FastQCFastQC Report
Thu 2 Feb 2017
SRR4062816_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062816_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310631
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32860.25071892851611166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22730.17342791373010405No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20490.1563369094733758No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19750.15069077413856377No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19150.1461128265697973No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18540.1414585798748847No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18140.13840661482904035No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17710.13512575240475772No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17700.1350494532786116No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16820.12833513017775408No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16700.12741954066400077No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16680.12726694241170855No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16250.1239860799874259No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14910.11376199708384739No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14460.11032853640727254No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14290.1090314512627887No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13990.10674247747840544No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13910.10613208446923657No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13650.10414830718943775No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13560.10346161505412278No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13540.10330901680183056No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13110.1000281543775479No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG302.2497501E-531.9932885
TATGCCG350.002069217222.8523465
TTAAGCG400.004485294719.99885626
TTAGACG400.00448727619.997334
GTCTAGA1600.018.022351
GCGAACA450.008828565517.7855822
GATATAC1651.8189894E-1216.5053181
TCTAGCG4050.016.19660428
GTTCAAA4750.016.1884981
GATCTAT1101.9915024E-716.0198691
GTCCTAC2800.016.0198671
CCCTATA908.546951E-616.0070252
CGACAAA600.002442461415.99969514
CAAGACG4850.015.8329374
TAGGACG3850.015.7900994
TCTAGAT4000.015.606852
TAACGAA1753.6379788E-1215.54256113
CTAGCGG4250.015.4344129
AACGAAT1153.347959E-715.303472531
GATAACG2000.015.1997111
Produced by FastQC (version 0.11.5)