##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062814_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931660
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252933473584783	32.0	32.0	32.0	32.0	32.0
2	30.927788034261425	32.0	32.0	32.0	32.0	32.0
3	30.977429534379496	32.0	32.0	32.0	32.0	32.0
4	31.023338986325484	32.0	32.0	32.0	32.0	32.0
5	30.977464955026512	32.0	32.0	32.0	32.0	32.0
6	34.617845565979	36.0	36.0	36.0	32.0	36.0
7	34.546328059592554	36.0	36.0	36.0	32.0	36.0
8	34.53510615460576	36.0	36.0	36.0	32.0	36.0
9	34.62981881802374	36.0	36.0	36.0	32.0	36.0
10	34.400281218470255	36.0	36.0	36.0	32.0	36.0
11	34.63810510271988	36.0	36.0	36.0	32.0	36.0
12	34.47529785544083	36.0	36.0	36.0	32.0	36.0
13	34.54354378206642	36.0	36.0	36.0	32.0	36.0
14	34.46020973316446	36.0	36.0	36.0	32.0	36.0
15	34.41502264774703	36.0	36.0	36.0	32.0	36.0
16	34.4231618830904	36.0	36.0	36.0	32.0	36.0
17	34.36400832921881	36.0	36.0	36.0	32.0	36.0
18	34.38267715690273	36.0	36.0	36.0	32.0	36.0
19	34.366814073803745	36.0	36.0	36.0	32.0	36.0
20	34.343303351007876	36.0	36.0	36.0	32.0	36.0
21	34.312884528690724	36.0	36.0	36.0	32.0	36.0
22	34.29172337548032	36.0	36.0	36.0	32.0	36.0
23	34.25327372646674	36.0	36.0	36.0	32.0	36.0
24	34.233498271901766	36.0	36.0	36.0	32.0	36.0
25	34.20487409570015	36.0	36.0	36.0	32.0	36.0
26	34.16650172809823	36.0	36.0	36.0	32.0	36.0
27	34.164977566923554	36.0	36.0	36.0	32.0	36.0
28	34.14588154476955	36.0	36.0	36.0	32.0	36.0
29	34.10766481334392	36.0	36.0	36.0	32.0	36.0
30	34.07089818174012	36.0	36.0	36.0	32.0	36.0
31	34.09471803018268	36.0	36.0	36.0	32.0	36.0
32	34.042748427537944	36.0	36.0	36.0	32.0	36.0
33	34.01110812957517	36.0	36.0	36.0	32.0	36.0
34	34.02123199450443	36.0	36.0	36.0	32.0	36.0
35	33.980851383551936	36.0	36.0	36.0	32.0	36.0
36	33.92888500096602	36.0	36.0	36.0	32.0	36.0
37	33.921285662151426	36.0	36.0	36.0	32.0	36.0
38	33.32973402314149	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	5.0
11	10.0
12	8.0
13	9.0
14	155.0
15	285.0
16	376.0
17	430.0
18	551.0
19	686.0
20	879.0
21	1228.0
22	1766.0
23	2609.0
24	3887.0
25	5789.0
26	8385.0
27	12172.0
28	16793.0
29	23145.0
30	30916.0
31	42178.0
32	57161.0
33	86062.0
34	195706.0
35	440460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.461648162338	17.32299627190885	11.307325177887659	25.908030387865487
2	16.3950200446104	19.90521632392692	38.14844287796145	25.551320753501226
3	18.713649918524293	24.274179177857498	28.725393584566206	28.286777319052003
4	12.361815517879856	15.496197545175955	36.35082408883307	35.79116284811112
5	14.327038462611815	36.955406383028965	33.67297871107209	15.044576443287127
6	33.685108758070314	36.018590572690535	17.05642110008533	13.239879569153818
7	29.95438250005367	30.620720005152098	21.256574286756972	18.16832320803727
8	27.995418909983943	33.36800278214277	19.60251423272109	19.0340640751522
9	27.891857428522666	13.874389664029435	18.68645072929044	39.54730217815746
10	16.030736732735544	26.71442402436956	31.59069783417449	25.664141408720404
11	37.53683033198553	20.944638308697485	22.437065080068738	19.08146627924825
12	24.911848335127672	23.922402920463316	28.988453607804793	22.177295136604222
13	30.097570977545473	19.372385341487686	25.020638156489287	25.50940552447755
14	23.436916299181153	20.258578120907387	25.258277548531705	31.04622803137976
15	24.993155604513113	27.877464384986776	22.399263918816345	24.730116091683765
16	25.24381590543861	25.546754148761785	24.677765814353176	24.53166413144643
17	23.748844529808178	26.104876433131963	25.655893730024186	24.490385307035673
18	24.749866090740564	24.44973760225676	27.238914513831563	23.56148179317111
19	25.58691241667293	24.865334864798136	25.574675011002928	23.973077707526006
20	25.915362634956278	24.083349255794882	25.455614975558127	24.545673133690713
21	26.902169829346263	24.18553575913971	24.3289458712701	24.583348540243925
22	25.740857824641555	24.7494334948137	25.08606263062359	24.423646049921153
23	23.883082016275942	24.518146462006165	25.883492081553612	25.715279440164284
24	24.736020577297985	25.183572514363746	25.656244900763493	24.424162007574783
25	24.676377315386382	24.599081467676378	25.749286623738843	24.975254593198397
26	24.066423896799904	25.90555593726918	26.144532524863873	23.883487641067042
27	25.109062491949125	25.15479145018764	25.066553882882342	24.66959217498089
28	23.989763726278433	25.15347433256511	25.874178419882586	24.982583521273867
29	24.12189927390302	25.230903992889314	25.81057928884576	24.836617444361906
30	23.948558756410833	25.320783232756494	26.238640827971736	24.492017182860934
31	24.959209611074375	25.06655273982602	24.82889505276988	25.145342596329723
32	24.186006569484103	25.44333283239228	24.889435153180617	25.481225444943
33	23.71396520908364	25.0967994718565	25.884184141739976	25.305051177319886
34	24.86704782842997	25.069830753012763	25.864215952543002	24.19890546601427
35	25.40793892080832	25.011701405886345	25.825646905246526	23.754712768058813
36	23.872069667753436	25.766312008390706	25.573076745531704	24.788541578324157
37	25.02477014486375	25.58393670865694	25.096048048176993	24.29524509830232
38	24.183437313490515	25.565915404663862	25.513853763680938	24.73679351816468
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	465.0
1	370.5
2	276.0
3	276.0
4	531.5
5	787.0
6	787.0
7	935.0
8	1083.0
9	1044.0
10	1005.0
11	1005.0
12	1244.5
13	1484.0
14	1867.5
15	2251.0
16	2251.0
17	3372.0
18	4493.0
19	4493.0
20	5359.5
21	6226.0
22	6460.5
23	6695.0
24	6695.0
25	8139.5
26	9584.0
27	9584.0
28	12422.0
29	15260.0
30	19654.5
31	24049.0
32	24049.0
33	30447.5
34	36846.0
35	36846.0
36	40486.5
37	44127.0
38	49474.5
39	54822.0
40	54822.0
41	57070.5
42	59319.0
43	66132.0
44	72945.0
45	72945.0
46	74973.0
47	77001.0
48	77001.0
49	81006.0
50	85011.0
51	85288.5
52	85566.0
53	85566.0
54	80963.0
55	76360.0
56	76360.0
57	73809.0
58	71258.0
59	63269.0
60	55280.0
61	55280.0
62	52634.0
63	49988.0
64	41397.5
65	32807.0
66	32807.0
67	27426.0
68	22045.0
69	22045.0
70	17665.5
71	13286.0
72	10308.5
73	7331.0
74	7331.0
75	5519.5
76	3708.0
77	3708.0
78	3689.0
79	3670.0
80	2943.5
81	2217.0
82	2217.0
83	2290.5
84	2364.0
85	2364.0
86	1512.0
87	660.0
88	589.0
89	518.0
90	518.0
91	368.5
92	219.0
93	185.5
94	152.0
95	152.0
96	144.0
97	136.0
98	136.0
99	251.0
100	366.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12418693514801536
2	0.05227228817379731
3	0.010089517635188802
4	0.002683382349784256
5	2.1467058798274052E-4
6	5.366764699568512E-4
7	0.0
8	4.2934117596548103E-4
9	1.0733529399137026E-4
10	6.440117639482215E-4
11	0.0024687117618015157
12	0.002898052937766997
13	0.014275594100852242
14	0.011162870575102506
15	0.025867805851920226
16	0.01255822939699032
17	0.023721099972092822
18	0.006332782345490844
19	0.009660176459223322
20	0.006654788227464955
21	0.0070841294034304355
22	0.0076208058733872866
23	0.010840864693128394
24	0.015885623510722795
25	0.01867634115449842
26	0.020179035270377604
27	0.009016164695275102
28	0.0076208058733872866
29	0.011162870575102506
30	0.0036493999957065886
31	0.007298799991413177
32	0.00858682351930962
33	0.010196852929180173
34	0.01309490586694717
35	0.015885623510722795
36	0.016422299980679646
37	0.010196852929180173
38	0.007728141167378658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	931660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.33408522064803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7191462238624	48.48730786205248
2	12.22264092684673	14.504384367498083
3	3.124657339793926	5.561960546539684
4	1.1005799955628262	2.612076289954606
5	0.5117375307657498	1.5181739130529492
6	0.2818323214092921	1.0033357785859203
7	0.1733674696562758	0.7200620153351506
8	0.12096089587407159	0.5741683283326476
9	0.08791493618491464	0.46947170841872016
>10	0.48898545327394166	5.696321821386741
>50	0.07573684070367954	3.2312688791332187
>100	0.08172500936733745	10.41086410628903
>500	0.00835411200252891	3.3920921390901047
>1k	0.002360944696366866	1.818512244330652
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2682	0.287873258484855	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1954	0.20973316445913748	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1239	0.13298842925530774	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1219	0.13084172337548033	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1205	0.12933902925960114	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1125	0.12075220574029152	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1114	0.11957151750638645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1079	0.11581478221668849	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1075	0.11538544104072301	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1073	0.11517077045274027	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1070	0.11484876457076615	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1037	0.11130669986905094	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1004	0.10776463516733571	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	982	0.10540325869952556	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	955	0.10250520576175859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0733529399137024E-4
2	0.0	0.0	0.0	0.0	1.0733529399137024E-4
3	0.0	0.0	0.0	0.0	1.0733529399137024E-4
4	0.0	0.0	0.0	0.0	1.0733529399137024E-4
5	0.0	0.0	0.0	0.0	1.0733529399137024E-4
6	0.0	0.0	0.0	0.0	1.0733529399137024E-4
7	0.0	0.0	0.0	0.0	1.0733529399137024E-4
8	0.0	0.0	0.0	0.0	1.0733529399137024E-4
9	0.0	0.0	0.0	0.0	1.0733529399137024E-4
10	0.0	0.0	0.0	0.0	1.0733529399137024E-4
11	0.0	0.0	0.0	1.0733529399137024E-4	1.0733529399137024E-4
12	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
13	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
14	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
15	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
16	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
17	0.0	0.0	0.0	2.146705879827405E-4	2.146705879827405E-4
18	0.0	0.0	0.0	2.146705879827405E-4	2.146705879827405E-4
19	0.0	0.0	0.0	2.146705879827405E-4	2.146705879827405E-4
20	0.0	0.0	0.0	2.146705879827405E-4	2.146705879827405E-4
21	0.0	0.0	0.0	4.29341175965481E-4	2.146705879827405E-4
22	0.0	0.0	0.0	6.440117639482214E-4	2.146705879827405E-4
23	0.0	0.0	0.0	0.0015026941158791834	2.146705879827405E-4
24	0.0	0.0	0.0	0.0020393705858360346	2.146705879827405E-4
25	0.0	0.0	0.0	0.002683382349784256	2.146705879827405E-4
26	0.0	0.0	0.0	0.004078741171672069	3.220058819741107E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	40	0.0044868574	19.996916	6
ACTATAC	70	1.7865648E-5	18.285837	3
GTAGGAC	450	0.0	18.133457	3
ATCGTTT	255	0.0	17.567802	29
AACCGCG	155	0.0	17.546621	7
GGTTAGA	55	0.001357464	17.46779	1
GTCCTAT	380	0.0	16.854887	1
CATCGTT	270	0.0	16.591814	28
CCTATTC	300	0.0	16.533445	3
TCCTATT	340	0.0	16.47512	2
TACCGTC	205	0.0	16.388594	7
CTATTAG	60	0.0024291088	16.01214	1
TATACTG	170	3.6379788E-12	15.99753	5
CGGCATC	295	0.0	15.728074	25
GCATCGT	300	0.0	15.46511	27
TGTAGGA	580	0.0	15.452527	2
TCGTTTA	280	0.0	15.427846	30
GTATAGC	115	3.314126E-7	15.315962	1
CTATACT	105	2.186036E-6	15.236561	4
AGAACCG	210	0.0	15.235743	5
>>END_MODULE