##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062815_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2104872
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268188279382308	32.0	32.0	32.0	32.0	32.0
2	30.91519626846668	32.0	32.0	32.0	32.0	32.0
3	30.958295801359892	32.0	32.0	32.0	32.0	32.0
4	31.022659810192735	32.0	32.0	32.0	32.0	32.0
5	30.952439388238336	32.0	32.0	32.0	32.0	32.0
6	34.61059104781668	36.0	36.0	36.0	32.0	36.0
7	34.54332662508694	36.0	36.0	36.0	32.0	36.0
8	34.52340474860229	36.0	36.0	36.0	32.0	36.0
9	34.65729745086637	36.0	36.0	36.0	32.0	36.0
10	34.39353366855562	36.0	36.0	36.0	32.0	36.0
11	34.63616124876002	36.0	36.0	36.0	32.0	36.0
12	34.45743399123557	36.0	36.0	36.0	32.0	36.0
13	34.54505072042385	36.0	36.0	36.0	32.0	36.0
14	34.452048865679245	36.0	36.0	36.0	32.0	36.0
15	34.40322831982182	36.0	36.0	36.0	32.0	36.0
16	34.41622055877982	36.0	36.0	36.0	32.0	36.0
17	34.3367957766553	36.0	36.0	36.0	32.0	36.0
18	34.36819341033564	36.0	36.0	36.0	32.0	36.0
19	34.3481518116066	36.0	36.0	36.0	32.0	36.0
20	34.33109281704541	36.0	36.0	36.0	32.0	36.0
21	34.320789577703536	36.0	36.0	36.0	32.0	36.0
22	34.29385539833301	36.0	36.0	36.0	32.0	36.0
23	34.238409746530905	36.0	36.0	36.0	32.0	36.0
24	34.21557747929565	36.0	36.0	36.0	32.0	36.0
25	34.20591085823746	36.0	36.0	36.0	32.0	36.0
26	34.15903769920451	36.0	36.0	36.0	32.0	36.0
27	34.16019501423364	36.0	36.0	36.0	32.0	36.0
28	34.146223143260016	36.0	36.0	36.0	32.0	36.0
29	34.11094498857888	36.0	36.0	36.0	32.0	36.0
30	34.0765728272313	36.0	36.0	36.0	32.0	36.0
31	34.109254624509234	36.0	36.0	36.0	32.0	36.0
32	34.05557012492921	36.0	36.0	36.0	32.0	36.0
33	34.0260044316234	36.0	36.0	36.0	32.0	36.0
34	34.02774610522635	36.0	36.0	36.0	32.0	36.0
35	33.990746230649656	36.0	36.0	36.0	32.0	36.0
36	33.94873560007449	36.0	36.0	36.0	32.0	36.0
37	33.94790847139399	36.0	36.0	36.0	32.0	36.0
38	33.35569953897434	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	4.0
9	6.0
10	14.0
11	21.0
12	10.0
13	12.0
14	216.0
15	522.0
16	633.0
17	790.0
18	985.0
19	1281.0
20	1696.0
21	2469.0
22	3760.0
23	5938.0
24	8568.0
25	13144.0
26	19239.0
27	27563.0
28	39118.0
29	52574.0
30	71164.0
31	96051.0
32	130399.0
33	197880.0
34	445652.0
35	985156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18461790930474	17.382742357829343	11.471259064412326	25.961380668453586
2	16.77349371016565	19.781700156675438	37.36314666646385	26.08165946669506
3	18.422844446851872	24.08691631992603	28.78662396019384	28.70361527302826
4	12.444400734703729	15.494342914318457	36.3158104783651	35.74544587261271
5	14.748865367740574	36.4234889900407	33.50981320910063	15.317832433118102
6	33.99393119070571	35.75993854226003	16.90752976087257	13.33860050616169
7	30.065452942246818	30.615937983847942	21.184148577276233	18.13446049662901
8	28.241471412377628	32.570306271860105	19.663426865989315	19.524795449772956
9	27.8343266670309	13.940181436814235	18.634306713257146	39.59118518289772
10	16.14237926852038	26.090275966490832	31.467078286078344	26.300266478910444
11	37.700841540247175	20.9732277793324	22.32302735944121	19.002903320979215
12	24.87342129980896	23.694791518692597	28.606002327043157	22.825784854455282
13	29.478015646938914	19.125662547842428	25.31810611694103	26.078215688277623
14	23.92484128122942	19.72631286864519	24.84242155287347	31.506424297251918
15	25.246586096516904	26.98285825077364	22.22876955010987	25.541786102599584
16	26.022651033816903	25.443736375264418	23.632727493884907	24.900885097033775
17	24.187970950237812	25.681278508868843	25.06865413191192	25.06209640898142
18	25.307008687513218	24.26826052562187	26.18811304932426	24.23661773754065
19	25.882038239197147	24.759217351269708	25.162267858729443	24.196476550803702
20	25.97175344173565	23.848747976008575	24.774985366446973	25.404513215808798
21	27.527275508890227	23.95503074745561	23.961112296274052	24.55658144738011
22	26.202847512773708	24.038087835211815	24.613177847441506	25.145886804572974
23	24.489385751977387	23.837407366757084	25.305807891114572	26.367398990150953
24	25.033071108585375	24.924924782234353	24.89646272182826	25.14554138735201
25	25.203088233617045	24.1919711247876	25.053646845052324	25.551293796543035
26	24.86800570411134	25.107403512656767	25.49406091006331	24.53052987316858
27	25.654298768088125	24.644509230786774	24.38409157427011	25.317100426854992
28	24.615463558003782	24.461667985909912	25.498802228491662	25.42406622759464
29	24.67994404722536	24.641124909247793	25.433519969286795	25.24541107424006
30	24.638719501331476	24.897935913303133	25.749891320098538	24.713453265266853
31	25.45969742782292	24.605583360335697	24.252525473285715	25.682193738555668
32	24.877133300391503	24.68599148580334	24.318056558592115	26.118818655213044
33	24.545548276007885	24.294536126411675	25.11912742988361	26.04078816769683
34	25.421552789128576	24.38002470778295	25.28827330609142	24.910149196997054
35	26.337877186713598	24.183094736682047	25.075028700022045	24.403999376582302
36	24.817216502046293	25.006331552895904	24.65670629054629	25.51974565451151
37	25.890509044898792	24.78563117110688	24.573768676945093	24.750091107049236
38	24.797564358213805	24.65289111307176	25.140599113525973	25.408945415188455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	374.0
1	353.5
2	333.0
3	333.0
4	710.5
5	1088.0
6	1088.0
7	1297.5
8	1507.0
9	1449.5
10	1392.0
11	1392.0
12	1835.0
13	2278.0
14	3067.0
15	3856.0
16	3856.0
17	5800.5
18	7745.0
19	7745.0
20	9564.5
21	11384.0
22	12839.5
23	14295.0
24	14295.0
25	17083.0
26	19871.0
27	19871.0
28	25722.5
29	31574.0
30	38793.5
31	46013.0
32	46013.0
33	59382.5
34	72752.0
35	72752.0
36	80933.0
37	89114.0
38	102217.5
39	115321.0
40	115321.0
41	121888.5
42	128456.0
43	146291.0
44	164126.0
45	164126.0
46	168123.5
47	172121.0
48	172121.0
49	183730.5
50	195340.0
51	196838.5
52	198337.0
53	198337.0
54	189700.0
55	181063.0
56	181063.0
57	175541.5
58	170020.0
59	152596.5
60	135173.0
61	135173.0
62	129170.5
63	123168.0
64	101366.5
65	79565.0
66	79565.0
67	66522.0
68	53479.0
69	53479.0
70	42909.0
71	32339.0
72	25452.0
73	18565.0
74	18565.0
75	13992.0
76	9419.0
77	9419.0
78	9216.0
79	9013.0
80	7018.0
81	5023.0
82	5023.0
83	5213.5
84	5404.0
85	5404.0
86	3612.5
87	1821.0
88	1570.0
89	1319.0
90	1319.0
91	905.0
92	491.0
93	466.5
94	442.0
95	442.0
96	398.5
97	355.0
98	355.0
99	645.5
100	936.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12148007099719127
2	0.05511023948249585
3	0.012969909809242557
4	0.003705688516926445
5	2.3754413570041316E-4
6	5.225970985409089E-4
7	4.750882714008263E-5
8	4.750882714008263E-4
9	3.325617899805784E-4
10	7.126324071012394E-4
11	0.0019478619127433875
12	0.0026129854927045442
13	0.014585209932005367
14	0.009786818390857021
15	0.025464731347084286
16	0.011592153822180162
17	0.022946763508659908
18	0.006033621046790494
19	0.008219027095234295
20	0.005701059256809915
21	0.006888779935311981
22	0.007506394688133055
23	0.009549274255156608
24	0.01501278937626611
25	0.01748324838755041
26	0.020191251534535118
27	0.008361553576654543
28	0.006176147528210742
29	0.01140211851361983
30	0.0031830914183855357
31	0.00560604160252975
32	0.0072213417252925596
33	0.009169203638035948
34	0.012922400982102475
35	0.016343036536188425
36	0.015820439437647514
37	0.00973930956371694
38	0.006128638701070659
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2104872.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.203293980935406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63840352952315	40.26545294113602
2	12.880291155545311	13.190266691948576
3	3.8198930401801126	5.867733189362142
4	1.5694520413382356	3.214444570464835
5	0.825844131229934	2.1142969916898258
6	0.4882960347188367	1.5001419249260182
7	0.32046673401711695	1.148626667509207
8	0.22249519920481017	0.9113989675384545
9	0.1704840064830975	0.7856408432701565
>10	0.8909456438097726	8.139672930412054
>50	0.07230433030715194	2.606123231014748
>100	0.07943035680247602	8.884532366775897
>500	0.01396596363088583	4.886491826825775
>1k	0.007727833209090158	6.485176857126321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4455	0.21165182490906811	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3229	0.1534060028353268	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3068	0.1457570816657735	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2919	0.13867826642190118	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2854	0.13559019265779582	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2673	0.12699109494544086	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2671	0.1268960772911607	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2658	0.12627846253833963	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2612	0.12409305648989583	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2606	0.12380800352705533	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2530	0.12019733266440905	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2517	0.11957971791158796	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2282	0.10841514353366856	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2244	0.10660980810234541	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2227	0.10580215804096402	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2195	0.10428187557248138	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2189	0.10399682260964088	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2145	0.10190643421547722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	4.750882714008263E-5
5	0.0	0.0	0.0	0.0	4.750882714008263E-5
6	0.0	0.0	0.0	0.0	9.501765428016526E-5
7	0.0	0.0	0.0	0.0	9.501765428016526E-5
8	0.0	0.0	0.0	0.0	9.501765428016526E-5
9	0.0	0.0	0.0	4.750882714008263E-5	1.4252648142024787E-4
10	0.0	0.0	0.0	9.501765428016526E-5	1.4252648142024787E-4
11	0.0	0.0	0.0	9.501765428016526E-5	1.4252648142024787E-4
12	0.0	0.0	0.0	9.501765428016526E-5	2.3754413570041313E-4
13	0.0	0.0	0.0	9.501765428016526E-5	3.325617899805784E-4
14	0.0	0.0	0.0	9.501765428016526E-5	3.8007061712066104E-4
15	0.0	0.0	0.0	1.4252648142024787E-4	4.275794442607436E-4
16	0.0	0.0	0.0	1.4252648142024787E-4	4.275794442607436E-4
17	0.0	0.0	0.0	1.4252648142024787E-4	4.7508827140082627E-4
18	0.0	0.0	0.0	1.9003530856033052E-4	4.7508827140082627E-4
19	0.0	0.0	0.0	1.9003530856033052E-4	4.7508827140082627E-4
20	0.0	0.0	0.0	1.9003530856033052E-4	4.7508827140082627E-4
21	0.0	0.0	0.0	3.325617899805784E-4	5.701059256809915E-4
22	0.0	0.0	0.0	4.7508827140082627E-4	5.701059256809915E-4
23	0.0	0.0	0.0	9.026677156615699E-4	5.701059256809915E-4
24	0.0	0.0	0.0	0.0013302471599223136	5.701059256809915E-4
25	0.0	0.0	0.0	0.0019478619127433878	5.701059256809915E-4
26	0.0	0.0	0.0	0.0023754413570041314	6.651235799611568E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACG	80	1.2493183E-7	20.026304	1
CATCGTT	610	0.0	16.260471	28
TAGCGTA	435	0.0	16.18171	7
ATCGTTT	615	0.0	16.128656	29
TCTAGCG	625	0.0	16.126192	28
GTATAGG	225	0.0	15.665018	1
CTAGCGG	645	0.0	15.626526	29
TAGGACG	320	0.0	15.497526	4
ATAGCGT	465	0.0	15.481032	6
CGGCATC	650	0.0	15.260553	25
GCATCGT	655	0.0	15.143339	27
CGCTTCG	490	0.0	15.019081	32
AGCGTAT	480	0.0	14.997962	8
GTCCTAC	460	0.0	14.9761915	1
GTCCTAT	820	0.0	14.84877	1
GTATCAA	4740	0.0	14.736656	1
TAGCGGC	685	0.0	14.714029	30
GTATTAG	610	0.0	14.707843	1
AATCGTA	110	3.5479734E-6	14.544515	26
CGTTTAT	660	0.0	14.54417	31
>>END_MODULE