FastQCFastQC Report
Thu 2 Feb 2017
SRR4062817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439477
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55970.38882177346355656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40920.28426991191939854No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24340.16908919003221307No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA24230.16832502360232224No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23170.16096123800519216No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22770.15818245098740724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22740.15797404196107337No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22260.15463949753973144No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21930.15234699825005887No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21350.14831775707427072No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21000.14588631843370892No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT20870.1449832126529288No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG20490.14234336498603312No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20020.13907829024013582No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19940.13852253283657884No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19540.13574374581879392No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA19460.1351879884152369No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17770.1234476132650956No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.1210856442999784No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17090.1187236753348612No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG16660.1157364792907424No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16550.11497231286085154No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT15750.10941473882528167No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15710.1091368601235032No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT15430.10719170921105374No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT15400.10698330018471987No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG15190.10552443700038278No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15160.1053160279740489No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15120.10503814927227043No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14930.10371822543882257No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT14540.10100890809648226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG707.781946E-720.5728593
CGTCGTA3950.019.03863710
ACCGTCG4050.018.5698368
TACCGTC4250.018.4489827
CCGTCGT4150.018.121119
ATACCGT4100.017.953096
GTACCGT1006.809569E-817.6018356
TTAATAC1006.812297E-817.6012213
GTATTAC1051.18967364E-716.7619061
CTAGCGG5150.016.1547829
ATTACAC1601.8189894E-1116.0011123
GTCGTAG4800.016.00055511
TCTAGCG5050.015.84103428
CGCAAGA4950.015.8394852
ACGAAAA3650.015.78027423
TCAGATA5000.015.681092
AAGACGG5350.015.5530235
CAAGACG5350.015.5524824
TAGCGGC5350.015.55086230
CGACCCG3650.015.3440655
Produced by FastQC (version 0.11.5)