FastQCFastQC Report
Thu 2 Feb 2017
SRR4062815_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062815_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104872
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44550.21165182490906811No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32290.1534060028353268No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA30680.1457570816657735No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29190.13867826642190118No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28540.13559019265779582No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG26730.12699109494544086No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26710.1268960772911607No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26580.12627846253833963No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26120.12409305648989583No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG26060.12380800352705533No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25300.12019733266440905No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25170.11957971791158796No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG22820.10841514353366856No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22440.10660980810234541No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT22270.10580215804096402No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA21950.10428187557248138No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT21890.10399682260964088No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21450.10190643421547722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG801.2493183E-720.0263041
CATCGTT6100.016.26047128
TAGCGTA4350.016.181717
ATCGTTT6150.016.12865629
TCTAGCG6250.016.12619228
GTATAGG2250.015.6650181
CTAGCGG6450.015.62652629
TAGGACG3200.015.4975264
ATAGCGT4650.015.4810326
CGGCATC6500.015.26055325
GCATCGT6550.015.14333927
CGCTTCG4900.015.01908132
AGCGTAT4800.014.9979628
GTCCTAC4600.014.97619151
GTCCTAT8200.014.848771
GTATCAA47400.014.7366561
TAGCGGC6850.014.71402930
GTATTAG6100.014.7078431
AATCGTA1103.5479734E-614.54451526
CGTTTAT6600.014.5441731
Produced by FastQC (version 0.11.5)