FastQCFastQC Report
Thu 2 Feb 2017
SRR4062813_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062813_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128886
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40770.3611524990123006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT29450.2608766518496996No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16270.1441243845702755No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16210.14359288714715215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16070.14235272649319772No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15080.13358301901166283No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14560.12897670801126068No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13770.12197865860680353No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13420.11887825697191744No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13260.11746093051025525No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13120.11622076985630082No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13090.11595502114473916No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12800.11338611693297641No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA12750.11294320241370696No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12280.10877980593257423No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12270.10869122302872036No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11490.10178175652811709No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11490.10178175652811709No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11480.10169317362426322No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11400.1009845103934321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA400.004482676320.00058232
TCTAGCG3450.019.47796428
CATCGTT2850.018.52603328
GTACTAA450.0088013717.7948511
CCTATAT550.001366791717.4504133
TGTAGGA3600.017.3346042
CTAGCGG3900.017.2312729
GTCCTAC3350.016.7324711
TCTAGAT2650.016.3030832
GTCCTAT3100.016.0153681
GTCATAC703.669404E-416.0153661
GTATTAA1407.3305273E-1016.0153661
TAGCGGC4200.016.00046530
CGGTCCA4200.015.99975810
ACCGGTC600.002443562315.9983398
CCTATTC3100.015.9962133
TAGGACG2600.015.9955044
CGGCATC3350.015.76095525
TAGCGTA2150.015.6262857
CAAGACG3500.015.5384894
Produced by FastQC (version 0.11.5)