FastQCFastQC Report
Thu 2 Feb 2017
SRR4062814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931660
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31640.33960887018869546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24340.2612541055749952No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14440.15499216452353862No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13290.14264860571453103No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12330.1323444174913595No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12280.13180774102140266No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11910.12783633514372197No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11710.12568962926389457No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11190.1201081939763433No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11020.11828349397849001No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10860.11656612927462809No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10370.11130669986905094No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10240.10991134104716314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.1042225704656205No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9620.10325655281969816No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9370.10057317046991393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGTT2500.019.83923328
ATCGTTT2600.019.69154729
CGCGTAA802.7946262E-618.0002710
GCTTAAC450.00884554517.7790011
CGTTTAT2700.017.77709231
CTAGGAC1453.6379788E-1217.6563853
GTTAAGT652.098762E-417.2319551
TGTACGT652.0996231E-417.2310310
GTATAGT753.2307216E-517.067841
CGTGCGA753.233699E-517.0660132
CCTACAC1700.016.942343
TCGCGTA854.9786267E-616.942349
ACCCGTT1151.8402716E-816.6950130
TCGTTTA2900.016.55108530
ACCGTCG2350.016.3424268
GCATCGT3050.016.2616727
TTAGGAC3150.016.2550853
AGACGGA3600.016.001966
TACCGTC2400.016.0019597
GTGTTAA805.6157216E-516.00111
Produced by FastQC (version 0.11.5)