FastQCFastQC Report
Thu 2 Feb 2017
SRR4062818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124401
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42370.3768228594602815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33340.29651343248538553No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26790.2382601936497744No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25220.22429720357772714No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24560.21842741157291748No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24440.2173601766629521No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23220.20650995507830394No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC22730.20215207919594522No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22660.2015295254984654No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22160.1970827133736096No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21710.19308058246123935No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20480.18214142463409405No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19850.17653844135677574No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19600.17431503529434783No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19170.17049077686697184No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG18840.167555880864567No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18760.16684439092459008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18510.16462098486216215No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT17730.1576839579473871No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA17610.15661672303742172No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT17510.15572736061245054No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT17090.15199203842757167No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16890.15021331357762932No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16180.1438988403603341No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16110.14327628666285427No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15500.13785117587053017No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC15480.13767330338553596No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT15430.13722862217305037No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG15090.1342047899281484No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG14770.1313588301682407No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC14320.12735669925587045No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG14270.12691201804338487No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14110.12548903816343102No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.12451073949596274No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC13520.12024179985610116No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13420.11935243743112998No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT13230.1176626488236848No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT13210.11748477633869055No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC12860.1143720078512915No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC12610.11214860178886357No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT12570.1117928568188751No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA12430.11054774942391549No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC12390.11019200445392702No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC12200.10850221584648183No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC12090.10752391717901355No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT11810.10503370238909428No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG11690.10396646747912888No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG11580.1029881688116606No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT11420.10156518893170675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT400.004480897420.00194510
ACCGTGG701.7857074E-518.2874938
TCTAGCG5300.018.11255528
CTAGCGG5350.017.94327729
TACCGTC5300.017.8130557
GTCGATA450.00884519917.7795079
ATTGTCG450.00884519917.7795076
TCTAGAT3250.017.7224372
ACCGTCG5350.017.6465768
GGGCGTT550.001363731717.4562447
CTATAAG652.0973648E-417.233981
ATACCGT5500.017.1653066
TAGCGGC5500.017.16301530
AACGAAT854.985448E-616.94056531
TAACGAA2200.016.72666713
ATAATAC1151.841363E-816.695053
CGTCGTA5500.016.58343110
AGCGGCG5800.016.55112631
CCGTCGT5650.016.4263769
GTCGTAG5850.016.13688711
Produced by FastQC (version 0.11.5)