##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1124401
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19137567469257	32.0	32.0	32.0	32.0	32.0
2	31.316949202286374	32.0	32.0	32.0	32.0	32.0
3	31.391636080010603	32.0	32.0	32.0	32.0	32.0
4	31.490508279519496	32.0	32.0	32.0	32.0	32.0
5	31.414280136712794	32.0	32.0	32.0	32.0	32.0
6	34.939786606379755	36.0	36.0	36.0	36.0	36.0
7	34.962125611770176	36.0	36.0	36.0	36.0	36.0
8	34.90555771472989	36.0	36.0	36.0	36.0	36.0
9	35.0068205204371	36.0	36.0	36.0	36.0	36.0
10	34.84960703521253	36.0	36.0	36.0	32.0	36.0
11	35.02194501783617	36.0	36.0	36.0	36.0	36.0
12	34.911409719486194	36.0	36.0	36.0	32.0	36.0
13	34.966420342920365	36.0	36.0	36.0	36.0	36.0
14	34.91076048491597	36.0	36.0	36.0	32.0	36.0
15	34.88602375842782	36.0	36.0	36.0	32.0	36.0
16	34.89181439717681	36.0	36.0	36.0	32.0	36.0
17	34.84652984122213	36.0	36.0	36.0	32.0	36.0
18	34.84161789254901	36.0	36.0	36.0	32.0	36.0
19	34.84150583288346	36.0	36.0	36.0	32.0	36.0
20	34.8242406401275	36.0	36.0	36.0	32.0	36.0
21	34.80993791360911	36.0	36.0	36.0	32.0	36.0
22	34.79703148609793	36.0	36.0	36.0	32.0	36.0
23	34.72786754903277	36.0	36.0	36.0	32.0	36.0
24	34.69300987814845	36.0	36.0	36.0	32.0	36.0
25	34.67086475376667	36.0	36.0	36.0	32.0	36.0
26	34.60928263137439	36.0	36.0	36.0	32.0	36.0
27	34.59794059236874	36.0	36.0	36.0	32.0	36.0
28	34.55507421284755	36.0	36.0	36.0	32.0	36.0
29	34.513142553235014	36.0	36.0	36.0	32.0	36.0
30	34.49139141640749	36.0	36.0	36.0	32.0	36.0
31	34.487266553480474	36.0	36.0	36.0	32.0	36.0
32	34.461177106743946	36.0	36.0	36.0	32.0	36.0
33	34.407983450744	36.0	36.0	36.0	32.0	36.0
34	34.39266685106114	36.0	36.0	36.0	32.0	36.0
35	34.34179887780249	36.0	36.0	36.0	32.0	36.0
36	34.31913614448938	36.0	36.0	36.0	32.0	36.0
37	34.31666905312251	36.0	36.0	36.0	32.0	36.0
38	33.85800261650425	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	11.0
21	28.0
22	99.0
23	255.0
24	792.0
25	1944.0
26	4012.0
27	7968.0
28	13793.0
29	21434.0
30	32031.0
31	45988.0
32	65631.0
33	104679.0
34	254971.0
35	570763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02080053508946	18.660266228348714	12.035510158338804	26.283423078223034
2	15.952404880465243	21.242421520436213	35.99596585204033	26.80920774705821
3	17.334207280142937	25.348163155315582	29.02816699736126	28.289462567180212
4	11.74112725209401	16.166072867436622	36.18522978518283	35.907570095286545
5	14.247776591960157	36.982034863038066	32.73132337246531	16.038865172536465
6	33.83825492552021	36.37214191759331	16.46897587066513	13.32062728622135
7	29.677122132217704	30.87359523580828	21.3010099716468	18.14827266032722
8	28.47404113396331	31.816075597554196	19.73958866036687	19.97029460811562
9	27.90606872924364	13.457060676619642	18.7296883365915	39.90718225754522
10	16.481557901926745	25.801950223411218	30.151442923414063	27.565048951247974
11	38.28735841756756	20.851340113577237	21.83173314240736	19.029568326447844
12	25.130825794470983	24.035660034365115	27.876566618878044	22.95694755228585
13	29.43826780042992	18.60695110356929	25.42580600979903	26.52897508620176
14	24.29142272196485	20.059658431467064	24.37564534360962	31.273273502958464
15	25.556096090273844	26.828506911680083	22.260474688300704	25.354922309745366
16	26.401346138966435	25.186477066455826	23.280039772287644	25.13213702229009
17	24.227744372336915	25.561965882278653	24.81596868021284	25.39432106517159
18	25.87937933175086	23.3923662465615	26.41335253170355	24.31490188998409
19	25.994018148329644	24.539110157319318	25.216982197632337	24.249889496718698
20	26.04933649116285	23.129292841255033	24.861592972613863	25.959777694968256
21	28.37015297047314	23.547047314123088	23.72643187477766	24.35636784062611
22	26.467336830899296	23.497755693920585	24.75984991119716	25.275057563982955
23	23.985457106825166	22.997283879907293	25.803496626639323	27.213762386628222
24	24.99953308472689	24.77808184090907	24.96342497027306	25.258960104090978
25	25.157417289220916	23.66648879402348	25.13322660974742	26.042867307008184
26	24.85527849939657	25.10954721669582	25.74330688073027	24.291867403177335
27	26.14648220022128	23.82879341441983	24.44957114753165	25.57515323782724
28	24.41904622994821	23.91886880214443	26.178827660238653	25.483257307668705
29	24.476410106358852	24.10768044496581	26.049781172375337	25.366128276300003
30	24.271501003645497	24.536708878771897	26.481033012243856	24.710757105338754
31	25.274968627740456	24.384450031616833	24.286709101112503	26.0538722395302
32	24.905260667679947	24.29578059784721	24.237527359011597	26.561431375461247
33	24.275503134557866	23.549961268266394	25.4679602739592	26.70657532321654
34	25.63729487967371	23.834023626802182	25.622175718449203	24.906505775074905
35	26.880267804813407	23.49455398919069	25.38845127316678	24.236726932829125
36	24.72071796449843	24.821482727247663	24.75095628694745	25.706843021306458
37	26.285106470022708	24.782172908063938	24.587847218207738	24.344873403705616
38	24.742529348986125	23.93721095695482	25.544290288153682	25.775969405905368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	452.0
1	323.0
2	194.0
3	194.0
4	707.5
5	1221.0
6	1221.0
7	1340.5
8	1460.0
9	1383.5
10	1307.0
11	1307.0
12	1661.5
13	2016.0
14	2590.5
15	3165.0
16	3165.0
17	4736.5
18	6308.0
19	6308.0
20	7058.5
21	7809.0
22	7901.0
23	7993.0
24	7993.0
25	8908.5
26	9824.0
27	9824.0
28	12762.0
29	15700.0
30	19171.5
31	22643.0
32	22643.0
33	30831.5
34	39020.0
35	39020.0
36	42063.0
37	45106.0
38	52378.5
39	59651.0
40	59651.0
41	62229.0
42	64807.0
43	75365.0
44	85923.0
45	85923.0
46	86769.0
47	87615.0
48	87615.0
49	94691.5
50	101768.0
51	102811.0
52	103854.0
53	103854.0
54	97803.0
55	91752.0
56	91752.0
57	91690.5
58	91629.0
59	81153.5
60	70678.0
61	70678.0
62	71631.5
63	72585.0
64	59305.0
65	46025.0
66	46025.0
67	39432.0
68	32839.0
69	32839.0
70	26487.5
71	20136.0
72	15560.5
73	10985.0
74	10985.0
75	8133.0
76	5281.0
77	5281.0
78	5659.5
79	6038.0
80	4705.5
81	3373.0
82	3373.0
83	3430.0
84	3487.0
85	3487.0
86	2202.0
87	917.0
88	779.5
89	642.0
90	642.0
91	381.0
92	120.0
93	83.0
94	46.0
95	46.0
96	31.0
97	16.0
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009160432977202973
2	0.0
3	0.0
4	2.6680872749134874E-4
5	8.893624249711624E-5
6	1.7787248499423247E-4
7	0.001156171152462511
8	0.002312342304925022
9	0.004180003397364464
10	0.001511916122450976
11	0.004980429579838509
12	4.446812124855812E-4
13	3.5574496998846494E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.893624249711624E-5
22	0.0
23	2.6680872749134874E-4
24	0.0
25	8.893624249711624E-5
26	0.0
27	4.446812124855812E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.893624249711624E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1124401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.49728252065436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03410805247584	35.16803034778941
2	13.181168369695309	11.730523457972902
3	3.6929689209244736	4.92981244243117
4	1.426808607542243	2.539564228508345
5	0.6778749805768823	1.508179726220631
6	0.4035630661888021	1.0774475862662798
7	0.26516787354270016	0.8259474849101475
8	0.1823917301337224	0.6492749076152953
9	0.11846293255814708	0.4744150720438585
>10	0.7224899043887018	6.23135886302727
>50	0.11346234395094452	3.6198023359613156
>100	0.1439815457819954	14.455392510496386
>500	0.02449895194290287	7.5491078880139675
>1k	0.01305272029744825	9.24114314874301
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4237	0.3768228594602815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3334	0.29651343248538553	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2679	0.2382601936497744	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2522	0.22429720357772714	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2456	0.21842741157291748	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2444	0.2173601766629521	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2322	0.20650995507830394	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2273	0.20215207919594522	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2266	0.2015295254984654	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2216	0.1970827133736096	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2171	0.19308058246123935	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2048	0.18214142463409405	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1985	0.17653844135677574	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1960	0.17431503529434783	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1917	0.17049077686697184	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1884	0.167555880864567	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1876	0.16684439092459008	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1851	0.16462098486216215	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1773	0.1576839579473871	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1761	0.15661672303742172	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1751	0.15572736061245054	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1709	0.15199203842757167	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1689	0.15021331357762932	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1618	0.1438988403603341	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1611	0.14327628666285427	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1550	0.13785117587053017	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1548	0.13767330338553596	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1543	0.13722862217305037	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1509	0.1342047899281484	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1477	0.1313588301682407	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1432	0.12735669925587045	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1427	0.12691201804338487	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1411	0.12548903816343102	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1400	0.12451073949596274	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	1352	0.12024179985610116	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1342	0.11935243743112998	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1323	0.1176626488236848	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1321	0.11748477633869055	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	1286	0.1143720078512915	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1261	0.11214860178886357	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1257	0.1117928568188751	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	1243	0.11054774942391549	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	1239	0.11019200445392702	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTC	1220	0.10850221584648183	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	1209	0.10752391717901355	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	1181	0.10503370238909428	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	1169	0.10396646747912888	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1158	0.1029881688116606	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGAT	1142	0.10156518893170675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	8.893624249711624E-5	0.0
8	0.0	0.0	0.0	8.893624249711624E-5	0.0
9	0.0	0.0	0.0	8.893624249711624E-5	0.0
10	0.0	0.0	0.0	8.893624249711624E-5	0.0
11	0.0	0.0	0.0	8.893624249711624E-5	0.0
12	0.0	0.0	0.0	8.893624249711624E-5	0.0
13	0.0	0.0	0.0	8.893624249711624E-5	0.0
14	0.0	0.0	0.0	8.893624249711624E-5	0.0
15	0.0	0.0	0.0	1.7787248499423247E-4	8.893624249711624E-5
16	0.0	0.0	0.0	1.7787248499423247E-4	1.7787248499423247E-4
17	0.0	0.0	0.0	1.7787248499423247E-4	1.7787248499423247E-4
18	0.0	0.0	0.0	2.6680872749134874E-4	1.7787248499423247E-4
19	0.0	0.0	0.0	3.5574496998846494E-4	1.7787248499423247E-4
20	0.0	0.0	0.0	3.5574496998846494E-4	2.6680872749134874E-4
21	0.0	0.0	0.0	4.446812124855812E-4	2.6680872749134874E-4
22	0.0	0.0	0.0	0.001067234909965395	2.6680872749134874E-4
23	0.0	0.0	0.0	0.002223406062427906	2.6680872749134874E-4
24	0.0	0.0	0.0	0.0035574496998846497	2.6680872749134874E-4
25	0.0	0.0	0.0	0.00426893963986158	2.6680872749134874E-4
26	0.0	0.0	0.0	0.005158302064832742	2.6680872749134874E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	40	0.0044808974	20.001945	10
ACCGTGG	70	1.7857074E-5	18.287493	8
TCTAGCG	530	0.0	18.112555	28
CTAGCGG	535	0.0	17.943277	29
TACCGTC	530	0.0	17.813055	7
GTCGATA	45	0.008845199	17.779507	9
ATTGTCG	45	0.008845199	17.779507	6
TCTAGAT	325	0.0	17.722437	2
ACCGTCG	535	0.0	17.646576	8
GGGCGTT	55	0.0013637317	17.456244	7
CTATAAG	65	2.0973648E-4	17.23398	1
ATACCGT	550	0.0	17.165306	6
TAGCGGC	550	0.0	17.163015	30
AACGAAT	85	4.985448E-6	16.940565	31
TAACGAA	220	0.0	16.726667	13
ATAATAC	115	1.841363E-8	16.69505	3
CGTCGTA	550	0.0	16.583431	10
AGCGGCG	580	0.0	16.551126	31
CCGTCGT	565	0.0	16.426376	9
GTCGTAG	585	0.0	16.136887	11
>>END_MODULE