##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310631
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175053085117018	32.0	32.0	32.0	32.0	32.0
2	31.24952789915697	32.0	32.0	32.0	32.0	32.0
3	31.356312341154755	32.0	32.0	32.0	32.0	32.0
4	31.467213883999385	32.0	32.0	32.0	32.0	32.0
5	31.375921979565568	32.0	32.0	32.0	32.0	32.0
6	34.90949702853053	36.0	36.0	36.0	36.0	36.0
7	34.93498704059343	36.0	36.0	36.0	36.0	36.0
8	34.861026482663696	36.0	36.0	36.0	32.0	36.0
9	34.98078864302767	36.0	36.0	36.0	36.0	36.0
10	34.8239527372693	36.0	36.0	36.0	32.0	36.0
11	35.00656859176992	36.0	36.0	36.0	36.0	36.0
12	34.89309042743533	36.0	36.0	36.0	32.0	36.0
13	34.96594846299225	36.0	36.0	36.0	36.0	36.0
14	34.89943164780934	36.0	36.0	36.0	32.0	36.0
15	34.86586461025262	36.0	36.0	36.0	32.0	36.0
16	34.87082023849581	36.0	36.0	36.0	32.0	36.0
17	34.833715210459694	36.0	36.0	36.0	32.0	36.0
18	34.84032195179269	36.0	36.0	36.0	32.0	36.0
19	34.83290262476624	36.0	36.0	36.0	32.0	36.0
20	34.82696121181324	36.0	36.0	36.0	32.0	36.0
21	34.816277045179	36.0	36.0	36.0	32.0	36.0
22	34.794133512788875	36.0	36.0	36.0	32.0	36.0
23	34.73247466296768	36.0	36.0	36.0	32.0	36.0
24	34.712177569430295	36.0	36.0	36.0	32.0	36.0
25	34.681313809912936	36.0	36.0	36.0	32.0	36.0
26	34.61546690105758	36.0	36.0	36.0	32.0	36.0
27	34.599447899523206	36.0	36.0	36.0	32.0	36.0
28	34.5756410461831	36.0	36.0	36.0	32.0	36.0
29	34.530955699964366	36.0	36.0	36.0	32.0	36.0
30	34.50377489926608	36.0	36.0	36.0	32.0	36.0
31	34.500755742844476	36.0	36.0	36.0	32.0	36.0
32	34.47911959964323	36.0	36.0	36.0	32.0	36.0
33	34.4462178904665	36.0	36.0	36.0	32.0	36.0
34	34.4306032743007	36.0	36.0	36.0	32.0	36.0
35	34.39177388601368	36.0	36.0	36.0	32.0	36.0
36	34.34647967276831	36.0	36.0	36.0	32.0	36.0
37	34.34394196383269	36.0	36.0	36.0	32.0	36.0
38	33.90386615302095	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	27.0
22	87.0
23	291.0
24	826.0
25	2124.0
26	4624.0
27	8881.0
28	15487.0
29	24597.0
30	37223.0
31	54014.0
32	77851.0
33	125655.0
34	302822.0
35	656113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.214731828437955	18.264845409046618	11.860608322021056	26.659814440494365
2	15.798878555443904	20.399258067297353	36.741310101775404	27.060553275483336
3	17.774339230492796	24.41274470083494	28.80948184500443	29.003434223667835
4	11.6171026408714	15.835767281028637	36.33639751325319	36.21073256484678
5	13.932307363634283	37.23339157504406	33.28467988677201	15.549621174549644
6	34.6178819482859	36.04048132614188	16.33246326763714	13.009173457935082
7	30.27161107991308	30.52622262372732	20.892033107893646	18.31013318846595
8	28.365405157942924	33.01426827407295	19.090721806806044	19.52960476117809
9	27.350270833667157	13.634008681632526	18.21990671327188	40.79581377142844
10	15.583869594344044	26.48422347320119	31.513835457776405	26.418071474678356
11	38.21469230041417	20.770904740249236	21.885997173735888	19.1284057856007
12	24.51128427648099	23.79971754020398	28.68172457652013	23.007273606794907
13	29.433860052036042	19.0201658744268	25.47667915429984	26.06929491923731
14	23.47251056933645	19.763838944752564	24.78050648885918	31.983143997051798
15	25.23051873486893	27.457156133190807	22.082798285711235	25.229526846229028
16	25.967720891692625	25.613921843753122	23.686911113807014	24.731446150747235
17	23.96746300064625	25.925603774059976	25.134992228934	24.971940996359766
18	25.3408472712762	24.227566721678336	26.524170418676196	23.907415588369265
19	25.675953033309913	24.799733868648	25.237080459717493	24.287232638324593
20	25.781322126517686	23.87384397286498	25.167114161041514	25.17771973957582
21	27.307783279796737	23.97205923868674	24.09055187200049	24.629605609516034
22	25.72333479064664	24.30317915568913	25.046866738235245	24.92661931542898
23	24.036245192193977	23.877313869905215	25.774723434320467	26.31171750358034
24	24.764788868873087	25.012379533217207	25.218844968568575	25.003986629341135
25	24.919065701940514	24.317065596647723	25.334667042058367	25.429201659353396
26	24.53835178501942	25.42265933177174	25.842915239236092	24.196073643972746
27	25.57022195432731	24.525838718784097	24.860486941394598	25.043452385493996
28	24.25312692893728	24.487594143584275	25.991678817302503	25.267600110175938
29	24.34651705934012	24.665676304009292	25.82862758472827	25.15917905192232
30	24.239774581861713	24.967820843547877	26.152593674344647	24.63981090024576
31	25.069680176952936	24.769519414694145	24.670025354199616	25.490775054153303
32	24.62554296365644	24.731980244630257	24.732056543756403	25.9104202479569
33	24.104267333826225	24.45432772458457	25.574856691166314	25.866548250422888
34	25.143766628440805	24.54001164324665	25.74843720314871	24.567784525163834
35	26.06546007228579	24.33140983236319	25.479177586979095	24.12395250837192
36	24.412134307825774	25.229984640985908	25.141325056404128	25.21655599478419
37	25.770106154974208	24.929518682222533	24.83269509114312	24.46768007166014
38	24.5311609446137	24.62523776715185	25.46887720494937	25.374724083285074
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	47.0
2	69.0
3	69.0
4	302.5
5	536.0
6	536.0
7	710.5
8	885.0
9	991.0
10	1097.0
11	1097.0
12	1363.5
13	1630.0
14	2241.5
15	2853.0
16	2853.0
17	4307.5
18	5762.0
19	5762.0
20	6633.5
21	7505.0
22	8375.0
23	9245.0
24	9245.0
25	10898.5
26	12552.0
27	12552.0
28	16509.5
29	20467.0
30	25263.5
31	30060.0
32	30060.0
33	38900.0
34	47740.0
35	47740.0
36	52668.5
37	57597.0
38	66354.5
39	75112.0
40	75112.0
41	78269.0
42	81426.0
43	92035.5
44	102645.0
45	102645.0
46	105273.0
47	107901.0
48	107901.0
49	114526.5
50	121152.0
51	121016.5
52	120881.0
53	120881.0
54	114660.0
55	108439.0
56	108439.0
57	105882.5
58	103326.0
59	92207.5
60	81089.0
61	81089.0
62	78393.5
63	75698.0
64	62755.5
65	49813.0
66	49813.0
67	41873.5
68	33934.0
69	33934.0
70	27370.5
71	20807.0
72	16266.0
73	11725.0
74	11725.0
75	8765.5
76	5806.0
77	5806.0
78	5690.0
79	5574.0
80	4318.5
81	3063.0
82	3063.0
83	2914.5
84	2766.0
85	2766.0
86	1751.0
87	736.0
88	633.0
89	530.0
90	530.0
91	322.0
92	114.0
93	77.5
94	41.0
95	41.0
96	30.5
97	20.0
98	20.0
99	15.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008240305623779691
2	0.0
3	0.0
4	2.2889737843832475E-4
5	7.629912614610824E-5
6	1.525982522922165E-4
7	5.340938830227577E-4
8	0.0023652729105293558
9	0.003662358055013196
10	9.918886398994073E-4
11	0.005112041451789253
12	1.525982522922165E-4
13	7.629912614610824E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	7.629912614610824E-5
22	0.0
23	1.525982522922165E-4
24	0.0
25	0.0
26	7.629912614610824E-5
27	7.629912614610824E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1310631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.86038731805844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69142918151516	35.747607299666484
2	14.790168808467936	14.157264153454843
3	4.8592687686922185	6.976994560564635
4	2.0721854008748366	3.9670238352278355
5	1.062361342263428	2.5422512656230056
6	0.5918624815505676	1.699606056362243
7	0.3668000489912261	1.2288634689102023
8	0.24908469155005106	0.953703185006765
9	0.1852198217617865	0.7978223167650766
>10	0.9118363321724937	7.980203920056763
>50	0.09239420077385072	3.1181588201127366
>100	0.10562398855495732	10.996753334194835
>500	0.01456330064463147	4.715125351173024
>1k	0.007201632186905671	5.118622432881591
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3566	0.272082683837022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2727	0.20806771700043722	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2271	0.17327531547781183	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1986	0.151530064526171	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1974	0.15061447501241768	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1937	0.14779140734501167	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1917	0.1462654248220895	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1794	0.1368806323061182	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1778	0.13565984628778047	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1730	0.13199748823276727	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1709	0.130395206583699	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1705	0.13009001007911455	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1644	0.12543576338420195	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1637	0.1249016695011792	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1559	0.11895033766178276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1508	0.11505908222833124	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1419	0.1082684600013276	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1390	0.10605578534309047	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1388	0.10590318709079824	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1388	0.10590318709079824	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1384	0.10559799058621382	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1377	0.10506389670319105	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1364	0.10407200806329166	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1360	0.1037668115587072	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1351	0.10308011942339224	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1341	0.10231712816193117	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1337	0.10201193165734672	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1323	0.10094374389130123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.629912614610824E-5
7	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
8	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
9	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
10	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
11	0.0	0.0	0.0	7.629912614610824E-5	7.629912614610824E-5
12	0.0	0.0	0.0	7.629912614610824E-5	2.2889737843832475E-4
13	0.0	0.0	0.0	7.629912614610824E-5	3.05196504584433E-4
14	0.0	0.0	0.0	7.629912614610824E-5	3.05196504584433E-4
15	0.0	0.0	0.0	7.629912614610824E-5	3.05196504584433E-4
16	0.0	0.0	0.0	7.629912614610824E-5	3.05196504584433E-4
17	0.0	0.0	0.0	2.2889737843832475E-4	3.05196504584433E-4
18	0.0	0.0	0.0	4.577947568766495E-4	3.05196504584433E-4
19	0.0	0.0	0.0	5.340938830227577E-4	3.05196504584433E-4
20	0.0	0.0	0.0	5.340938830227577E-4	3.8149563073054123E-4
21	0.0	0.0	0.0	6.10393009168866E-4	3.8149563073054123E-4
22	0.0	0.0	0.0	8.392903876071907E-4	3.8149563073054123E-4
23	0.0	0.0	0.0	0.001220786018337732	3.8149563073054123E-4
24	0.0	0.0	0.0	0.001831179027506598	3.8149563073054123E-4
25	0.0	0.0	0.0	0.002746768541259897	3.8149563073054123E-4
26	0.0	0.0	0.0	0.0032045632981365464	4.577947568766495E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	390	0.0	21.333078	29
TAGCGGC	410	0.0	20.29244	30
CGGCATC	300	0.0	20.266426	25
TCTAGCG	425	0.0	19.952703	28
GTCCTAA	230	0.0	19.480257	1
GCATCGT	335	0.0	18.626642	27
CATCGTT	345	0.0	18.550503	28
CAACGGA	105	5.980837E-9	18.285498	14
GGCATCG	345	0.0	17.622978	26
CAAGACG	465	0.0	17.548847	4
GGACCGT	55	0.0013645443	17.455004	6
AGCGGCG	495	0.0	17.13111	31
ATCGTTT	370	0.0	16.864664	29
AAGACGG	465	0.0	16.860657	5
GATATAA	95	7.686176E-7	16.843832	1
CGCAAGA	470	0.0	16.00042	2
CTAATGT	80	5.6205623E-5	16.00042	4
TCGACGC	70	3.697226E-4	15.99981	14
AACGGAC	120	3.1113814E-8	15.999809	15
CGTTTAT	400	0.0	15.999809	31
>>END_MODULE