##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062816_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310631
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19845936804486	32.0	32.0	32.0	32.0	32.0
2	30.737739302671766	32.0	32.0	32.0	32.0	32.0
3	30.798754187868287	32.0	32.0	32.0	32.0	32.0
4	30.876796749046832	32.0	32.0	32.0	32.0	32.0
5	30.752591690567368	32.0	32.0	32.0	32.0	32.0
6	34.4254637651635	36.0	36.0	36.0	32.0	36.0
7	34.34542674482749	36.0	36.0	36.0	32.0	36.0
8	34.319422476654374	36.0	36.0	36.0	32.0	36.0
9	34.497891473648956	36.0	36.0	36.0	32.0	36.0
10	34.13958772530178	36.0	36.0	36.0	32.0	36.0
11	34.48340913651516	36.0	36.0	36.0	32.0	36.0
12	34.26057830159671	36.0	36.0	36.0	32.0	36.0
13	34.376034139281	36.0	36.0	36.0	32.0	36.0
14	34.25983972605562	36.0	36.0	36.0	32.0	36.0
15	34.18528937588078	36.0	36.0	36.0	32.0	36.0
16	34.21346816914906	36.0	36.0	36.0	32.0	36.0
17	34.11957675348744	36.0	36.0	36.0	32.0	36.0
18	34.158602993519914	36.0	36.0	36.0	32.0	36.0
19	34.143683462393305	36.0	36.0	36.0	32.0	36.0
20	34.12490014351866	36.0	36.0	36.0	32.0	36.0
21	34.10615421121582	36.0	36.0	36.0	32.0	36.0
22	34.07703617570468	36.0	36.0	36.0	32.0	36.0
23	34.01617846670802	36.0	36.0	36.0	32.0	36.0
24	33.99248606205713	36.0	36.0	36.0	32.0	36.0
25	33.97238734624772	36.0	36.0	36.0	32.0	36.0
26	33.920262072238486	36.0	36.0	36.0	32.0	36.0
27	33.92068095444103	36.0	36.0	36.0	32.0	36.0
28	33.903692191013334	36.0	36.0	36.0	32.0	36.0
29	33.86331621943934	36.0	36.0	36.0	32.0	36.0
30	33.82826516387908	36.0	36.0	36.0	32.0	36.0
31	33.858041660848855	36.0	36.0	36.0	32.0	36.0
32	33.797004648905755	36.0	36.0	36.0	32.0	36.0
33	33.758850507885136	36.0	36.0	36.0	32.0	36.0
34	33.766924481413916	36.0	36.0	36.0	32.0	36.0
35	33.72493173135688	36.0	36.0	36.0	27.0	36.0
36	33.671016479848255	36.0	36.0	36.0	27.0	36.0
37	33.66676509253939	36.0	36.0	36.0	27.0	36.0
38	33.03951836939611	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	1.0
8	3.0
9	5.0
10	7.0
11	14.0
12	6.0
13	7.0
14	138.0
15	272.0
16	409.0
17	435.0
18	597.0
19	810.0
20	1107.0
21	1683.0
22	2752.0
23	4314.0
24	6514.0
25	9900.0
26	14439.0
27	20465.0
28	28515.0
29	38050.0
30	51104.0
31	68461.0
32	91398.0
33	135668.0
34	287960.0
35	545596.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96021825798473	17.01521587847464	11.220745966864081	25.803819896676554
2	16.804510761610892	19.41212305352789	37.51127919421894	26.27208699064228
3	18.81734952850561	23.84392688670247	28.377949415267594	28.960774169524324
4	12.195635586754157	15.002975736303984	36.242865862963534	36.55852281397833
5	14.517817467099642	36.75653734404376	33.52754107966892	15.198104109187685
6	34.53606556251321	35.763798548328396	16.68422823579859	13.015907653359804
7	30.589921953623865	30.322951311238633	20.83774914525904	18.249377589878463
8	28.59188769955144	32.76332625529613	19.29160623121605	19.353179813936382
9	27.73025186360758	13.726452164226362	18.409924997901772	40.13337097426429
10	15.884125335813579	26.23286788038971	31.546371458459067	26.336635325337642
11	38.168086872657575	20.84231135473002	21.96331778838524	19.02628398422717
12	24.771879117869005	23.74349438691774	28.622790037227254	22.861836457986005
13	29.446014305020647	18.98639321331849	25.478137645951882	26.08945483570898
14	23.65214219655649	19.855187695914	24.75154976177468	31.741120345754826
15	25.279238797632388	27.426454651741373	22.230506705089027	25.063799845537215
16	26.064940297414108	25.48630442214246	23.79076319195175	24.657992088491685
17	24.03933473443865	25.77568273359005	25.303229410391076	24.88175312158023
18	25.24554239078018	24.235293399503117	26.58709811318451	23.93206609653219
19	25.691180858069192	24.76813116965664	25.28486196984923	24.25582600242494
20	25.793509694255174	23.95354692996177	25.16500454000931	25.08793883577375
21	27.415235646238777	23.961481673654852	24.063042474695642	24.56024020541073
22	25.929252225985717	24.315493654157095	24.905249198233097	24.85000492162409
23	24.182951675301982	23.97898528053964	25.614302828669754	26.22376021548863
24	24.83685018604268	25.082719550201	25.131862899213402	24.94856736454292
25	24.96651854700796	24.375108266236882	25.208271807586378	25.45010137916878
26	24.44648453865508	25.529362475646693	25.754942967665524	24.2692100180327
27	25.51441648086171	24.742963447929593	24.812783381177155	24.929836690031546
28	24.35756960711751	24.58869041714674	25.818842183172208	25.234897792563544
29	24.34950152423321	24.730578903391468	25.785447483584445	25.134472088790876
30	24.285726275888013	25.118858047134246	26.067814896570034	24.527600780407706
31	25.126988571982317	24.912193420935164	24.55310781495722	25.4077101921253
32	24.601179198672924	24.878161933415793	24.661611795344097	25.859047072567186
33	24.157136185915338	24.527062292259938	25.524748727997487	25.79105279382724
34	25.28163495165486	24.673228673921553	25.60411393443436	24.441022439989226
35	26.054623708429357	24.43949268173563	25.45908945223669	24.046794157598328
36	24.369091251790415	25.40537570653157	25.026269581998868	25.19926345967915
37	25.779370368702942	25.122528353400536	24.7358095646514	24.362291713245117
38	24.508317493939572	24.93165088577247	25.36185161813619	25.198180002151766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	90.5
2	139.0
3	139.0
4	384.0
5	629.0
6	629.0
7	771.0
8	913.0
9	972.5
10	1032.0
11	1032.0
12	1368.0
13	1704.0
14	2258.0
15	2812.0
16	2812.0
17	4095.0
18	5378.0
19	5378.0
20	6468.0
21	7558.0
22	8259.0
23	8960.0
24	8960.0
25	10859.5
26	12759.0
27	12759.0
28	16707.5
29	20656.0
30	25438.5
31	30221.0
32	30221.0
33	38824.0
34	47427.0
35	47427.0
36	52521.0
37	57615.0
38	66119.0
39	74623.0
40	74623.0
41	78145.5
42	81668.0
43	92300.0
44	102932.0
45	102932.0
46	105220.5
47	107509.0
48	107509.0
49	113665.5
50	119822.0
51	120721.0
52	121620.0
53	121620.0
54	114757.5
55	107895.0
56	107895.0
57	105086.0
58	102277.0
59	91665.5
60	81054.0
61	81054.0
62	78085.0
63	75116.0
64	62423.5
65	49731.0
66	49731.0
67	41950.0
68	34169.0
69	34169.0
70	27559.0
71	20949.0
72	16293.5
73	11638.0
74	11638.0
75	8825.0
76	6012.0
77	6012.0
78	5800.0
79	5588.0
80	4429.0
81	3270.0
82	3270.0
83	3281.5
84	3293.0
85	3293.0
86	2227.0
87	1161.0
88	1006.5
89	852.0
90	852.0
91	631.5
92	411.0
93	359.5
94	308.0
95	308.0
96	273.5
97	239.0
98	239.0
99	444.0
100	649.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11734805601271449
2	0.05318049092383745
3	0.008926997759094665
4	0.0023652729105293558
5	5.340938830227577E-4
6	3.05196504584433E-4
7	0.0
8	3.05196504584433E-4
9	7.629912614610824E-5
10	6.10393009168866E-4
11	0.00175487990136049
12	0.002441572036675464
13	0.0138864409585917
14	0.008164006497633582
15	0.02159265269934863
16	0.010300382029724614
17	0.018082892896627654
18	0.00480684494720482
19	0.006409126596273093
20	0.004654246694912603
21	0.00656172484856531
22	0.006714323100857526
23	0.009232194263679099
24	0.013657543580153378
25	0.015565021733806084
26	0.01785399551818933
27	0.008926997759094665
28	0.004654246694912603
29	0.010376681155870723
30	0.0025941702889676808
31	0.006103930091688659
32	0.006103930091688659
33	0.007553613488464717
34	0.012055261931085104
35	0.01609911561682884
36	0.013733842706299484
37	0.009003296885240774
38	0.006103930091688659
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1310631.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.37345402781922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.88956236915385	38.731928352217096
2	13.58380618214931	13.685264724786094
3	4.458263949040972	6.737344622426973
4	1.8368796124982174	3.701198828592693
5	0.9387373032390449	2.364372019445551
6	0.5202743500427458	1.5724809632239065
7	0.3347320661052314	1.180312724051217
8	0.2375627939081254	0.9573486782120996
9	0.16178441406329988	0.7334675769811773
>10	0.8354142452865865	7.685084338279741
>50	0.08678955058005346	3.0853357704013393
>100	0.09614415985220987	10.404426825139945
>500	0.013973548298378715	4.6954446676074495
>1k	0.006075455781903789	4.4659899086347465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3286	0.25071892851611166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2273	0.17342791373010405	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2049	0.1563369094733758	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1975	0.15069077413856377	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1915	0.1461128265697973	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1854	0.1414585798748847	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1814	0.13840661482904035	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1771	0.13512575240475772	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1770	0.1350494532786116	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1682	0.12833513017775408	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1670	0.12741954066400077	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1668	0.12726694241170855	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1625	0.1239860799874259	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1491	0.11376199708384739	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1446	0.11032853640727254	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1429	0.1090314512627887	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1399	0.10674247747840544	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1391	0.10613208446923657	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1365	0.10414830718943775	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1356	0.10346161505412278	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1354	0.10330901680183056	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1311	0.1000281543775479	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.629912614610824E-5
2	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
3	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
4	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
5	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
6	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
7	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
8	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
9	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
10	0.0	0.0	0.0	1.525982522922165E-4	7.629912614610824E-5
11	0.0	0.0	0.0	2.2889737843832475E-4	7.629912614610824E-5
12	0.0	0.0	0.0	3.05196504584433E-4	1.525982522922165E-4
13	0.0	0.0	0.0	3.05196504584433E-4	1.525982522922165E-4
14	0.0	0.0	0.0	3.05196504584433E-4	1.525982522922165E-4
15	0.0	0.0	0.0	3.05196504584433E-4	1.525982522922165E-4
16	0.0	0.0	0.0	3.05196504584433E-4	1.525982522922165E-4
17	0.0	0.0	0.0	3.8149563073054123E-4	1.525982522922165E-4
18	0.0	0.0	0.0	4.577947568766495E-4	1.525982522922165E-4
19	0.0	0.0	0.0	6.10393009168866E-4	1.525982522922165E-4
20	0.0	0.0	0.0	6.10393009168866E-4	2.2889737843832475E-4
21	0.0	0.0	0.0	7.629912614610825E-4	2.2889737843832475E-4
22	0.0	0.0	0.0	9.918886398994071E-4	2.2889737843832475E-4
23	0.0	0.0	0.0	0.0013733842706299484	2.2889737843832475E-4
24	0.0	0.0	0.0	0.0020600764059449227	2.2889737843832475E-4
25	0.0	0.0	0.0	0.002975665919698222	2.2889737843832475E-4
26	0.0	0.0	0.0	0.003586058928867088	4.577947568766495E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	30	2.2497501E-5	31.993288	5
TATGCCG	35	0.0020692172	22.852346	5
TTAAGCG	40	0.0044852947	19.998856	26
TTAGACG	40	0.004487276	19.99733	4
GTCTAGA	160	0.0	18.02235	1
GCGAACA	45	0.0088285655	17.785582	2
GATATAC	165	1.8189894E-12	16.505318	1
TCTAGCG	405	0.0	16.196604	28
GTTCAAA	475	0.0	16.188498	1
GATCTAT	110	1.9915024E-7	16.019869	1
GTCCTAC	280	0.0	16.019867	1
CCCTATA	90	8.546951E-6	16.007025	2
CGACAAA	60	0.0024424614	15.999695	14
CAAGACG	485	0.0	15.832937	4
TAGGACG	385	0.0	15.790099	4
TCTAGAT	400	0.0	15.60685	2
TAACGAA	175	3.6379788E-12	15.542561	13
CTAGCGG	425	0.0	15.43441	29
AACGAAT	115	3.347959E-7	15.3034725	31
GATAACG	200	0.0	15.19971	11
>>END_MODULE