FastQCFastQC Report
Thu 2 Feb 2017
SRR4062816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310631
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35660.272082683837022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27270.20806771700043722No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22710.17327531547781183No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19860.151530064526171No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19740.15061447501241768No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19370.14779140734501167No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19170.1462654248220895No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17940.1368806323061182No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17780.13565984628778047No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG17300.13199748823276727No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17090.130395206583699No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17050.13009001007911455No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16440.12543576338420195No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16370.1249016695011792No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15590.11895033766178276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15080.11505908222833124No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14190.1082684600013276No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13900.10605578534309047No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13880.10590318709079824No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC13880.10590318709079824No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13840.10559799058621382No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13770.10506389670319105No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA13640.10407200806329166No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13600.1037668115587072No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13510.10308011942339224No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13410.10231712816193117No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13370.10201193165734672No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT13230.10094374389130123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3900.021.33307829
TAGCGGC4100.020.2924430
CGGCATC3000.020.26642625
TCTAGCG4250.019.95270328
GTCCTAA2300.019.4802571
GCATCGT3350.018.62664227
CATCGTT3450.018.55050328
CAACGGA1055.980837E-918.28549814
GGCATCG3450.017.62297826
CAAGACG4650.017.5488474
GGACCGT550.001364544317.4550046
AGCGGCG4950.017.1311131
ATCGTTT3700.016.86466429
AAGACGG4650.016.8606575
GATATAA957.686176E-716.8438321
CGCAAGA4700.016.000422
CTAATGT805.6205623E-516.000424
TCGACGC703.697226E-415.9998114
AACGGAC1203.1113814E-815.99980915
CGTTTAT4000.015.99980931
Produced by FastQC (version 0.11.5)