##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062817_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1439477
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.182224516265283	32.0	32.0	32.0	32.0	32.0
2	30.752877607631106	32.0	32.0	32.0	32.0	32.0
3	30.81239992024881	32.0	32.0	32.0	32.0	32.0
4	30.883159647566444	32.0	32.0	32.0	32.0	32.0
5	30.766745838940114	32.0	32.0	32.0	32.0	32.0
6	34.42182542687379	36.0	36.0	36.0	32.0	36.0
7	34.34781938162263	36.0	36.0	36.0	32.0	36.0
8	34.316125926291285	36.0	36.0	36.0	32.0	36.0
9	34.47726639605912	36.0	36.0	36.0	32.0	36.0
10	34.163391981949	36.0	36.0	36.0	32.0	36.0
11	34.47973743241469	36.0	36.0	36.0	32.0	36.0
12	34.26493997472693	36.0	36.0	36.0	32.0	36.0
13	34.37996647393463	36.0	36.0	36.0	32.0	36.0
14	34.26464750739331	36.0	36.0	36.0	32.0	36.0
15	34.194201088311935	36.0	36.0	36.0	32.0	36.0
16	34.210896040714786	36.0	36.0	36.0	32.0	36.0
17	34.11645271164458	36.0	36.0	36.0	32.0	36.0
18	34.15253942925104	36.0	36.0	36.0	32.0	36.0
19	34.14207938021934	36.0	36.0	36.0	32.0	36.0
20	34.121868567542236	36.0	36.0	36.0	32.0	36.0
21	34.09690950254849	36.0	36.0	36.0	32.0	36.0
22	34.07355379766401	36.0	36.0	36.0	32.0	36.0
23	34.01114224124456	36.0	36.0	36.0	32.0	36.0
24	33.99016448335055	36.0	36.0	36.0	32.0	36.0
25	33.96769382213123	36.0	36.0	36.0	32.0	36.0
26	33.911606090267504	36.0	36.0	36.0	32.0	36.0
27	33.913986816044996	36.0	36.0	36.0	32.0	36.0
28	33.89631859348916	36.0	36.0	36.0	32.0	36.0
29	33.8586931225716	36.0	36.0	36.0	32.0	36.0
30	33.823385854723625	36.0	36.0	36.0	32.0	36.0
31	33.84212807846183	36.0	36.0	36.0	32.0	36.0
32	33.78732206211006	36.0	36.0	36.0	32.0	36.0
33	33.73786798955454	36.0	36.0	36.0	32.0	36.0
34	33.7436819066925	36.0	36.0	36.0	32.0	36.0
35	33.695476204204724	36.0	36.0	36.0	27.0	36.0
36	33.649592178270304	36.0	36.0	36.0	27.0	36.0
37	33.6453420235266	36.0	36.0	36.0	27.0	36.0
38	33.0163830335601	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	7.0
8	3.0
9	8.0
10	13.0
11	13.0
12	14.0
13	5.0
14	170.0
15	366.0
16	498.0
17	586.0
18	715.0
19	1067.0
20	1344.0
21	2077.0
22	3128.0
23	4650.0
24	7157.0
25	10743.0
26	15815.0
27	22363.0
28	30870.0
29	41603.0
30	55681.0
31	74778.0
32	101121.0
33	148674.0
34	318111.0
35	597896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.23479928412392	16.846876110731255	11.312747838703912	25.605576766440908
2	16.90873236812893	19.658306798205828	37.116578702805874	26.31638213085936
3	19.110534583875566	24.119257762100066	28.011997757194802	28.758209896829573
4	12.430059120624136	14.992809653821304	35.713789486116035	36.863341739438525
5	14.781965950133277	36.55126132616221	33.08472452147551	15.582048202229004
6	34.61171874131626	35.33906876965999	16.63116753920882	13.418044949814934
7	30.604309759725233	30.173945120345792	20.82548036543828	18.396264754490694
8	28.97032649607563	32.017289744947085	19.273327747928747	19.739056011048543
9	27.87889145233416	13.908462078724861	18.31758794388764	39.89505852505334
10	16.21215689461973	26.07715516133447	30.738059957081216	26.972627986964582
11	38.322151893897555	20.937254027437078	21.4988617093187	19.24173236934667
12	25.260798039221132	23.56519395204206	28.130688023298145	23.04331998543866
13	29.654091873920883	18.941605205470886	25.286554502155628	26.117748418452603
14	24.138975580338656	19.661064994038902	24.680198870031376	31.519760555591063
15	25.54574714688339	26.721945511448595	22.570681690579296	25.161625651088716
16	26.252926749623086	25.425377437800055	23.467286408070535	24.854409404506324
17	24.150641860788298	25.59598381016902	25.051783139645977	25.201591189396705
18	25.67194851173687	23.9131552401146	26.33799176876968	24.07690447937885
19	25.650171500905618	24.890940430791378	25.30800339875402	24.150884669548983
20	25.916243279238394	23.546894745873754	25.059417819996483	25.477444154891366
21	27.751450801284168	23.747635261644447	24.04616719594658	24.454746741124804
22	26.117014920395835	23.769324399807694	25.00847591866453	25.105184761131937
23	24.24931512750127	23.64291724072287	25.71341030028784	26.394357331488017
24	24.851155425766006	25.021382620011913	25.035765080913503	25.09169687330858
25	25.180254513745343	24.12836164158934	25.01988931466112	25.671494530004203
26	24.765093722792876	25.270377594078898	25.656705746492296	24.30782293663593
27	25.87758363150033	24.39719317747594	24.643484906381353	25.081738284642373
28	24.613514151926523	24.353957954119135	25.77394433714516	25.25858355680918
29	24.302906210462467	24.834554647164552	25.87471522555803	24.987823916814943
30	24.4371344013561	24.973808895249476	26.077517333370388	24.51153937002404
31	25.110429950798057	24.957099405713873	24.455006134611295	25.477464508876775
32	24.775369070024826	24.722289960614464	24.435843405511196	26.06649756384951
33	24.22507010079508	24.239937861098994	25.264562762791137	26.270429275314793
34	25.376864346899314	24.682226426394298	25.338443185663138	24.60246604104325
35	26.122935630655324	24.36478671744343	25.397483812648474	24.114793839252773
36	24.602861937070735	25.40599693587218	24.71139181579103	25.279749311266052
37	25.90857741545071	25.196791858479383	24.625001215836726	24.269629510233177
38	24.557014438845588	24.79135128225258	25.220424613080205	25.43120966582163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	173.5
2	221.0
3	221.0
4	591.5
5	962.0
6	962.0
7	1232.5
8	1503.0
9	1530.5
10	1558.0
11	1558.0
12	1997.0
13	2436.0
14	3130.5
15	3825.0
16	3825.0
17	5494.5
18	7164.0
19	7164.0
20	8572.5
21	9981.0
22	10023.0
23	10065.0
24	10065.0
25	11650.5
26	13236.0
27	13236.0
28	17141.0
29	21046.0
30	25656.0
31	30266.0
32	30266.0
33	38658.0
34	47050.0
35	47050.0
36	52430.0
37	57810.0
38	66803.0
39	75796.0
40	75796.0
41	80794.0
42	85792.0
43	99154.5
44	112517.0
45	112517.0
46	116587.0
47	120657.0
48	120657.0
49	126391.0
50	132125.0
51	132503.0
52	132881.0
53	132881.0
54	126282.0
55	119683.0
56	119683.0
57	116681.5
58	113680.0
59	102460.0
60	91240.0
61	91240.0
62	88283.0
63	85326.0
64	71632.0
65	57938.0
66	57938.0
67	48801.0
68	39664.0
69	39664.0
70	32137.0
71	24610.0
72	19337.0
73	14064.0
74	14064.0
75	10733.5
76	7403.0
77	7403.0
78	7067.0
79	6731.0
80	5345.0
81	3959.0
82	3959.0
83	3952.0
84	3945.0
85	3945.0
86	2663.0
87	1381.0
88	1190.0
89	999.0
90	999.0
91	726.5
92	454.0
93	398.0
94	342.0
95	342.0
96	309.0
97	276.0
98	276.0
99	520.5
100	765.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12490647644943269
2	0.05682619451370185
3	0.014449692492481642
4	0.0032650747458972946
5	0.0
6	3.4734837722311644E-4
7	0.0
8	7.641664298908562E-4
9	5.557574035569864E-4
10	9.72575456224726E-4
11	0.002639847666895685
12	0.003404014096786541
13	0.01542226794870637
14	0.010073102939470377
15	0.024244916730173528
16	0.01160143579925209
17	0.020979841984276233
18	0.005627043711014487
19	0.008753179106022534
20	0.006530149491794589
21	0.0069469675444623295
22	0.007294315921685446
23	0.01097620872025048
24	0.015561207299595616
25	0.018201054966491305
26	0.01986832717716226
27	0.008822648781467159
28	0.006738558518128459
29	0.012504541580032192
30	0.003959771500343528
31	0.007155376570796198
32	0.008058482351576302
33	0.008405830728799418
34	0.011531966123807465
35	0.0163948434049311
36	0.016811661457598836
37	0.009656284886802638
38	0.006669088842683836
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1439477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.57197945748791
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06210244386472	34.02646734136545
2	14.971136785166038	13.944709496279886
3	5.502783032696273	7.688264950732317
4	2.527116390375278	4.707712504769536
5	1.2501199280926192	2.9110279805262858
6	0.7234090740149517	2.0214355520630805
7	0.44066981120222104	1.4366005776391442
8	0.2838679205150823	1.057623277029458
9	0.19832430378175342	0.831271986147997
>10	0.8343312853451159	6.904707046310299
>50	0.0817565015913326	2.737577151701165
>100	0.10010547591015985	10.444097542806425
>500	0.016484414931499363	5.318149247014303
>1k	0.007792632513072426	5.97035534561468
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4889	0.33963724324876327	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3374	0.234390684950159	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2331	0.1619338134614169	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2312	0.16061388962796905	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2250	0.1563067697504024	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2154	0.14963768090771856	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2126	0.1476925299952691	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2088	0.14505268232837343	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2041	0.14178760758247613	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2018	0.14018980504724982	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2015	0.13998139602091594	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2003	0.13914775991558045	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1894	0.1315755652921165	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1875	0.13025564145866866	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1854	0.12879677827433159	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1841	0.1278936724935515	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1748	0.1214329926772015	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1717	0.11927943273841819	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1705	0.1184457966330827	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1698	0.11795950890497034	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1650	0.11462496448362842	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1644	0.1142081464309607	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1584	0.11003996590428329	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1575	0.10941473882528167	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1547	0.10746958791283223	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1517	0.10538549764949354	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1502	0.10434345251782418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.94696754446233E-5	0.0
5	0.0	0.0	0.0	6.94696754446233E-5	0.0
6	0.0	0.0	0.0	6.94696754446233E-5	0.0
7	0.0	0.0	0.0	6.94696754446233E-5	0.0
8	0.0	0.0	0.0	6.94696754446233E-5	0.0
9	0.0	0.0	0.0	3.4734837722311644E-4	0.0
10	0.0	0.0	0.0	3.4734837722311644E-4	0.0
11	0.0	0.0	0.0	4.1681805266773975E-4	0.0
12	0.0	0.0	0.0	4.1681805266773975E-4	0.0
13	0.0	0.0	0.0	4.1681805266773975E-4	0.0
14	0.0	0.0	0.0	4.1681805266773975E-4	0.0
15	0.0	0.0	0.0	4.1681805266773975E-4	0.0
16	0.0	0.0	0.0	4.1681805266773975E-4	0.0
17	0.0	0.0	0.0	4.1681805266773975E-4	0.0
18	0.0	0.0	0.0	4.86287728112363E-4	0.0
19	0.0	0.0	0.0	6.946967544462329E-4	0.0
20	0.0	0.0	0.0	7.641664298908562E-4	0.0
21	0.0	0.0	0.0	8.336361053354795E-4	0.0
22	0.0	0.0	0.0	9.72575456224726E-4	0.0
23	0.0	0.0	0.0	0.0022230296142279455	0.0
24	0.0	0.0	0.0	0.003126135395008048	0.0
25	0.0	0.0	0.0	0.0036818927985650344	0.0
26	0.0	0.0	0.0	0.0041681805266773975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	0.004489769	19.995571	5
ATCGTTT	390	0.0	18.870237	29
CCGTCTA	45	0.008850221	17.778164	9
CATCGTT	405	0.0	17.775694	28
GCATCGT	420	0.0	17.140848	27
GGCATCG	430	0.0	17.114866	26
TAGACCG	75	3.2407603E-5	17.062887	5
ATACCGT	475	0.0	16.838375	6
AAGACGG	630	0.0	16.758192	5
CGTTTAT	430	0.0	16.742805	31
TGCACCG	115	1.84682E-8	16.691956	5
CGGCATC	480	0.0	16.665293	25
CGCAAGA	600	0.0	16.539438	2
TACCGTC	465	0.0	16.514767	7
CAAGACG	625	0.0	16.380371	4
CTAGCGG	530	0.0	16.300543	29
ACCGTCG	495	0.0	16.160282	8
AACCGCG	260	0.0	15.998679	7
TCTAGCG	550	0.0	15.998124	28
TATCCCG	70	3.7034455E-4	15.996457	5
>>END_MODULE