##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1439477
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13938465150885	32.0	32.0	32.0	32.0	32.0
2	31.229988391617233	32.0	32.0	32.0	32.0	32.0
3	31.33180245325212	32.0	32.0	32.0	32.0	32.0
4	31.444358610801007	32.0	32.0	32.0	32.0	32.0
5	31.34237851664181	32.0	32.0	32.0	32.0	32.0
6	34.86144690050622	36.0	36.0	36.0	32.0	36.0
7	34.89777676197674	36.0	36.0	36.0	36.0	36.0
8	34.83170068017759	36.0	36.0	36.0	32.0	36.0
9	34.95325941296735	36.0	36.0	36.0	32.0	36.0
10	34.786365464679186	36.0	36.0	36.0	32.0	36.0
11	34.97386550809773	36.0	36.0	36.0	36.0	36.0
12	34.8559344817597	36.0	36.0	36.0	32.0	36.0
13	34.92429055830694	36.0	36.0	36.0	32.0	36.0
14	34.862241633593314	36.0	36.0	36.0	32.0	36.0
15	34.83081841529945	36.0	36.0	36.0	32.0	36.0
16	34.837454158697916	36.0	36.0	36.0	32.0	36.0
17	34.79734028400593	36.0	36.0	36.0	32.0	36.0
18	34.79995581728642	36.0	36.0	36.0	32.0	36.0
19	34.79464555529543	36.0	36.0	36.0	32.0	36.0
20	34.77399777835978	36.0	36.0	36.0	32.0	36.0
21	34.765336299225346	36.0	36.0	36.0	32.0	36.0
22	34.74258498051723	36.0	36.0	36.0	32.0	36.0
23	34.684979336245036	36.0	36.0	36.0	32.0	36.0
24	34.660468350657915	36.0	36.0	36.0	32.0	36.0
25	34.62449625801593	36.0	36.0	36.0	32.0	36.0
26	34.563708207911624	36.0	36.0	36.0	32.0	36.0
27	34.55002685002956	36.0	36.0	36.0	32.0	36.0
28	34.51074452735264	36.0	36.0	36.0	32.0	36.0
29	34.470759171560225	36.0	36.0	36.0	32.0	36.0
30	34.44414394950388	36.0	36.0	36.0	32.0	36.0
31	34.44168750178016	36.0	36.0	36.0	32.0	36.0
32	34.41341820675148	36.0	36.0	36.0	32.0	36.0
33	34.364082232644215	36.0	36.0	36.0	32.0	36.0
34	34.34740673175049	36.0	36.0	36.0	32.0	36.0
35	34.305652678021254	36.0	36.0	36.0	32.0	36.0
36	34.26348319563286	36.0	36.0	36.0	32.0	36.0
37	34.25325031243987	36.0	36.0	36.0	32.0	36.0
38	33.79576262767658	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	39.0
22	115.0
23	353.0
24	1024.0
25	2555.0
26	5356.0
27	10266.0
28	18140.0
29	28355.0
30	43395.0
31	61796.0
32	88809.0
33	142440.0
34	337620.0
35	699209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.49554521757013	18.127810292409364	12.036805451341994	26.339839038678512
2	15.836179276347782	20.605831566486692	36.4215867440652	27.136402413100324
3	17.996953060034997	24.92502485277639	28.386768249857415	28.6912538373312
4	11.903400624257106	15.86034036114656	35.75806667866181	36.47819233593452
5	14.250453463306464	37.05915412333785	32.7263304658567	15.964061947498987
6	34.65771058633427	35.791461990497886	16.249047391781275	13.301780031386572
7	30.354067570656163	30.360667217331923	20.77124165752558	18.514023554486336
8	28.76142375306978	32.23174370499537	19.113818963690616	19.893013578244243
9	27.699687724697537	13.764219989092943	18.149343318732697	40.38674896747682
10	15.953101955381973	26.332667112666318	30.71881666982757	26.99541426212414
11	38.41625787393506	20.891051208106706	21.431831713330755	19.260859204627483
12	25.01688114286032	23.70577904737557	28.13086150793761	23.146478301826498
13	29.695965542993104	18.95760607165807	25.28720540474826	26.059222980600566
14	24.015475075652528	19.625266416390406	24.623543567242525	31.735714940714537
15	25.476266727429476	26.848223347785343	22.40063578646967	25.27487413831551
16	26.287116779219122	25.380676454017674	23.391064949283663	24.941141817479544
17	24.254850893762107	25.604716157326585	24.880286381790054	25.260146567121254
18	25.599297522641905	23.903820623740426	26.334564567547798	24.16231728606987
19	25.64160455498768	24.88278729010606	25.329338363864096	24.14626979104216
20	25.914411970458715	23.54792747643762	25.124680700004237	25.412979853099426
21	27.559650261137055	23.836276306484177	24.171329249434166	24.432744182944603
22	25.987841417403683	23.773356573255423	25.099949495545953	25.13885251379494
23	24.182705500269194	23.471821323746507	25.747581583563452	26.597891592420847
24	24.737595668426795	24.893277211098198	25.196303935387643	25.172823185087363
25	25.044964309904653	24.131923096962435	25.082269577450113	25.740843015682803
26	24.81034765428531	25.099133295727054	25.694349888431624	24.39616916155601
27	25.852792368339333	24.18593697572104	24.7905315611156	25.170739094824025
28	24.45110272689317	24.185103339615708	26.036261781188585	25.32753215230254
29	24.285000732905075	24.539746032760508	26.052795563944404	25.12245767039001
30	24.421717054180096	24.70411128486249	26.25634171299715	24.617829947960264
31	25.107660629520307	24.624637976153842	24.6315849436983	25.636116450627554
32	24.75975649489363	24.573647234377486	24.62901456570685	26.037581705022035
33	24.281666188483733	24.02150225394362	25.505582930467106	26.19124862710554
34	25.26285588446359	24.409420921626396	25.61291357902905	24.714809614880963
35	26.201321730045006	24.051582623411143	25.49933065967709	24.247764986866756
36	24.678129626246196	25.11300979452954	24.958578706016144	25.25028187320812
37	26.019259786199978	24.948383995287173	24.679397225101358	24.35295899341149
38	24.674395404994595	24.430904023408516	25.332482097652203	25.562218473944686
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	67.0
2	83.0
3	83.0
4	427.5
5	772.0
6	772.0
7	1071.0
8	1370.0
9	1524.0
10	1678.0
11	1678.0
12	2150.0
13	2622.0
14	3261.0
15	3900.0
16	3900.0
17	5881.0
18	7862.0
19	7862.0
20	9020.0
21	10178.0
22	10240.0
23	10302.0
24	10302.0
25	11573.0
26	12844.0
27	12844.0
28	16994.0
29	21144.0
30	25599.0
31	30054.0
32	30054.0
33	38958.0
34	47862.0
35	47862.0
36	52573.5
37	57285.0
38	66458.5
39	75632.0
40	75632.0
41	80336.0
42	85040.0
43	98098.5
44	111157.0
45	111157.0
46	116099.0
47	121041.0
48	121041.0
49	127594.5
50	134148.0
51	133591.0
52	133034.0
53	133034.0
54	126574.0
55	120114.0
56	120114.0
57	117388.0
58	114662.0
59	103231.0
60	91800.0
61	91800.0
62	88931.5
63	86063.0
64	71729.5
65	57396.0
66	57396.0
67	48678.5
68	39961.0
69	39961.0
70	32471.5
71	24982.0
72	19382.5
73	13783.0
74	13783.0
75	10383.0
76	6983.0
77	6983.0
78	6867.5
79	6752.0
80	5306.0
81	3860.0
82	3860.0
83	3567.0
84	3274.0
85	3274.0
86	2130.0
87	986.0
88	783.5
89	581.0
90	581.0
91	358.0
92	135.0
93	90.5
94	46.0
95	46.0
96	28.0
97	10.0
98	10.0
99	20.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008683709430577911
2	6.94696754446233E-5
3	0.0
4	4.1681805266773975E-4
5	0.0
6	5.557574035569864E-4
7	4.1681805266773975E-4
8	0.0029177263686741783
9	0.0029177263686741783
10	9.72575456224726E-4
11	0.00527969533379137
12	6.94696754446233E-5
13	1.389393508892466E-4
14	6.94696754446233E-5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.0840902633386988E-4
22	0.0
23	1.389393508892466E-4
24	0.0
25	1.389393508892466E-4
26	6.94696754446233E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	6.94696754446233E-5
38	6.94696754446233E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1439477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.02825277603758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.70764192317075	31.13133931790914
2	16.139433427619217	14.211821092264989
3	6.004203861982881	7.930638160627302
4	2.7558986710412925	4.853496132550084
5	1.424199744750815	3.135251318272854
6	0.7945377514962316	2.098926537778837
7	0.49937855495558764	1.5390735673961893
8	0.3018934343574601	1.0633472351453088
9	0.22266698605489976	0.8823274512213206
>10	0.9204521833073668	7.068891192715489
>50	0.09243925424879927	2.8905786032691196
>100	0.10901001248737592	10.662823861977841
>500	0.019199705268587365	5.844859505516318
>1k	0.008885814008602416	6.295608743884429
>5k	1.586752501536146E-4	0.39101727947091103
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5597	0.38882177346355656	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4092	0.28426991191939854	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2434	0.16908919003221307	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2423	0.16832502360232224	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2317	0.16096123800519216	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2277	0.15818245098740724	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2274	0.15797404196107337	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2226	0.15463949753973144	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2193	0.15234699825005887	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2135	0.14831775707427072	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2100	0.14588631843370892	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2087	0.1449832126529288	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2049	0.14234336498603312	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2002	0.13907829024013582	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1994	0.13852253283657884	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1954	0.13574374581879392	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1946	0.1351879884152369	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1777	0.1234476132650956	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1743	0.1210856442999784	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1709	0.1187236753348612	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1666	0.1157364792907424	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1655	0.11497231286085154	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1575	0.10941473882528167	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1571	0.1091368601235032	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1543	0.10719170921105374	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1540	0.10698330018471987	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1519	0.10552443700038278	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1516	0.1053160279740489	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1512	0.10503814927227043	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1493	0.10371822543882257	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1454	0.10100890809648226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	6.94696754446233E-5	0.0
4	0.0	0.0	0.0	6.94696754446233E-5	0.0
5	0.0	0.0	0.0	1.389393508892466E-4	0.0
6	0.0	0.0	0.0	1.389393508892466E-4	0.0
7	0.0	0.0	0.0	1.389393508892466E-4	0.0
8	0.0	0.0	0.0	1.389393508892466E-4	0.0
9	0.0	0.0	0.0	1.389393508892466E-4	0.0
10	0.0	0.0	0.0	1.389393508892466E-4	0.0
11	0.0	0.0	0.0	2.0840902633386988E-4	0.0
12	0.0	0.0	0.0	2.0840902633386988E-4	0.0
13	0.0	0.0	0.0	2.0840902633386988E-4	0.0
14	0.0	0.0	0.0	2.778787017784932E-4	0.0
15	0.0	0.0	0.0	2.778787017784932E-4	0.0
16	0.0	0.0	0.0	2.778787017784932E-4	0.0
17	0.0	0.0	0.0	2.778787017784932E-4	0.0
18	0.0	0.0	0.0	3.4734837722311644E-4	0.0
19	0.0	0.0	0.0	4.1681805266773975E-4	0.0
20	0.0	0.0	0.0	4.86287728112363E-4	0.0
21	0.0	0.0	0.0	5.557574035569864E-4	0.0
22	0.0	0.0	0.0	8.336361053354795E-4	0.0
23	0.0	0.0	0.0	0.0018062115615602055	0.0
24	0.0	0.0	0.0	0.002570377991451062	0.0
25	0.0	0.0	0.0	0.003126135395008048	0.0
26	0.0	0.0	0.0	0.0035429534476757877	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGG	70	7.781946E-7	20.572859	3
CGTCGTA	395	0.0	19.038637	10
ACCGTCG	405	0.0	18.569836	8
TACCGTC	425	0.0	18.448982	7
CCGTCGT	415	0.0	18.12111	9
ATACCGT	410	0.0	17.95309	6
GTACCGT	100	6.809569E-8	17.601835	6
TTAATAC	100	6.812297E-8	17.601221	3
GTATTAC	105	1.18967364E-7	16.761906	1
CTAGCGG	515	0.0	16.15478	29
ATTACAC	160	1.8189894E-11	16.001112	3
GTCGTAG	480	0.0	16.000555	11
TCTAGCG	505	0.0	15.841034	28
CGCAAGA	495	0.0	15.839485	2
ACGAAAA	365	0.0	15.780274	23
TCAGATA	500	0.0	15.68109	2
AAGACGG	535	0.0	15.553023	5
CAAGACG	535	0.0	15.552482	4
TAGCGGC	535	0.0	15.550862	30
CGACCCG	365	0.0	15.344065	5
>>END_MODULE