FastQCFastQC Report
Thu 2 Feb 2017
SRR4062817_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062817_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439477
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48890.33963724324876327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT33740.234390684950159No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23310.1619338134614169No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC23120.16061388962796905No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22500.1563067697504024No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21540.14963768090771856No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG21260.1476925299952691No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC20880.14505268232837343No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20410.14178760758247613No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20180.14018980504724982No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC20150.13998139602091594No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20030.13914775991558045No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18940.1315755652921165No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18750.13025564145866866No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18540.12879677827433159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18410.1278936724935515No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17480.1214329926772015No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG17170.11927943273841819No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG17050.1184457966330827No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16980.11795950890497034No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16500.11462496448362842No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA16440.1142081464309607No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15840.11003996590428329No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15750.10941473882528167No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA15470.10746958791283223No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15170.10538549764949354No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15020.10434345251782418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG400.00448976919.9955715
ATCGTTT3900.018.87023729
CCGTCTA450.00885022117.7781649
CATCGTT4050.017.77569428
GCATCGT4200.017.14084827
GGCATCG4300.017.11486626
TAGACCG753.2407603E-517.0628875
ATACCGT4750.016.8383756
AAGACGG6300.016.7581925
CGTTTAT4300.016.74280531
TGCACCG1151.84682E-816.6919565
CGGCATC4800.016.66529325
CGCAAGA6000.016.5394382
TACCGTC4650.016.5147677
CAAGACG6250.016.3803714
CTAGCGG5300.016.30054329
ACCGTCG4950.016.1602828
AACCGCG2600.015.9986797
TCTAGCG5500.015.99812428
TATCCCG703.7034455E-415.9964575
Produced by FastQC (version 0.11.5)