FastQCFastQC Report
Thu 2 Feb 2017
SRR4062815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104872
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT49150.2335058853935061No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36200.1719819542470991No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG32830.15597147950089127No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA31450.14941526135555985No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC29530.140293566544664No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28960.1375855633976793No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28750.13658787802773756No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA28130.1336423307450524No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG27270.1295565716110053No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25490.12110000038007061No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC25450.12090996507151028No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA25210.11976975322014831No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25110.11929466494874748No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24300.11544644995040078No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT23130.10988791717501112No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC22490.10684735223804583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22160.10527956094242309No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21810.1036167519925202No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT21090.10019611643843425No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTA453.5383314E-421.33314322
ACGATTA400.004484928719.9998232
TCTAGCG5950.018.82336228
CTAGCGG6100.018.098229
TGCGTAT550.001365238517.4543923
TAGCGGC6700.016.47746530
GTATTAG4600.016.3496231
CTAGCGC600.002442956715.9998589
TAGGACG2850.015.7195324
CTATTAG1255.1364623E-815.3616891
TACTATA2200.015.2729542
CATCGTT6250.015.10386628
TCTTATA2150.014.8839422
CGGCATC6350.014.8660125
ACCGTCG5600.014.8573648
TATACTG2050.014.8294895
AACCGCG4450.014.7417927
ATCGTTT6300.014.73002729
GTATCAA41100.014.4833431
TCGTTTA6400.014.24987230
Produced by FastQC (version 0.11.5)