##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1128886
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16181881961509	32.0	32.0	32.0	32.0	32.0
2	31.294448686581283	32.0	32.0	32.0	32.0	32.0
3	31.37518757429891	32.0	32.0	32.0	32.0	32.0
4	31.471611836801944	32.0	32.0	32.0	32.0	32.0
5	31.38863534493297	32.0	32.0	32.0	32.0	32.0
6	34.90864179376837	36.0	36.0	36.0	36.0	36.0
7	34.94162298053125	36.0	36.0	36.0	36.0	36.0
8	34.88182509128468	36.0	36.0	36.0	32.0	36.0
9	34.98654691439171	36.0	36.0	36.0	36.0	36.0
10	34.83572477646104	36.0	36.0	36.0	32.0	36.0
11	34.99948887664476	36.0	36.0	36.0	36.0	36.0
12	34.902728884936124	36.0	36.0	36.0	32.0	36.0
13	34.96174281548358	36.0	36.0	36.0	36.0	36.0
14	34.89940968352872	36.0	36.0	36.0	32.0	36.0
15	34.871825853097654	36.0	36.0	36.0	32.0	36.0
16	34.876650963870574	36.0	36.0	36.0	32.0	36.0
17	34.83993600771026	36.0	36.0	36.0	32.0	36.0
18	34.84210806051275	36.0	36.0	36.0	32.0	36.0
19	34.829235192924706	36.0	36.0	36.0	32.0	36.0
20	34.81741203274733	36.0	36.0	36.0	32.0	36.0
21	34.80131208997189	36.0	36.0	36.0	32.0	36.0
22	34.77735661528268	36.0	36.0	36.0	32.0	36.0
23	34.7222589349146	36.0	36.0	36.0	32.0	36.0
24	34.70526430481023	36.0	36.0	36.0	32.0	36.0
25	34.67847594885578	36.0	36.0	36.0	32.0	36.0
26	34.62388496269774	36.0	36.0	36.0	32.0	36.0
27	34.60058588732609	36.0	36.0	36.0	32.0	36.0
28	34.56993797425072	36.0	36.0	36.0	32.0	36.0
29	34.53493178230574	36.0	36.0	36.0	32.0	36.0
30	34.50797246134685	36.0	36.0	36.0	32.0	36.0
31	34.50176457144477	36.0	36.0	36.0	32.0	36.0
32	34.46877098307535	36.0	36.0	36.0	32.0	36.0
33	34.42411722707165	36.0	36.0	36.0	32.0	36.0
34	34.4065255481953	36.0	36.0	36.0	32.0	36.0
35	34.36683686395261	36.0	36.0	36.0	32.0	36.0
36	34.33783039208565	36.0	36.0	36.0	32.0	36.0
37	34.316597070031875	36.0	36.0	36.0	32.0	36.0
38	33.88734469202382	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	17.0
22	85.0
23	278.0
24	797.0
25	1867.0
26	3989.0
27	7874.0
28	13903.0
29	21416.0
30	32907.0
31	46824.0
32	66981.0
33	105392.0
34	253699.0
35	572851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35212902139873	18.094445010431386	12.170303994046803	26.383121974123075
2	15.663671973963714	20.44537712399658	36.97689580701683	26.91405509502288
3	17.968421966434168	24.52825174552612	28.77597915112775	28.727347136911963
4	11.970406171050348	15.931929232764395	35.67275291349687	36.42491168268839
5	14.022142182647318	37.21048892447953	33.04346054428879	15.723908348584356
6	34.55589768302146	35.76983994812576	16.337905400377718	13.33635696847506
7	30.220803310898077	30.668593063200806	20.60878146265584	18.501822163245276
8	28.52736388923339	32.97450525308718	18.997395602643373	19.50073525503605
9	27.86946819753397	13.669554833686048	18.14974235046184	40.311234618318146
10	16.034916208540544	26.666926512613454	31.13669018863044	26.161467090215563
11	38.112356445046046	20.78291266401851	21.79794053343444	19.306790357501
12	24.935732875535262	23.750755171931168	28.574820043193178	22.738691909340393
13	30.000841538332075	19.04604986336075	25.129309008446388	25.82379958986079
14	23.794430970000512	19.752835981666884	24.846441536169287	31.60629151216332
15	25.268539073033057	27.286457622824628	22.394023842974402	25.05097946116791
16	25.669045119742044	25.500826036310166	23.9677203612414	24.86240848270639
17	23.95724634728396	25.707644527436784	25.24550751803105	25.08960160724821
18	25.038046357205246	24.13609523016496	26.765501565259914	24.060356847369885
19	25.722437872380382	24.67955134530856	25.452702930145293	24.14530785216576
20	26.017596108021536	23.75217692486221	25.299100174862655	24.931126792253604
21	27.383730097565557	23.73259561229606	24.34630014474498	24.537374145393407
22	25.891542635837457	24.172857135264323	25.179513254659902	24.75608697423832
23	24.353453812308274	23.742938816511543	25.82083351419058	26.08277385698961
24	24.847858862630947	24.93192403838829	25.403273669794824	24.81694342918594
25	25.067300920820102	24.2182330352516	25.495068142459154	25.219397901469147
26	24.493194612383014	25.16872843558024	26.082904813156343	24.255172138880397
27	25.42900776341945	24.365568118602088	25.200463466574067	25.004960651404396
28	24.369334016012246	24.47625358096389	26.004308672443454	25.150103730580415
29	24.36534778533882	24.583970392050215	25.910853708877603	25.139828113733365
30	24.333280774143713	24.90464050400129	26.22363994238568	24.538438779469317
31	24.989325760085606	24.751569246141774	24.836431668033796	25.422673325738828
32	24.591411355973943	24.78930556318353	24.924394491560705	25.694888589281824
33	24.212896607806282	24.37243441764713	25.688776368915907	25.725892605630683
34	25.048056225340737	24.62303545264978	25.841847626775422	24.48706069523406
35	25.789938045117044	24.446312559461276	25.62517384394881	24.13857555147287
36	24.3569324094727	25.25153115549311	25.226639359510173	25.164897075524014
37	25.506915667303875	24.98507378070062	24.94228823813919	24.565722313856313
38	24.522139525160203	24.866992769863387	25.474405741589496	25.136461963386914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	46.0
1	58.0
2	70.0
3	70.0
4	335.5
5	601.0
6	601.0
7	884.0
8	1167.0
9	1308.0
10	1449.0
11	1449.0
12	1753.0
13	2057.0
14	2598.0
15	3139.0
16	3139.0
17	4863.0
18	6587.0
19	6587.0
20	7422.0
21	8257.0
22	8509.5
23	8762.0
24	8762.0
25	10143.5
26	11525.0
27	11525.0
28	14924.0
29	18323.0
30	22602.5
31	26882.0
32	26882.0
33	33468.5
34	40055.0
35	40055.0
36	43814.0
37	47573.0
38	54528.0
39	61483.0
40	61483.0
41	64424.0
42	67365.0
43	75925.0
44	84485.0
45	84485.0
46	88108.5
47	91732.0
48	91732.0
49	96954.5
50	102177.0
51	102335.0
52	102493.0
53	102493.0
54	98174.0
55	93855.0
56	93855.0
57	92403.0
58	90951.0
59	81739.5
60	72528.0
61	72528.0
62	69038.5
63	65549.0
64	54502.0
65	43455.0
66	43455.0
67	36564.5
68	29674.0
69	29674.0
70	24136.0
71	18598.0
72	14688.0
73	10778.0
74	10778.0
75	8134.5
76	5491.0
77	5491.0
78	5374.0
79	5257.0
80	4040.5
81	2824.0
82	2824.0
83	2611.5
84	2399.0
85	2399.0
86	1570.0
87	741.0
88	580.0
89	419.0
90	419.0
91	249.5
92	80.0
93	55.5
94	31.0
95	31.0
96	21.5
97	12.0
98	12.0
99	14.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007175215212164913
2	0.0
3	0.0
4	1.7716580770777562E-4
5	0.0
6	1.7716580770777562E-4
7	7.086632308311025E-4
8	0.002303155500201083
9	0.002391738404054971
10	0.0010629948462466537
11	0.004429145192694391
12	3.5433161541555125E-4
13	8.858290385388781E-5
14	0.0
15	0.0
16	8.858290385388781E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	3.5433161541555125E-4
22	0.0
23	2.6574871156166344E-4
24	0.0
25	8.858290385388781E-5
26	8.858290385388781E-5
27	1.7716580770777562E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1128886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.680598757622185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.99057293959598	36.75895966058646
2	15.401942504253146	15.303554512835346
3	5.082694618068359	7.575339357833396
4	2.1292100443693123	4.231217195400429
5	1.0672295088928574	2.651030050680011
6	0.5904410870652488	1.7600080041901736
7	0.3673309249611344	1.277445420598224
8	0.24351980650200813	0.9678567837088049
9	0.16535675839493263	0.739352048911175
>10	0.7491461400955719	6.8047746665918245
>50	0.09141345028447735	3.2381117596560705
>100	0.10358018118123721	10.835021572393616
>500	0.01272351612091736	4.187607787362558
>1k	0.004838520214996742	3.6697211792520044
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4681	0.4146565729400488	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3466	0.3070283447575752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2037	0.18044337515036948	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1728	0.15307125785951814	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1641	0.14536454522422992	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1555	0.13774641549279557	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1534	0.1358861745118639	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1461	0.1294196225305301	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1440	0.12755938154959845	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1406	0.12454756281856628	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1397	0.12375031668388128	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1393	0.12339598506846572	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1384	0.12259873893378075	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1329	0.1177266792218169	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1262	0.11179162466360643	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1257	0.11134871014433698	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1239	0.109754217874967	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1171	0.10373058041290263	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1169	0.10355341460519486	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1159	0.10266758556665599	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1139	0.10089592748957822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.858290385388781E-5
2	0.0	0.0	0.0	0.0	8.858290385388781E-5
3	0.0	0.0	0.0	0.0	8.858290385388781E-5
4	0.0	0.0	0.0	0.0	8.858290385388781E-5
5	0.0	0.0	0.0	0.0	8.858290385388781E-5
6	0.0	0.0	0.0	0.0	8.858290385388781E-5
7	0.0	0.0	0.0	0.0	8.858290385388781E-5
8	0.0	0.0	0.0	0.0	8.858290385388781E-5
9	0.0	0.0	0.0	0.0	8.858290385388781E-5
10	0.0	0.0	0.0	0.0	8.858290385388781E-5
11	0.0	0.0	0.0	0.0	8.858290385388781E-5
12	0.0	0.0	0.0	0.0	8.858290385388781E-5
13	0.0	0.0	0.0	0.0	8.858290385388781E-5
14	0.0	0.0	0.0	0.0	8.858290385388781E-5
15	0.0	0.0	0.0	0.0	2.6574871156166344E-4
16	0.0	0.0	0.0	1.7716580770777562E-4	2.6574871156166344E-4
17	0.0	0.0	0.0	1.7716580770777562E-4	2.6574871156166344E-4
18	0.0	0.0	0.0	2.6574871156166344E-4	3.5433161541555125E-4
19	0.0	0.0	0.0	2.6574871156166344E-4	3.5433161541555125E-4
20	0.0	0.0	0.0	5.314974231233269E-4	4.4291451926943906E-4
21	0.0	0.0	0.0	7.086632308311025E-4	5.314974231233269E-4
22	0.0	0.0	0.0	9.744119423927659E-4	5.314974231233269E-4
23	0.0	0.0	0.0	0.0016830751732238684	5.314974231233269E-4
24	0.0	0.0	0.0	0.0025689042117627466	5.314974231233269E-4
25	0.0	0.0	0.0	0.002923235827178298	5.314974231233269E-4
26	0.0	0.0	0.0	0.0031889845387399612	6.200803269772147E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGT	40	0.004482139	20.000998	4
CGAATGT	75	1.5090773E-6	19.199255	23
TCTAGCG	340	0.0	18.822798	28
ATAGACG	45	0.0088498965	17.777876	3
TTCGATA	55	0.0013649794	17.453869	12
TAGCGGC	360	0.0	17.33266	30
ACCGAAC	65	2.0988505E-4	17.232393	8
CTAGCGG	365	0.0	17.095228	29
GTAGGAC	415	0.0	16.96395	3
GATATAC	255	0.0	16.94202	1
TCGCGTA	190	0.0	16.84369	9
CATCGTT	315	0.0	16.761255	28
ACGAACG	185	0.0	16.431795	15
CAAGACG	410	0.0	16.39106	4
ATCGTTT	325	0.0	16.245523	29
AAGACGG	435	0.0	16.186148	5
CCGTCCA	60	0.0024403352	16.001507	9
GCGTAAC	190	0.0	16.000797	11
CTAAACG	60	0.0024425008	15.999381	12
CGAACGA	190	0.0	15.99938	16
>>END_MODULE