FastQCFastQC Report
Thu 2 Feb 2017
SRR4062812_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062812_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664113
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34270.5160266400446912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23090.34768179511619257No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14900.22435940871508311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13680.20598904102163335No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11170.16819426814412608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.15117909151002917No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8490.12783968993228562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGGT200.003729220232.040491
TCGACGC450.00884728717.77748514
CGCAAGA1650.017.4568862
CTATTCC2050.017.1678644
GTATTAG1404.0017767E-1117.1645471
TATGACA854.985466E-616.9383474
GTCCTAT2150.016.3928071
CCTATTC2050.016.388743
GTTCTAG600.002419935816.0202451
ATATTAC600.002419935816.0202451
CGCGTAA908.5712145E-615.99973710
TCGCGTA908.5712145E-615.9997379
GCGTAAC1001.3125518E-615.99973611
AGACGGA1800.015.9973296
CAAGACG1800.015.9973294
ACGACGG1102.019242E-715.9973286
CAGAGCG1850.015.5673114
ACGGACC1753.6379788E-1215.541438
TCCAATA1551.8735591E-1015.4859492
TACGACG1255.1355528E-815.3574345
Produced by FastQC (version 0.11.5)