##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931660
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18591975613421	32.0	32.0	32.0	32.0	32.0
2	31.313524247042913	32.0	32.0	32.0	32.0	32.0
3	31.397106240473992	32.0	32.0	32.0	32.0	32.0
4	31.496969924650625	32.0	32.0	32.0	32.0	32.0
5	31.421543266857007	32.0	32.0	32.0	32.0	32.0
6	34.95508876628813	36.0	36.0	36.0	36.0	36.0
7	34.983488611725306	36.0	36.0	36.0	36.0	36.0
8	34.91596505162828	36.0	36.0	36.0	36.0	36.0
9	35.02950325225941	36.0	36.0	36.0	36.0	36.0
10	34.87387136938368	36.0	36.0	36.0	32.0	36.0
11	35.03676448489792	36.0	36.0	36.0	36.0	36.0
12	34.94121245948093	36.0	36.0	36.0	36.0	36.0
13	34.9928385891849	36.0	36.0	36.0	36.0	36.0
14	34.94036236395252	36.0	36.0	36.0	32.0	36.0
15	34.916258076980874	36.0	36.0	36.0	32.0	36.0
16	34.924648476912175	36.0	36.0	36.0	36.0	36.0
17	34.888360560719576	36.0	36.0	36.0	32.0	36.0
18	34.8813021917867	36.0	36.0	36.0	32.0	36.0
19	34.87226563338557	36.0	36.0	36.0	32.0	36.0
20	34.863982568748256	36.0	36.0	36.0	32.0	36.0
21	34.84999892664706	36.0	36.0	36.0	32.0	36.0
22	34.83527896442909	36.0	36.0	36.0	32.0	36.0
23	34.773223064207976	36.0	36.0	36.0	32.0	36.0
24	34.757559624755814	36.0	36.0	36.0	32.0	36.0
25	34.732740484726186	36.0	36.0	36.0	32.0	36.0
26	34.665768628040276	36.0	36.0	36.0	32.0	36.0
27	34.64584934418135	36.0	36.0	36.0	32.0	36.0
28	34.62295150591417	36.0	36.0	36.0	32.0	36.0
29	34.58496232531181	36.0	36.0	36.0	32.0	36.0
30	34.55388124423073	36.0	36.0	36.0	32.0	36.0
31	34.54757529570873	36.0	36.0	36.0	32.0	36.0
32	34.51648992121589	36.0	36.0	36.0	32.0	36.0
33	34.48460382542988	36.0	36.0	36.0	32.0	36.0
34	34.46411459115986	36.0	36.0	36.0	32.0	36.0
35	34.43575982654617	36.0	36.0	36.0	32.0	36.0
36	34.40435244617135	36.0	36.0	36.0	32.0	36.0
37	34.38971083871799	36.0	36.0	36.0	32.0	36.0
38	33.96431960157139	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	22.0
22	57.0
23	187.0
24	628.0
25	1482.0
26	3171.0
27	6271.0
28	10620.0
29	16832.0
30	25646.0
31	36853.0
32	53129.0
33	84432.0
34	207530.0
35	484796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.792858070631375	18.53582504355555	12.075863408936966	26.59545347687611
2	15.477212717085632	20.78623102848679	37.43135907949252	26.305197174935063
3	17.789000279071765	24.919713200094453	29.059528154047616	28.23175836678617
4	11.703234130157343	16.350437983077462	36.4876773318936	35.45865055487159
5	13.795283467448927	37.481524892691425	33.41351288400584	15.309678755853806
6	33.744249499279775	36.381375998488714	16.703554308555287	13.170820193676219
7	29.586886146818138	30.845183200666344	21.26074430905828	18.30718634345724
8	27.774670927285744	33.68246938440351	19.4464987822458	19.09636090606494
9	27.7017750818736	13.742313112978588	18.491357518470533	40.06455428667728
10	15.780570427584548	27.03123054531091	31.763254949272905	25.424944077831636
11	37.62481027550037	20.810335611100736	22.4295684693446	19.1352856440543
12	24.690524517070124	23.963754901213644	29.041159997724485	22.304560583991748
13	30.141747293534753	19.342398176154767	25.08678077148482	25.429073758825666
14	23.35293991370242	20.141145911598652	25.30043148788185	31.205482686817078
15	24.969087435330486	27.95751669063822	22.366099220745767	24.707296653285532
16	25.328553334907582	25.558143528755124	24.541248953480885	24.572054182856405
17	23.748041130884655	26.168774016272028	25.5435459287723	24.539638924071014
18	24.801322370821975	24.374235236030312	27.171500332739413	23.652942060408304
19	25.56393963463066	24.801751711997937	25.635317605134922	23.99899104823648
20	25.76755468733229	24.029366936436038	25.59055878754052	24.612519588691153
21	26.917573919212927	24.078230339641436	24.51755416949764	24.486641571647997
22	25.603546358113476	24.853057982525815	25.12869501749565	24.414700641865057
23	23.863799806366714	24.303982791968725	26.014696380002107	25.817521021662454
24	24.759246935577355	24.946976364768265	25.743189575596247	24.550587124058133
25	24.69994354151416	24.619549233731693	25.789828456364887	24.89067876838926
26	24.113839812807246	25.60923512869502	26.194212480947986	24.08271257754975
27	25.135296428517762	24.81006978955797	25.251540801452894	24.803092980471376
28	24.00596784234592	24.915741794216775	26.153210398643285	24.925079964794023
29	24.218706395036815	24.87795977073181	25.979434557671254	24.923899276560117
30	23.887899018955412	25.10132451752785	26.509885580576604	24.50089088294013
31	25.00257604705579	24.818066676684627	25.06504518815877	25.11431208810081
32	24.364575059571088	25.23527896442908	25.056243694051478	25.34390228194835
33	23.79301461906704	24.714273447394973	26.11832642809609	25.3743855054419
34	24.83491831784127	24.755490200287657	26.22211965738574	24.187471824485325
35	25.47120194062212	24.67971148272975	25.999506257647642	23.849580319000495
36	24.095592812828713	25.470021252388207	25.64487044630015	24.789515488482923
37	25.135027799841144	25.278427752613613	25.126118970439858	24.46042547710538
38	24.247176273722467	25.26278391557426	25.700175708064858	24.78986410263841
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	274.0
1	204.5
2	135.0
3	135.0
4	444.5
5	754.0
6	754.0
7	893.0
8	1032.0
9	983.5
10	935.0
11	935.0
12	1216.5
13	1498.0
14	1923.5
15	2349.0
16	2349.0
17	3618.5
18	4888.0
19	4888.0
20	5683.0
21	6478.0
22	6783.5
23	7089.0
24	7089.0
25	8299.0
26	9509.0
27	9509.0
28	12361.0
29	15213.0
30	19284.0
31	23355.0
32	23355.0
33	30261.5
34	37168.0
35	37168.0
36	40788.0
37	44408.0
38	49606.5
39	54805.0
40	54805.0
41	57112.5
42	59420.0
43	66129.5
44	72839.0
45	72839.0
46	74952.5
47	77066.0
48	77066.0
49	81534.0
50	86002.0
51	85649.5
52	85297.0
53	85297.0
54	81030.5
55	76764.0
56	76764.0
57	73923.0
58	71082.0
59	63233.5
60	55385.0
61	55385.0
62	52903.5
63	50422.0
64	41429.0
65	32436.0
66	32436.0
67	27288.5
68	22141.0
69	22141.0
70	17817.5
71	13494.0
72	10476.5
73	7459.0
74	7459.0
75	5582.5
76	3706.0
77	3706.0
78	3598.0
79	3490.0
80	2719.5
81	1949.0
82	1949.0
83	1925.5
84	1902.0
85	1902.0
86	1174.0
87	446.0
88	407.5
89	369.0
90	369.0
91	210.5
92	52.0
93	39.0
94	26.0
95	26.0
96	17.5
97	9.0
98	9.0
99	11.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009767511753214693
2	0.0
3	0.0
4	3.2200588197411076E-4
5	1.0733529399137026E-4
6	2.1467058798274052E-4
7	4.2934117596548103E-4
8	0.002898052937766997
9	0.0044007470536461795
10	8.586823519309621E-4
11	0.004937423523603031
12	3.2200588197411076E-4
13	2.1467058798274052E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0733529399137026E-4
22	0.0
23	2.1467058798274052E-4
24	0.0
25	2.1467058798274052E-4
26	0.0
27	2.1467058798274052E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0733529399137026E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	931660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.576104173010535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56654782015133	46.38707950152868
2	12.85063970997661	14.797795412628783
3	3.415535912956998	5.899597545932124
4	1.2009431400633916	2.7658250935260886
5	0.5840026346070726	1.6812298263724708
6	0.2919835961816359	1.0086766770338464
7	0.1951678102683461	0.7865901522660056
8	0.12167881951279014	0.5604633910333883
9	0.09033267662013367	0.4680903239376921
>10	0.5040702817739414	5.650921652203049
>50	0.0781236437266151	3.1991862593583886
>100	0.08879713228537557	10.93889950943475
>500	0.00974145750060834	3.853536012453022
>1k	0.002435364375152085	2.0021086422916277
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3164	0.33960887018869546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2434	0.2612541055749952	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1444	0.15499216452353862	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1329	0.14264860571453103	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1233	0.1323444174913595	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1228	0.13180774102140266	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1191	0.12783633514372197	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1171	0.12568962926389457	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1119	0.1201081939763433	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1102	0.11828349397849001	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1086	0.11656612927462809	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1037	0.11130669986905094	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1024	0.10991134104716314	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	971	0.1042225704656205	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	962	0.10325655281969816	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	937	0.10057317046991393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.0733529399137024E-4	0.0
12	0.0	0.0	0.0	1.0733529399137024E-4	2.146705879827405E-4
13	0.0	0.0	0.0	1.0733529399137024E-4	3.220058819741107E-4
14	0.0	0.0	0.0	1.0733529399137024E-4	3.220058819741107E-4
15	0.0	0.0	0.0	1.0733529399137024E-4	4.29341175965481E-4
16	0.0	0.0	0.0	1.0733529399137024E-4	4.29341175965481E-4
17	0.0	0.0	0.0	2.146705879827405E-4	4.29341175965481E-4
18	0.0	0.0	0.0	2.146705879827405E-4	4.29341175965481E-4
19	0.0	0.0	0.0	2.146705879827405E-4	4.29341175965481E-4
20	0.0	0.0	0.0	3.220058819741107E-4	5.366764699568512E-4
21	0.0	0.0	0.0	4.29341175965481E-4	5.366764699568512E-4
22	0.0	0.0	0.0	7.513470579395917E-4	5.366764699568512E-4
23	0.0	0.0	0.0	0.0016100294098705535	5.366764699568512E-4
24	0.0	0.0	0.0	0.002146705879827405	5.366764699568512E-4
25	0.0	0.0	0.0	0.0027907176437756264	5.366764699568512E-4
26	0.0	0.0	0.0	0.004078741171672069	6.440117639482214E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGTT	250	0.0	19.839233	28
ATCGTTT	260	0.0	19.691547	29
CGCGTAA	80	2.7946262E-6	18.00027	10
GCTTAAC	45	0.008845545	17.779001	1
CGTTTAT	270	0.0	17.777092	31
CTAGGAC	145	3.6379788E-12	17.656385	3
GTTAAGT	65	2.098762E-4	17.231955	1
TGTACGT	65	2.0996231E-4	17.23103	10
GTATAGT	75	3.2307216E-5	17.06784	1
CGTGCGA	75	3.233699E-5	17.06601	32
CCTACAC	170	0.0	16.94234	3
TCGCGTA	85	4.9786267E-6	16.94234	9
ACCCGTT	115	1.8402716E-8	16.69501	30
TCGTTTA	290	0.0	16.551085	30
ACCGTCG	235	0.0	16.342426	8
GCATCGT	305	0.0	16.26167	27
TTAGGAC	315	0.0	16.255085	3
AGACGGA	360	0.0	16.00196	6
TACCGTC	240	0.0	16.001959	7
GTGTTAA	80	5.6157216E-5	16.0011	1
>>END_MODULE