FastQCFastQC Report
Thu 2 Feb 2017
SRR4062814_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062814_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931660
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26820.287873258484855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19540.20973316445913748No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12390.13298842925530774No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12190.13084172337548033No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12050.12933902925960114No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11250.12075220574029152No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11140.11957151750638645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10790.11581478221668849No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10750.11538544104072301No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10730.11517077045274027No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10700.11484876457076615No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10370.11130669986905094No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10040.10776463516733571No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9820.10540325869952556No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9550.10250520576175859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA400.004486857419.9969166
ACTATAC701.7865648E-518.2858373
GTAGGAC4500.018.1334573
ATCGTTT2550.017.56780229
AACCGCG1550.017.5466217
GGTTAGA550.00135746417.467791
GTCCTAT3800.016.8548871
CATCGTT2700.016.59181428
CCTATTC3000.016.5334453
TCCTATT3400.016.475122
TACCGTC2050.016.3885947
CTATTAG600.002429108816.012141
TATACTG1703.6379788E-1215.997535
CGGCATC2950.015.72807425
GCATCGT3000.015.4651127
TGTAGGA5800.015.4525272
TCGTTTA2800.015.42784630
GTATAGC1153.314126E-715.3159621
CTATACT1052.186036E-615.2365614
AGAACCG2100.015.2357435
Produced by FastQC (version 0.11.5)