##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2104872
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230974615083483	32.0	32.0	32.0	32.0	32.0
2	31.31886071932165	32.0	32.0	32.0	32.0	32.0
3	31.399321668966095	32.0	32.0	32.0	32.0	32.0
4	31.495438202418008	32.0	32.0	32.0	32.0	32.0
5	31.421197583511017	32.0	32.0	32.0	32.0	32.0
6	34.951948622053976	36.0	36.0	36.0	36.0	36.0
7	34.98221649582492	36.0	36.0	36.0	36.0	36.0
8	34.91688948306595	36.0	36.0	36.0	36.0	36.0
9	35.02707813111676	36.0	36.0	36.0	36.0	36.0
10	34.87683004002144	36.0	36.0	36.0	32.0	36.0
11	35.045729146475416	36.0	36.0	36.0	36.0	36.0
12	34.94480994568791	36.0	36.0	36.0	36.0	36.0
13	34.99967123891619	36.0	36.0	36.0	36.0	36.0
14	34.94661575620751	36.0	36.0	36.0	32.0	36.0
15	34.92012340892938	36.0	36.0	36.0	32.0	36.0
16	34.93177399860894	36.0	36.0	36.0	36.0	36.0
17	34.89087554967713	36.0	36.0	36.0	32.0	36.0
18	34.89233407067033	36.0	36.0	36.0	32.0	36.0
19	34.88679834213197	36.0	36.0	36.0	32.0	36.0
20	34.877014849359014	36.0	36.0	36.0	32.0	36.0
21	34.86572437658917	36.0	36.0	36.0	32.0	36.0
22	34.84852000501693	36.0	36.0	36.0	32.0	36.0
23	34.788857944806146	36.0	36.0	36.0	32.0	36.0
24	34.75858056926977	36.0	36.0	36.0	32.0	36.0
25	34.74640215652068	36.0	36.0	36.0	32.0	36.0
26	34.68842808493818	36.0	36.0	36.0	32.0	36.0
27	34.66725197541703	36.0	36.0	36.0	32.0	36.0
28	34.646185611286576	36.0	36.0	36.0	32.0	36.0
29	34.60008922157737	36.0	36.0	36.0	32.0	36.0
30	34.57963904693492	36.0	36.0	36.0	32.0	36.0
31	34.578947793500035	36.0	36.0	36.0	32.0	36.0
32	34.55593689307474	36.0	36.0	36.0	32.0	36.0
33	34.51525603457122	36.0	36.0	36.0	32.0	36.0
34	34.51477952103501	36.0	36.0	36.0	32.0	36.0
35	34.48069953897434	36.0	36.0	36.0	32.0	36.0
36	34.45232774249455	36.0	36.0	36.0	32.0	36.0
37	34.44676730936608	36.0	36.0	36.0	32.0	36.0
38	34.015243682276164	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	10.0
21	35.0
22	154.0
23	442.0
24	1325.0
25	3197.0
26	7072.0
27	13480.0
28	23798.0
29	37376.0
30	56598.0
31	81556.0
32	117787.0
33	189041.0
34	470229.0
35	1102767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.535855045126155	18.545462320427813	12.194255115446474	26.724427518999548
2	15.749461249900232	20.738173152571747	36.74137904822716	26.77098654930086
3	17.397542463389698	24.812055079833833	29.12528647822765	28.665115978548815
4	11.947073140488211	16.28653971961174	36.32486043061153	35.44152670928852
5	14.143472857256878	37.00082475323915	33.304923054703565	15.550779334800405
6	33.923393807405596	36.24838410371382	16.634194337034753	13.194027751845837
7	29.92399516168725	30.69596829441874	21.181205834480675	18.19883070941333
8	28.06313895073113	32.82269535893364	19.486007816352767	19.628157873982456
9	27.68884600272615	13.73343842660361	18.5016892365392	40.076026334131036
10	15.853813810960402	26.452953892201343	31.419958666888377	26.273273629949877
11	37.76438705533231	20.89863329744666	22.2862882190677	19.050691428153332
12	24.71214346932919	23.840164798932758	28.565686779408495	22.882004952329552
13	29.520858998009853	19.04354233584861	25.300077771986977	26.135520894154556
14	23.788952487372153	19.68699284327028	24.833148999084028	31.69090567027354
15	25.232080620579307	27.02667905696878	22.121297637100973	25.61994268535094
16	26.01723621886387	25.489460156684263	23.499265987923245	24.99403763652862
17	24.14151549357871	25.75315743665173	24.94360702218472	25.16172004758484
18	25.30662197036209	24.25216355198796	26.1264818003185	24.31473267733145
19	25.85344857074444	24.75285908121729	25.184096705167818	24.209595642870447
20	26.02937375764417	23.78163612799258	24.770057276642	25.418932837721247
21	27.446670128478186	23.959995648189366	23.98099455976162	24.612339663570832
22	26.03793771684821	24.098198891998607	24.66075118142632	25.203112209726868
23	24.412242869617355	23.650864235538034	25.456489866813374	26.48040302803124
24	25.01928858381887	24.863602157280823	24.95876233804241	25.158346920857895
25	25.08485092651891	24.097520604617486	25.17412018235823	25.64350828650538
26	24.919567555651838	25.035489093873643	25.49504197879966	24.549901371674856
27	25.658804905951527	24.450655431779225	24.468756294919597	25.421783367349654
28	24.606531893625835	24.320671280723957	25.725364772774782	25.347432052875423
29	24.72867708820299	24.428848880121926	25.50435370891912	25.33812032275597
30	24.64791208206485	24.691287641243743	25.846987370253395	24.813812906438017
31	25.492619028615515	24.353927459722016	24.43188944505889	25.721564066603577
32	24.944937269344646	24.554984816178845	24.449657746409283	26.05042016806723
33	24.49479113219236	24.105408784952246	25.29089654857873	26.108903534276667
34	25.39061757674576	24.210783363548945	25.466916753132736	24.93168230657256
35	26.301456810675422	24.06811435564728	25.139485916483284	24.490942917194015
36	24.859896468763896	24.913486425777908	24.696893682846273	25.529723422611923
37	25.936303965276746	24.668293368907943	24.65427826490162	24.741124400913687
38	24.88733270938348	24.398893993453278	25.210393040900385	25.503380256262858
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	197.0
1	178.5
2	160.0
3	160.0
4	592.5
5	1025.0
6	1025.0
7	1208.5
8	1392.0
9	1384.5
10	1377.0
11	1377.0
12	1806.0
13	2235.0
14	3034.0
15	3833.0
16	3833.0
17	6144.5
18	8456.0
19	8456.0
20	10031.5
21	11607.0
22	13065.0
23	14523.0
24	14523.0
25	17209.0
26	19895.0
27	19895.0
28	25656.0
29	31417.0
30	38656.0
31	45895.0
32	45895.0
33	59404.0
34	72913.0
35	72913.0
36	81222.5
37	89532.0
38	102592.5
39	115653.0
40	115653.0
41	122091.0
42	128529.0
43	146606.5
44	164684.0
45	164684.0
46	168572.5
47	172461.0
48	172461.0
49	183890.5
50	195320.0
51	197014.5
52	198709.0
53	198709.0
54	189867.5
55	181026.0
56	181026.0
57	176311.0
58	171596.0
59	153845.5
60	136095.0
61	136095.0
62	129683.5
63	123272.0
64	101193.0
65	79114.0
66	79114.0
67	66384.5
68	53655.0
69	53655.0
70	43114.5
71	32574.0
72	25489.5
73	18405.0
74	18405.0
75	13701.5
76	8998.0
77	8998.0
78	8901.5
79	8805.0
80	6850.0
81	4895.0
82	4895.0
83	4657.0
84	4419.0
85	4419.0
86	2788.5
87	1158.0
88	989.5
89	821.0
90	821.0
91	480.0
92	139.0
93	92.5
94	46.0
95	46.0
96	32.5
97	19.0
98	19.0
99	20.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01030941548939793
2	0.0
3	0.0
4	2.3754413570041316E-4
5	0.0
6	1.4252648142024787E-4
7	2.8505296284049575E-4
8	0.002565476665564462
9	0.003563162035506197
10	0.0010451941970818178
11	0.004655865059728098
12	1.9003530856033052E-4
13	4.750882714008263E-5
14	0.0
15	0.0
16	9.501765428016526E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	4.750882714008263E-5
22	4.750882714008263E-5
23	2.8505296284049575E-4
24	0.0
25	1.9003530856033052E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.501765428016526E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2104872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.15830300058286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9026978935997	37.8040612461586
2	13.914573599204408	13.680336502272022
3	4.174237870949655	6.155953501699533
4	1.6880735541303926	3.3193132504485057
5	0.8786685490540002	2.1596927385739524
6	0.5217600030019913	1.538930179269415
7	0.34317153614908275	1.1808811248634459
8	0.24985818491906872	0.9826083489141794
9	0.18557670833791193	0.821037245249328
>10	0.9552632395523222	8.36408637934579
>50	0.07859859808117199	2.7136129022239386
>100	0.08343483434359061	8.984327421171837
>500	0.015247630251030556	5.059351004692584
>1k	0.008837798425756565	7.235808155116989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4915	0.2335058853935061	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3620	0.1719819542470991	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3283	0.15597147950089127	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3145	0.14941526135555985	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2953	0.140293566544664	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2896	0.1375855633976793	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2875	0.13658787802773756	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2813	0.1336423307450524	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2727	0.1295565716110053	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2549	0.12110000038007061	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2545	0.12090996507151028	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2521	0.11976975322014831	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2511	0.11929466494874748	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2430	0.11544644995040078	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2313	0.10988791717501112	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2249	0.10684735223804583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2216	0.10527956094242309	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2181	0.1036167519925202	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2109	0.10019611643843425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.750882714008263E-5
2	0.0	0.0	0.0	0.0	4.750882714008263E-5
3	0.0	0.0	0.0	0.0	4.750882714008263E-5
4	0.0	0.0	0.0	0.0	4.750882714008263E-5
5	0.0	0.0	0.0	0.0	4.750882714008263E-5
6	0.0	0.0	0.0	0.0	1.4252648142024787E-4
7	0.0	0.0	0.0	0.0	1.4252648142024787E-4
8	0.0	0.0	0.0	0.0	1.9003530856033052E-4
9	0.0	0.0	0.0	0.0	2.3754413570041313E-4
10	0.0	0.0	0.0	4.750882714008263E-5	2.3754413570041313E-4
11	0.0	0.0	0.0	4.750882714008263E-5	2.8505296284049575E-4
12	0.0	0.0	0.0	4.750882714008263E-5	4.275794442607436E-4
13	0.0	0.0	0.0	4.750882714008263E-5	4.275794442607436E-4
14	0.0	0.0	0.0	4.750882714008263E-5	4.7508827140082627E-4
15	0.0	0.0	0.0	9.501765428016526E-5	6.176147528210742E-4
16	0.0	0.0	0.0	9.501765428016526E-5	6.176147528210742E-4
17	0.0	0.0	0.0	9.501765428016526E-5	6.176147528210742E-4
18	0.0	0.0	0.0	1.4252648142024787E-4	6.176147528210742E-4
19	0.0	0.0	0.0	1.4252648142024787E-4	6.176147528210742E-4
20	0.0	0.0	0.0	1.4252648142024787E-4	6.651235799611568E-4
21	0.0	0.0	0.0	2.3754413570041313E-4	6.651235799611568E-4
22	0.0	0.0	0.0	3.325617899805784E-4	6.651235799611568E-4
23	0.0	0.0	0.0	8.076500613814047E-4	8.076500613814047E-4
24	0.0	0.0	0.0	0.001140211851361983	8.551588885214872E-4
25	0.0	0.0	0.0	0.0018053354313231399	8.551588885214872E-4
26	0.0	0.0	0.0	0.002327932529864049	9.026677156615699E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTA	45	3.5383314E-4	21.333143	22
ACGATTA	40	0.0044849287	19.99982	32
TCTAGCG	595	0.0	18.823362	28
CTAGCGG	610	0.0	18.0982	29
TGCGTAT	55	0.0013652385	17.45439	23
TAGCGGC	670	0.0	16.477465	30
GTATTAG	460	0.0	16.349623	1
CTAGCGC	60	0.0024429567	15.999858	9
TAGGACG	285	0.0	15.719532	4
CTATTAG	125	5.1364623E-8	15.361689	1
TACTATA	220	0.0	15.272954	2
CATCGTT	625	0.0	15.103866	28
TCTTATA	215	0.0	14.883942	2
CGGCATC	635	0.0	14.86601	25
ACCGTCG	560	0.0	14.857364	8
TATACTG	205	0.0	14.829489	5
AACCGCG	445	0.0	14.741792	7
ATCGTTT	630	0.0	14.730027	29
GTATCAA	4110	0.0	14.483343	1
TCGTTTA	640	0.0	14.249872	30
>>END_MODULE