##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062813_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128886
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192619981114124	32.0	32.0	32.0	32.0	32.0
2	30.794975754859216	32.0	32.0	32.0	32.0	32.0
3	30.853249132330458	32.0	32.0	32.0	32.0	32.0
4	30.911929991159425	32.0	32.0	32.0	32.0	32.0
5	30.83006344307574	32.0	32.0	32.0	32.0	32.0
6	34.46882147533055	36.0	36.0	36.0	32.0	36.0
7	34.38529222614152	36.0	36.0	36.0	32.0	36.0
8	34.36789808714077	36.0	36.0	36.0	32.0	36.0
9	34.5038799311888	36.0	36.0	36.0	32.0	36.0
10	34.2103365618849	36.0	36.0	36.0	32.0	36.0
11	34.512534480895326	36.0	36.0	36.0	32.0	36.0
12	34.31318574240446	36.0	36.0	36.0	32.0	36.0
13	34.407857835069265	36.0	36.0	36.0	32.0	36.0
14	34.30477656734161	36.0	36.0	36.0	32.0	36.0
15	34.241305145072225	36.0	36.0	36.0	32.0	36.0
16	34.26258630189408	36.0	36.0	36.0	32.0	36.0
17	34.18141247211853	36.0	36.0	36.0	32.0	36.0
18	34.218071621049425	36.0	36.0	36.0	32.0	36.0
19	34.19526418079416	36.0	36.0	36.0	32.0	36.0
20	34.16314224819867	36.0	36.0	36.0	32.0	36.0
21	34.152086215968666	36.0	36.0	36.0	32.0	36.0
22	34.12160572458158	36.0	36.0	36.0	32.0	36.0
23	34.06826464319692	36.0	36.0	36.0	32.0	36.0
24	34.06039670967662	36.0	36.0	36.0	32.0	36.0
25	34.04164370893075	36.0	36.0	36.0	32.0	36.0
26	33.98448647604807	36.0	36.0	36.0	32.0	36.0
27	33.99585166261252	36.0	36.0	36.0	32.0	36.0
28	33.96805700487029	36.0	36.0	36.0	32.0	36.0
29	33.92789617375005	36.0	36.0	36.0	32.0	36.0
30	33.90605162965968	36.0	36.0	36.0	32.0	36.0
31	33.91353245589014	36.0	36.0	36.0	32.0	36.0
32	33.86562150651173	36.0	36.0	36.0	32.0	36.0
33	33.82410181364637	36.0	36.0	36.0	32.0	36.0
34	33.828027807945176	36.0	36.0	36.0	32.0	36.0
35	33.78406411276249	36.0	36.0	36.0	32.0	36.0
36	33.729215350354245	36.0	36.0	36.0	27.0	36.0
37	33.72759428321372	36.0	36.0	36.0	27.0	36.0
38	33.10886573134931	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	0.0
9	6.0
10	4.0
11	10.0
12	5.0
13	7.0
14	153.0
15	315.0
16	380.0
17	483.0
18	588.0
19	787.0
20	1067.0
21	1543.0
22	2323.0
23	3559.0
24	5407.0
25	8317.0
26	12098.0
27	17254.0
28	23487.0
29	31497.0
30	42332.0
31	56217.0
32	75550.0
33	111868.0
34	244446.0
35	489180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94737458271102	16.909649175710147	11.503340085639582	25.63963615593925
2	16.659738735760442	19.529763366105843	37.590391772276995	26.220106125856724
3	18.87966051392451	23.86236284789128	28.48095041926353	28.777026218920675
4	12.416453102563036	15.128665543997158	35.732692449087885	36.722188904351924
5	14.582973464967644	36.64846286379924	33.2764630586818	15.492100612551324
6	34.56224831293262	35.33425105546904	16.714590187459805	13.388910444138538
7	30.618530674072204	30.492299924261552	20.548860158474955	18.340309243191292
8	28.8298766654767	32.58589700774483	19.127171065860797	19.45705526091767
9	28.039600241655016	13.780182516861817	18.31652909692953	39.86368814455364
10	16.22845986423724	26.43795050142575	31.157668523077277	26.175921111259736
11	38.09092278118827	20.824452771355425	21.89738410977349	19.187240337682816
12	25.271468080243643	23.618564536674988	28.554065953145734	22.555901429935634
13	30.007778557405402	19.102648607655944	25.06954632759953	25.820026507339133
14	23.860054465787016	19.785308412076024	24.942282920882864	31.412354201254093
15	25.42739453909613	27.086912490331294	22.557305644576086	24.928387325996486
16	25.824395844665766	25.37115752954997	24.079038525256784	24.72540810052748
17	23.97977446060503	25.675129709960025	25.36627115760823	24.978824671826715
18	25.0903141106164	24.19913220497538	26.72544687545954	23.98510680894867
19	25.712592387852247	24.816774613062886	25.490321146065604	23.980311853019266
20	25.962984814419126	23.87401513420042	25.207603598048962	24.955396453331492
21	27.498965731380732	23.745390085372563	24.19506439041935	24.560579792827358
22	26.101334854696795	24.18604692366842	24.94675818471101	24.765860036923776
23	24.33817575528848	23.96298993421181	25.718345745029563	25.980488565470143
24	24.956189699928238	24.99047602172353	25.278233058393063	24.77510121995517
25	25.03515077845574	24.249297205922872	25.49762604887531	25.21792596674608
26	24.423161261456816	25.39419418000434	25.989660625766923	24.19298393277192
27	25.440736645901108	24.59248470931757	25.07228966895584	24.894488975825485
28	24.35545468316887	24.605007534423144	25.979895802604315	25.059641979803672
29	24.21884551258086	24.91526234565167	25.819868920195116	25.046023221572355
30	24.41575859475558	25.229413468667865	25.935968860537695	24.41885907603886
31	25.09284031112135	24.987774844527912	24.695877110611082	25.22350773373966
32	24.624983500425667	25.028857644773243	24.71746970500976	25.628689149791327
33	24.222024371121417	24.537404931807814	25.522792003862527	25.717778693208242
34	25.131295448424456	24.86002239641657	25.572402086552227	24.436280068606745
35	25.80259623358306	24.66129535257887	25.55319691825727	23.9829114955808
36	24.30428507766687	25.574295381273306	24.99904760301613	25.1223719380437
37	25.47819115243381	25.31270348206257	24.802686073964484	24.406419291539134
38	24.552386166427034	25.225390430175253	25.27393674273732	24.94828666066039
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	126.5
2	167.0
3	167.0
4	467.0
5	767.0
6	767.0
7	995.5
8	1224.0
9	1274.0
10	1324.0
11	1324.0
12	1728.0
13	2132.0
14	2666.5
15	3201.0
16	3201.0
17	4644.0
18	6087.0
19	6087.0
20	7084.5
21	8082.0
22	8288.0
23	8494.0
24	8494.0
25	10070.0
26	11646.0
27	11646.0
28	15158.5
29	18671.0
30	22698.0
31	26725.0
32	26725.0
33	33393.5
34	40062.0
35	40062.0
36	43982.5
37	47903.0
38	54635.0
39	61367.0
40	61367.0
41	64153.5
42	66940.0
43	76084.5
44	85229.0
45	85229.0
46	88102.5
47	90976.0
48	90976.0
49	96142.5
50	101309.0
51	101685.0
52	102061.0
53	102061.0
54	98188.5
55	94316.0
56	94316.0
57	92283.0
58	90250.0
59	81373.5
60	72497.0
61	72497.0
62	68908.0
63	65319.0
64	54396.5
65	43474.0
66	43474.0
67	36597.5
68	29721.0
69	29721.0
70	23997.5
71	18274.0
72	14577.5
73	10881.0
74	10881.0
75	8291.0
76	5701.0
77	5701.0
78	5494.5
79	5288.0
80	4083.5
81	2879.0
82	2879.0
83	2838.0
84	2797.0
85	2797.0
86	1905.0
87	1013.0
88	861.5
89	710.0
90	710.0
91	511.0
92	312.0
93	263.5
94	215.0
95	215.0
96	206.0
97	197.0
98	197.0
99	393.0
100	589.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12135857827982631
2	0.05341549102389435
3	0.010718531366320425
4	0.002568904211762747
5	8.858290385388781E-5
6	3.5433161541555125E-4
7	8.858290385388781E-5
8	4.429145192694391E-4
9	3.5433161541555125E-4
10	4.429145192694391E-4
11	0.001417326461662205
12	0.0021259896924933075
13	0.01257877234725207
14	0.009212622000804332
15	0.021702811444202514
16	0.010629948462466537
17	0.018956741424731992
18	0.004251979384986615
19	0.007529546827580464
20	0.004606311000402166
21	0.006112220365918259
22	0.006555134885187699
23	0.009921285231635435
24	0.0138189330012065
25	0.015679173982138143
26	0.016919334636092572
27	0.008326792962265454
28	0.005935054558210483
29	0.009566953616219884
30	0.002568904211762747
31	0.005846471654356596
32	0.006112220365918259
33	0.008061044250703793
34	0.011870109116420967
35	0.01541342527057648
36	0.013730350097352611
37	0.008946873289242669
38	0.005935054558210483
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1128886.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.964462387935676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06318720016644	39.52582630369559
2	14.139965413818633	14.695514018261793
3	4.704650900183384	7.334239642528415
4	1.9940232557818744	4.1447338590298495
5	0.9750297869609844	2.5333449345825505
6	0.5357442651867096	1.6703797630708208
7	0.32820905281837587	1.1938644886392338
8	0.21040307256509366	0.8746786040491928
9	0.15641432498492036	0.7315187676851916
>10	0.6988504045057633	6.705207354573425
>50	0.0824861058981432	3.0555958566084347
>100	0.09580937200683981	10.518266438521682
>500	0.010607465141806762	3.59380827148474
>1k	0.004619379981109397	3.4230216972690166
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4077	0.3611524990123006	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2945	0.2608766518496996	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1627	0.1441243845702755	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1621	0.14359288714715215	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1607	0.14235272649319772	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1508	0.13358301901166283	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1456	0.12897670801126068	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1377	0.12197865860680353	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1342	0.11887825697191744	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1326	0.11746093051025525	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1312	0.11622076985630082	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1309	0.11595502114473916	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1280	0.11338611693297641	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1275	0.11294320241370696	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1228	0.10877980593257423	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1227	0.10869122302872036	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1149	0.10178175652811709	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1149	0.10178175652811709	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1148	0.10169317362426322	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1140	0.1009845103934321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.7716580770777562E-4	0.0
8	0.0	0.0	0.0	1.7716580770777562E-4	0.0
9	0.0	0.0	0.0	1.7716580770777562E-4	0.0
10	0.0	0.0	0.0	1.7716580770777562E-4	0.0
11	0.0	0.0	0.0	1.7716580770777562E-4	0.0
12	0.0	0.0	0.0	1.7716580770777562E-4	8.858290385388781E-5
13	0.0	0.0	0.0	1.7716580770777562E-4	2.6574871156166344E-4
14	0.0	0.0	0.0	1.7716580770777562E-4	2.6574871156166344E-4
15	0.0	0.0	0.0	1.7716580770777562E-4	2.6574871156166344E-4
16	0.0	0.0	0.0	3.5433161541555125E-4	2.6574871156166344E-4
17	0.0	0.0	0.0	3.5433161541555125E-4	2.6574871156166344E-4
18	0.0	0.0	0.0	4.4291451926943906E-4	2.6574871156166344E-4
19	0.0	0.0	0.0	4.4291451926943906E-4	2.6574871156166344E-4
20	0.0	0.0	0.0	8.858290385388781E-4	2.6574871156166344E-4
21	0.0	0.0	0.0	9.744119423927659E-4	2.6574871156166344E-4
22	0.0	0.0	0.0	0.0013287435578083172	2.6574871156166344E-4
23	0.0	0.0	0.0	0.0020374067886394197	2.6574871156166344E-4
24	0.0	0.0	0.0	0.0030118187310321856	2.6574871156166344E-4
25	0.0	0.0	0.0	0.0034547332503016247	2.6574871156166344E-4
26	0.0	0.0	0.0	0.003809064865717176	4.4291451926943906E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	40	0.0044826763	20.000582	32
TCTAGCG	345	0.0	19.477964	28
CATCGTT	285	0.0	18.526033	28
GTACTAA	45	0.00880137	17.794851	1
CCTATAT	55	0.0013667917	17.450413	3
TGTAGGA	360	0.0	17.334604	2
CTAGCGG	390	0.0	17.23127	29
GTCCTAC	335	0.0	16.732471	1
TCTAGAT	265	0.0	16.303083	2
GTCCTAT	310	0.0	16.015368	1
GTCATAC	70	3.669404E-4	16.015366	1
GTATTAA	140	7.3305273E-10	16.015366	1
TAGCGGC	420	0.0	16.000465	30
CGGTCCA	420	0.0	15.999758	10
ACCGGTC	60	0.0024435623	15.998339	8
CCTATTC	310	0.0	15.996213	3
TAGGACG	260	0.0	15.995504	4
CGGCATC	335	0.0	15.760955	25
TAGCGTA	215	0.0	15.626285	7
CAAGACG	350	0.0	15.538489	4
>>END_MODULE