FastQCFastQC Report
Thu 2 Feb 2017
SRR4062813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128886
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT46810.4146565729400488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34660.3070283447575752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20370.18044337515036948No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17280.15307125785951814No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16410.14536454522422992No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15550.13774641549279557No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15340.1358861745118639No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14610.1294196225305301No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14400.12755938154959845No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14060.12454756281856628No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13970.12375031668388128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.12339598506846572No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13840.12259873893378075No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13290.1177266792218169No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12620.11179162466360643No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12570.11134871014433698No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12390.109754217874967No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11710.10373058041290263No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11690.10355341460519486No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11590.10266758556665599No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT11390.10089592748957822No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGT400.00448213920.0009984
CGAATGT751.5090773E-619.19925523
TCTAGCG3400.018.82279828
ATAGACG450.008849896517.7778763
TTCGATA550.001364979417.45386912
TAGCGGC3600.017.3326630
ACCGAAC652.0988505E-417.2323938
CTAGCGG3650.017.09522829
GTAGGAC4150.016.963953
GATATAC2550.016.942021
TCGCGTA1900.016.843699
CATCGTT3150.016.76125528
ACGAACG1850.016.43179515
CAAGACG4100.016.391064
ATCGTTT3250.016.24552329
AAGACGG4350.016.1861485
CCGTCCA600.002440335216.0015079
GCGTAAC1900.016.00079711
CTAAACG600.002442500815.99938112
CGAACGA1900.015.9993816
Produced by FastQC (version 0.11.5)