	24.358549018177744	25.46662907246993
28	24.49730329723499	24.114143814660384	25.86539966700346	25.523153221101165
29	24.372324367788078	24.44730655618283	25.823403159569747	25.356965916459345
30	24.172022277443467	24.873217439872782	26.291172091649873	24.66358819103388
31	25.418650073643988	24.793387694694076	23.95164044656008	25.836321785101855
32	24.77673191197176	24.520221538155027	24.087782759549984	26.615263790323223
33	24.305191749910833	23.820980643118446	25.234478605856253	26.63934900111447
34	25.75786821129671	24.215758393010738	25.228087621938037	24.798285773754515
35	26.84451165273083	23.740348692403487	25.284113147126845	24.131026507738838
36	24.584736520948635	25.169939140350785	24.52202670993199	25.72329762876859
37	26.102908802723885	25.034221993334384	24.50196882085109	24.360900383090645
38	24.614991901822155	24.44128798828099	25.243456752350518	25.700263357546337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	832.0
1	617.5
2	403.0
3	403.0
4	786.5
5	1170.0
6	1170.0
7	1308.0
8	1446.0
9	1377.5
10	1309.0
11	1309.0
12	1700.0
13	2091.0
14	2530.0
15	2969.0
16	2969.0
17	4339.0
18	5709.0
19	5709.0
20	6674.5
21	7640.0
22	7749.0
23	7858.0
24	7858.0
25	9034.0
26	10210.0
27	10210.0
28	13068.0
29	15926.0
30	19502.0
31	23078.0
32	23078.0
33	30711.0
34	38344.0
35	38344.0
36	41824.5
37	45305.0
38	52620.0
39	59935.0
40	59935.0
41	62136.5
42	64338.0
43	75215.5
44	86093.0
45	86093.0
46	86411.0
47	86729.0
48	86729.0
49	94078.5
50	101428.0
51	102439.0
52	103450.0
53	103450.0
54	97717.5
55	91985.0
56	91985.0
57	91374.0
58	90763.0
59	80768.0
60	70773.0
61	70773.0
62	71124.5
63	71476.0
64	58748.0
65	46020.0
66	46020.0
67	39253.5
68	32487.0
69	32487.0
70	26361.0
71	20235.0
72	15632.5
73	11030.0
74	11030.0
75	8269.5
76	5509.0
77	5509.0
78	5858.0
79	6207.0
80	4973.0
81	3739.0
82	3739.0
83	3976.0
84	4213.0
85	4213.0
86	2747.0
87	1281.0
88	1099.5
89	918.0
90	918.0
91	670.5
92	423.0
93	369.0
94	315.0
95	315.0
96	261.5
97	208.0
98	208.0
99	382.0
100	556.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12219839719103771
2	0.05843111132060537
3	0.013607245102058784
4	0.0027570235174106035
5	0.0
6	3.5574496998846494E-4
7	0.0
8	8.893624249711624E-5
9	1.7787248499423247E-4
10	2.6680872749134874E-4
11	0.0016897886074452087
12	0.0031127684873990687
13	0.015474906194498227
14	0.009427241704694321
15	0.02383491298922715
16	0.011561711524625111
17	0.020188527046845386
18	0.0062255369747981374
19	0.007737453097249113
20	0.006937026914775067
21	0.006759154429780835
22	0.007737453097249113
23	0.009960859159677019
24	0.014318735042035714
25	0.017876184741920365
26	0.01965490959186269
27	0.010138731644671252
28	0.005780855762312556
29	0.011917456494613576
30	0.0031127684873990687
31	0.006492345702289486
32	0.007114899399769299
33	0.008715751764717393
34	0.014407671284532832
35	0.017876184741920365
36	0.015563842436995342
37	0.011028094069642414
38	0.006581281944786602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1124401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.65813489538133
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57339965379138	37.5940455002607
2	12.166595182997671	11.35341278531604
3	3.445619627391346	4.822985561189969
4	1.316006304888161	2.4560959878657656
5	0.6402062902990238	1.4935415726821755
6	0.37137130849014255	1.0396495564684405
7	0.24294487331698905	0.7934748269955781
8	0.1573294722535825	0.5872559791541445
9	0.13014542121244854	0.5465108357051982
>10	0.6793080519858252	6.0870219825249725
>50	0.10770135715979515	3.5733367765565065
>100	0.1357652948081285	14.238227440485455
>500	0.02329587324702941	7.640044018271711
>1k	0.010311288158521214	7.774397176523361
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3755	0.33395559057667146	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2602	0.23141210297749648	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2567	0.22829933449009737	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2440	0.21700443169296366	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2363	0.21015634102068567	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2316	0.2059763376233212	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2259	0.2009069718009856	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2253	0.2003733543460029	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2179	0.1937920724012163	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2104	0.18712185421393257	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2088	0.18569887433397872	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2023	0.17991801857166614	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1967	0.17493758899182765	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1914	0.17022396813948049	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1818	0.16168608885975733	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1764	0.15688353176491304	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1744	0.15510480691497072	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1730	0.1538596995200111	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1730	0.1538596995200111	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1704	0.15154735721508608	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1688	0.15012437733513223	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1642	0.14603331018026486	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1610	0.14318735042035713	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1605	0.14274266920787157	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1572	0.13980777320546672	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1555	0.13829585708301576	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1514	0.13464947114063397	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1463	0.13011372277328107	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1440	0.1280681891958474	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1414	0.12575584689092237	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1409	0.12531116567843678	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1385	0.12317669585850599	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1365	0.12139797100856368	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1357	0.12068648106858673	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1339	0.11908562870363865	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1317	0.11712903136870209	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1301	0.11570605148874824	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1240	0.11028094069642413	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1234	0.10974732324144144	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1187	0.10556731984407697	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1164	0.10352178626664331	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	1151	0.1023656151141808	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	1141	0.10147625268920964	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.10120944396171827	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1127	0.10023114529425001	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1126	0.10014220905175289	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.893624249711624E-5
2	0.0	0.0	0.0	0.0	8.893624249711624E-5
3	0.0	0.0	0.0	0.0	8.893624249711624E-5
4	0.0	0.0	0.0	0.0	8.893624249711624E-5
5	0.0	0.0	0.0	0.0	8.893624249711624E-5
6	0.0	0.0	0.0	0.0	8.893624249711624E-5
7	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
8	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
9	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
10	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
11	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
12	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
13	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
14	0.0	0.0	0.0	3.5574496998846494E-4	8.893624249711624E-5
15	0.0	0.0	0.0	4.446812124855812E-4	8.893624249711624E-5
16	0.0	0.0	0.0	4.446812124855812E-4	8.893624249711624E-5
17	0.0	0.0	0.0	4.446812124855812E-4	8.893624249711624E-5
18	0.0	0.0	0.0	5.336174549826975E-4	8.893624249711624E-5
19	0.0	0.0	0.0	7.114899399769299E-4	8.893624249711624E-5
20	0.0	0.0	0.0	7.114899399769299E-4	1.7787248499423247E-4
21	0.0	0.0	0.0	8.004261824740462E-4	1.7787248499423247E-4
22	0.0	0.0	0.0	0.0014229798799538598	1.7787248499423247E-4
23	0.0	0.0	0.0	0.002579151032416371	1.7787248499423247E-4
24	0.0	0.0	0.0	0.0037353221848788823	1.7787248499423247E-4
25	0.0	0.0	0.0	0.004446812124855812	1.7787248499423247E-4
26	0.0	0.0	0.0	0.005247238307329858	2.6680872749134874E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	20	0.0037587471	31.990839	5
TAGGACG	100	1.4551915E-10	20.794044	4
CCTATAC	55	5.8420104E-5	20.35962	3
TACACCG	40	0.004490854	19.994274	5
GTATTAT	65	9.373651E-6	19.706827	1
TTAGGAC	125	1.4915713E-10	17.916464	3
GATATAC	135	2.3646862E-11	17.790884	1
TCTAGCG	500	0.0	16.639677	28
GGACTGT	135	4.4383341E-10	16.587843	6
CTAGCGG	495	0.0	16.484528	29
GTATTAC	100	1.304239E-6	16.011797	1
CACGCGG	60	0.0024421776	15.99969	25
ACGCGGC	60	0.0024421776	15.99969	26
ACCGAGT	60	0.002442903	15.998978	8
CTAGACA	70	3.7043737E-4	15.99542	4
AAGACGG	530	0.0	15.99542	5
TAGCGGC	515	0.0	15.845057	30
ATACCGT	480	0.0	15.662182	6
CAAGACG	535	0.0	15.54695	4
TCCTACA	135	7.887138E-9	15.410535	2
>>END_MODULE